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Polyunsaturated fatty acid metabolism in a marine teleost, Nibe croaker Nibea mitsukurii: Functional characterization of Fads2 desaturase and Elovl5 and Elovl4 elongases.

Abstract

To reduce the requirement for fish oil in marine aquaculture, it would be advantageous to endow marine fish species with the capability for the endogenous biosynthesis of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). For this purpose, we have previously produced transgenic Nibe croaker (Nibea mitsukurii) carrying an elongase of very-long-chain fatty acids 2 (elovl2) gene isolated from Masu salmon (Oncorhynchus masou). However, fatty acid analysis revealed that 24:5n-3 accumulated in the liver of the transgenic fish, whereas the DHA level did not differ between non-transgenic and transgenic fish. Therefore, to select more effective enzymes for successful transgenic synthesis of DHA, understanding the endogenous DHA biosynthetic pathway in the Nibe croaker is considered to be important. The present study aimed to investigate the biochemical functions of the Elovl5, Elovl4 and Fads2 enzymes involved in the DHA biosynthetic pathway in the Nibe croaker. The results showed that both Elovl5 and Elovl4 were able to elongate C18 fatty acids to C22 fatty acids and that Fads2 had Δ6 desaturase activity toward C18 fatty acids and weak Δ8 desaturase activity toward C20 fatty acids. On the other hand, Fads2 was found to lack the ability to convert 24:5n-3 to 24:6n-3, a fatty acid that can directly be converted to DHA via β-oxidation.

Authors+Show Affiliations

Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, 90 Sippy Downs Dr, Sippy Downs, QLD 4556, Australia.Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, 90 Sippy Downs Dr, Sippy Downs, QLD 4556, Australia.Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.Research Center for Advanced Science and Technology, Tokyo University of Marine Science and Technology, Banda 670, Tateyama-shi, Chiba 294-0308, Japan.Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan. Electronic address: goro@kaiyodai.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26112824

Citation

Kabeya, Naoki, et al. "Polyunsaturated Fatty Acid Metabolism in a Marine Teleost, Nibe Croaker Nibea Mitsukurii: Functional Characterization of Fads2 Desaturase and Elovl5 and Elovl4 Elongases." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 188, 2015, pp. 37-45.
Kabeya N, Yamamoto Y, Cummins SF, et al. Polyunsaturated fatty acid metabolism in a marine teleost, Nibe croaker Nibea mitsukurii: Functional characterization of Fads2 desaturase and Elovl5 and Elovl4 elongases. Comp Biochem Physiol B, Biochem Mol Biol. 2015;188:37-45.
Kabeya, N., Yamamoto, Y., Cummins, S. F., Elizur, A., Yazawa, R., Takeuchi, Y., ... Yoshizaki, G. (2015). Polyunsaturated fatty acid metabolism in a marine teleost, Nibe croaker Nibea mitsukurii: Functional characterization of Fads2 desaturase and Elovl5 and Elovl4 elongases. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 188, pp. 37-45. doi:10.1016/j.cbpb.2015.06.005.
Kabeya N, et al. Polyunsaturated Fatty Acid Metabolism in a Marine Teleost, Nibe Croaker Nibea Mitsukurii: Functional Characterization of Fads2 Desaturase and Elovl5 and Elovl4 Elongases. Comp Biochem Physiol B, Biochem Mol Biol. 2015;188:37-45. PubMed PMID: 26112824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyunsaturated fatty acid metabolism in a marine teleost, Nibe croaker Nibea mitsukurii: Functional characterization of Fads2 desaturase and Elovl5 and Elovl4 elongases. AU - Kabeya,Naoki, AU - Yamamoto,Yoji, AU - Cummins,Scott F, AU - Elizur,Abigail, AU - Yazawa,Ryosuke, AU - Takeuchi,Yutaka, AU - Haga,Yutaka, AU - Satoh,Shuichi, AU - Yoshizaki,Goro, Y1 - 2015/06/23/ PY - 2015/02/02/received PY - 2015/06/17/revised PY - 2015/06/17/accepted PY - 2015/6/27/entrez PY - 2015/6/27/pubmed PY - 2015/12/31/medline KW - Desaturase KW - Docosahexaenoic acid KW - Eicosapentaenoic acid KW - Elongase KW - Marine fish SP - 37 EP - 45 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp. Biochem. Physiol. B, Biochem. Mol. Biol. VL - 188 N2 - To reduce the requirement for fish oil in marine aquaculture, it would be advantageous to endow marine fish species with the capability for the endogenous biosynthesis of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). For this purpose, we have previously produced transgenic Nibe croaker (Nibea mitsukurii) carrying an elongase of very-long-chain fatty acids 2 (elovl2) gene isolated from Masu salmon (Oncorhynchus masou). However, fatty acid analysis revealed that 24:5n-3 accumulated in the liver of the transgenic fish, whereas the DHA level did not differ between non-transgenic and transgenic fish. Therefore, to select more effective enzymes for successful transgenic synthesis of DHA, understanding the endogenous DHA biosynthetic pathway in the Nibe croaker is considered to be important. The present study aimed to investigate the biochemical functions of the Elovl5, Elovl4 and Fads2 enzymes involved in the DHA biosynthetic pathway in the Nibe croaker. The results showed that both Elovl5 and Elovl4 were able to elongate C18 fatty acids to C22 fatty acids and that Fads2 had Δ6 desaturase activity toward C18 fatty acids and weak Δ8 desaturase activity toward C20 fatty acids. On the other hand, Fads2 was found to lack the ability to convert 24:5n-3 to 24:6n-3, a fatty acid that can directly be converted to DHA via β-oxidation. SN - 1879-1107 UR - https://www.unboundmedicine.com/medline/citation/26112824/Polyunsaturated_fatty_acid_metabolism_in_a_marine_teleost_Nibe_croaker_Nibea_mitsukurii:_Functional_characterization_of_Fads2_desaturase_and_Elovl5_and_Elovl4_elongases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-4959(15)00118-9 DB - PRIME DP - Unbound Medicine ER -