TY - JOUR T1 - Online polar two phase countercurrent chromatography×high performance liquid chromatography for preparative isolation of polar polyphenols from tea extract in a single step. AU - Chen,Wei-Bin, AU - Li,Shu-Qi, AU - Chen,Long-Jiang, AU - Fang,Mei-Juan, AU - Chen,Quan-Cheng, AU - Wu,Zhen, AU - Wu,Yun-Long, AU - Qiu,Ying-Kun, Y1 - 2015/06/15/ PY - 2014/12/16/received PY - 2015/04/03/revised PY - 2015/06/12/accepted PY - 2015/6/27/entrez PY - 2015/6/27/pubmed PY - 2016/1/14/medline KW - Counter-current chromatography KW - Polar two-phase solvent system KW - Tea polyphenols KW - Tieguanyin tea KW - Two-dimensional liquid chromatography SP - 179 EP - 86 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 997 N2 - Herein, we report an on-line two-dimensional system constructed by counter-current chromatography (CCC) coupling with preparative high-performance liquid chromatography (prep-HPLC) for the separation and purification of polar natural products. The CCC was used as the first dimensional isolation column, where an environmental friendly polar two-phase solvent system of isopropanol and 16% sodium chloride aqueous solution (1:1.2, v/v) was introduced for low toxicity and favorable resolution. In addition, by applying the stop-and-go flow technique, effluents pre-fractionated by CCC was further purified by a preparative column packed with octadecyl silane (ODS) as the second dimension. The interface between the two dimensions was comprised of a 6-port switching valve and an electronically controlled 2-position 10-port switching valve connected with two equivalent holding columns. To be highlighted here, this rationally designed interface for the purpose of smooth desalination, absorption and desorption, successfully solved the solvent compatibility problem between the two dimensional separation systems. The present integrated system was successfully applied in a one-step preparative separation and identification of 10 pure compounds from the water extracts of Tieguanyin tea (Chinese oolong tea). In short, all the results demonstrated that the on-line 2D CCC×LC method is an efficient and green approach for harvesting polar targets in a single step, which showed great promise in drug discovery. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/26114653/Online_polar_two_phase_countercurrent_chromatography×high_performance_liquid_chromatography_for_preparative_isolation_of_polar_polyphenols_from_tea_extract_in_a_single_step_ DB - PRIME DP - Unbound Medicine ER -