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Bee venom ameliorates lipopolysaccharide-induced memory loss by preventing NF-kappaB pathway.
J Neuroinflammation. 2015 Jun 26; 12:124.JN

Abstract

BACKGROUND

Accumulation of beta-amyloid and neuroinflammation trigger Alzheimer's disease. We previously found that lipopolysaccharide (LPS) caused neuroinflammation with concomitant accumulation of beta-amyloid peptides leading to memory loss. A variety of anti-inflammatory compounds inhibiting nuclear factor kappaB (NF-κB) activation have showed efficacy to hinder neuroinflammation and amyloidogenesis. We also found that bee venom (BV) inhibits NF-κB.

METHODS

A mouse model of LPS-induced memory loss used administration of BV (0.8 and 1.6 μg/kg/day, i.p.) to ICR mice for 7 days before injection of LPS (2.5 mg/kg/day, i.p.). Memory loss was assessed using a Morris water maze test and passive avoidance test. For in vitro study, we treated BV (0.5, 1, and 2 μg/mL) to astrocytes and microglial BV-2 cells with LPS (1 μg/mL).

RESULTS

We found that BV inhibited LPS-induced memory loss determined by behavioral tests as well as cell death. BV also inhibited LPS-induced increases in the level of beta-amyloid (Aβ), β-and γ-secretases activities, NF-κB and its DNA-binding activity and expression of APP, and BACE1 and neuroinflammation proteins (COX-2, iNOS, GFAP and IBA-1) in the brain and cultured cells. In addition, pull-down assay and molecular modeling showed that BV binds to NF-κB.

CONCLUSIONS

BV attenuates LPS-induced amyloidogenesis, neuroinflammation, and therefore memory loss via inhibiting NF-κB signaling pathway. Thus, BV could be useful for treatment of Alzheimer's disease.

Authors+Show Affiliations

College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaemgmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju, Chungbuk, 361-951, Republic of Korea.College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaemgmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju, Chungbuk, 361-951, Republic of Korea.College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaemgmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju, Chungbuk, 361-951, Republic of Korea.College of Oriental Medicine, Gachon University, San 65, Bokjeong-dong, Sujeong-gu, Seongnam, Gyeonggii-do, 461-701, Republic of Korea.Department of Food Science & Technology, Korea National University of Transportation, Jeungpyeong, 368-701, Republic of Korea.College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaemgmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju, Chungbuk, 361-951, Republic of Korea.College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaemgmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju, Chungbuk, 361-951, Republic of Korea.Department of Chemistry, Utah Valley University, 800 W University Pkwy, Orem, UT, 84058, USA.Department of Obstetrics and Gynecology, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 64 Daeheung-ro, Jung gu, Daejeon, 301-723, Republic of Korea.College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaemgmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju, Chungbuk, 361-951, Republic of Korea. sondj1@chungbuk.ac.kr.College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaemgmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju, Chungbuk, 361-951, Republic of Korea. jinthong@chungbuk.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26112466

Citation

Gu, Sun Mi, et al. "Bee Venom Ameliorates Lipopolysaccharide-induced Memory Loss By Preventing NF-kappaB Pathway." Journal of Neuroinflammation, vol. 12, 2015, p. 124.
Gu SM, Park MH, Hwang CJ, et al. Bee venom ameliorates lipopolysaccharide-induced memory loss by preventing NF-kappaB pathway. J Neuroinflammation. 2015;12:124.
Gu, S. M., Park, M. H., Hwang, C. J., Song, H. S., Lee, U. S., Han, S. B., Oh, K. W., Ham, Y. W., Song, M. J., Son, D. J., & Hong, J. T. (2015). Bee venom ameliorates lipopolysaccharide-induced memory loss by preventing NF-kappaB pathway. Journal of Neuroinflammation, 12, 124. https://doi.org/10.1186/s12974-015-0344-2
Gu SM, et al. Bee Venom Ameliorates Lipopolysaccharide-induced Memory Loss By Preventing NF-kappaB Pathway. J Neuroinflammation. 2015 Jun 26;12:124. PubMed PMID: 26112466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bee venom ameliorates lipopolysaccharide-induced memory loss by preventing NF-kappaB pathway. AU - Gu,Sun Mi, AU - Park,Mi Hee, AU - Hwang,Chul Ju, AU - Song,Ho Sueb, AU - Lee,Ung Soo, AU - Han,Sang Bae, AU - Oh,Ki Wan, AU - Ham,Young Wan, AU - Song,Min Jong, AU - Son,Dong Ju, AU - Hong,Jin Tae, Y1 - 2015/06/26/ PY - 2015/04/09/received PY - 2015/06/15/accepted PY - 2015/6/27/entrez PY - 2015/6/27/pubmed PY - 2016/5/4/medline SP - 124 EP - 124 JF - Journal of neuroinflammation JO - J Neuroinflammation VL - 12 N2 - BACKGROUND: Accumulation of beta-amyloid and neuroinflammation trigger Alzheimer's disease. We previously found that lipopolysaccharide (LPS) caused neuroinflammation with concomitant accumulation of beta-amyloid peptides leading to memory loss. A variety of anti-inflammatory compounds inhibiting nuclear factor kappaB (NF-κB) activation have showed efficacy to hinder neuroinflammation and amyloidogenesis. We also found that bee venom (BV) inhibits NF-κB. METHODS: A mouse model of LPS-induced memory loss used administration of BV (0.8 and 1.6 μg/kg/day, i.p.) to ICR mice for 7 days before injection of LPS (2.5 mg/kg/day, i.p.). Memory loss was assessed using a Morris water maze test and passive avoidance test. For in vitro study, we treated BV (0.5, 1, and 2 μg/mL) to astrocytes and microglial BV-2 cells with LPS (1 μg/mL). RESULTS: We found that BV inhibited LPS-induced memory loss determined by behavioral tests as well as cell death. BV also inhibited LPS-induced increases in the level of beta-amyloid (Aβ), β-and γ-secretases activities, NF-κB and its DNA-binding activity and expression of APP, and BACE1 and neuroinflammation proteins (COX-2, iNOS, GFAP and IBA-1) in the brain and cultured cells. In addition, pull-down assay and molecular modeling showed that BV binds to NF-κB. CONCLUSIONS: BV attenuates LPS-induced amyloidogenesis, neuroinflammation, and therefore memory loss via inhibiting NF-κB signaling pathway. Thus, BV could be useful for treatment of Alzheimer's disease. SN - 1742-2094 UR - https://www.unboundmedicine.com/medline/citation/26112466/Bee_venom_ameliorates_lipopolysaccharide_induced_memory_loss_by_preventing_NF_kappaB_pathway_ L2 - https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-015-0344-2 DB - PRIME DP - Unbound Medicine ER -