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Purification of an antitumor-active, branched (1----3)-beta-D-glucan from Volvariella volvacea, and elucidation of its fine structure.
Carbohydr Res. 1989 Oct 31; 193:227-39.CR

Abstract

A (1--3)-beta-D-glucan branched by O-6 substitution (FCAP), obtained from the cold-alkali extract of the fruiting body of V. volvacea, exhibited potent growth-inhibitory activity against implanted tumors in mice. It contained protein and appeared to be heterogeneous. Fractionation by DEAE-Toyopearl column chromatography yielded an unbound, protein-free glucan fraction ([alpha]D - 30 degrees in M NaOH, mol. wt. 1.5-2 x 10(6)), which showed the highest antitumor activity. The polysaccharide had a moderately branched structure, consisting of a backbone chain of beta-(1--3)-linked-D-glucose residues, one out of five or six being substituted at O-6 with single glucosyl of beta-(1--6)-linked diglucosyl groups. Digestion of the glucan with exo-(1--3)-beta-D-glucanase yielded glucose and ++gentiobiose (molar ratio, 8:2:1.0), and a highly branched (d.b. 1/3), degraded glucan. Digestion with endo-(1--3)-beta-D-glucanase gave D-glucose, laminarabiose, a trisaccharide beta-D-Glcp-(1--6)-beta-D-Glcp-(1--3)-D-Glc, a tetrasaccharide beta-D-Glcp-(1--3)-beta-D-Glcp-(1--3)-[beta-D-Glcp-(1--6)]-D-Glc, and a highly branched (d.b. 1/2), enzyme-resistant glucan. The results suggest that the Volvariella glucan is structurally heterogeneous with regard to the distribution of branches, having less branched, moderately branched, and highly branched segments.

Authors+Show Affiliations

Department of Food and Nutrition, Faculty of Science of Living, Osaka City University, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2611783

Citation

Kishida, E, et al. "Purification of an Antitumor-active, Branched (1----3)-beta-D-glucan From Volvariella Volvacea, and Elucidation of Its Fine Structure." Carbohydrate Research, vol. 193, 1989, pp. 227-39.
Kishida E, Sone Y, Misaki A. Purification of an antitumor-active, branched (1----3)-beta-D-glucan from Volvariella volvacea, and elucidation of its fine structure. Carbohydr Res. 1989;193:227-39.
Kishida, E., Sone, Y., & Misaki, A. (1989). Purification of an antitumor-active, branched (1----3)-beta-D-glucan from Volvariella volvacea, and elucidation of its fine structure. Carbohydrate Research, 193, 227-39.
Kishida E, Sone Y, Misaki A. Purification of an Antitumor-active, Branched (1----3)-beta-D-glucan From Volvariella Volvacea, and Elucidation of Its Fine Structure. Carbohydr Res. 1989 Oct 31;193:227-39. PubMed PMID: 2611783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification of an antitumor-active, branched (1----3)-beta-D-glucan from Volvariella volvacea, and elucidation of its fine structure. AU - Kishida,E, AU - Sone,Y, AU - Misaki,A, PY - 1989/10/31/pubmed PY - 1989/10/31/medline PY - 1989/10/31/entrez SP - 227 EP - 39 JF - Carbohydrate research JO - Carbohydr Res VL - 193 N2 - A (1--3)-beta-D-glucan branched by O-6 substitution (FCAP), obtained from the cold-alkali extract of the fruiting body of V. volvacea, exhibited potent growth-inhibitory activity against implanted tumors in mice. It contained protein and appeared to be heterogeneous. Fractionation by DEAE-Toyopearl column chromatography yielded an unbound, protein-free glucan fraction ([alpha]D - 30 degrees in M NaOH, mol. wt. 1.5-2 x 10(6)), which showed the highest antitumor activity. The polysaccharide had a moderately branched structure, consisting of a backbone chain of beta-(1--3)-linked-D-glucose residues, one out of five or six being substituted at O-6 with single glucosyl of beta-(1--6)-linked diglucosyl groups. Digestion of the glucan with exo-(1--3)-beta-D-glucanase yielded glucose and ++gentiobiose (molar ratio, 8:2:1.0), and a highly branched (d.b. 1/3), degraded glucan. Digestion with endo-(1--3)-beta-D-glucanase gave D-glucose, laminarabiose, a trisaccharide beta-D-Glcp-(1--6)-beta-D-Glcp-(1--3)-D-Glc, a tetrasaccharide beta-D-Glcp-(1--3)-beta-D-Glcp-(1--3)-[beta-D-Glcp-(1--6)]-D-Glc, and a highly branched (d.b. 1/2), enzyme-resistant glucan. The results suggest that the Volvariella glucan is structurally heterogeneous with regard to the distribution of branches, having less branched, moderately branched, and highly branched segments. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/2611783/Purification_of_an_antitumor_active_branched__1____3__beta_D_glucan_from_Volvariella_volvacea_and_elucidation_of_its_fine_structure_ DB - PRIME DP - Unbound Medicine ER -