Tags

Type your tag names separated by a space and hit enter

Purification of an antitumor-active, branched (1----3)-beta-D-glucan from Volvariella volvacea, and elucidation of its fine structure.
Carbohydr Res. 1989 Oct 31; 193:227-39.CR

Abstract

A (1--3)-beta-D-glucan branched by O-6 substitution (FCAP), obtained from the cold-alkali extract of the fruiting body of V. volvacea, exhibited potent growth-inhibitory activity against implanted tumors in mice. It contained protein and appeared to be heterogeneous. Fractionation by DEAE-Toyopearl column chromatography yielded an unbound, protein-free glucan fraction ([alpha]D - 30 degrees in M NaOH, mol. wt. 1.5-2 x 10(6)), which showed the highest antitumor activity. The polysaccharide had a moderately branched structure, consisting of a backbone chain of beta-(1--3)-linked-D-glucose residues, one out of five or six being substituted at O-6 with single glucosyl of beta-(1--6)-linked diglucosyl groups. Digestion of the glucan with exo-(1--3)-beta-D-glucanase yielded glucose and ++gentiobiose (molar ratio, 8:2:1.0), and a highly branched (d.b. 1/3), degraded glucan. Digestion with endo-(1--3)-beta-D-glucanase gave D-glucose, laminarabiose, a trisaccharide beta-D-Glcp-(1--6)-beta-D-Glcp-(1--3)-D-Glc, a tetrasaccharide beta-D-Glcp-(1--3)-beta-D-Glcp-(1--3)-[beta-D-Glcp-(1--6)]-D-Glc, and a highly branched (d.b. 1/2), enzyme-resistant glucan. The results suggest that the Volvariella glucan is structurally heterogeneous with regard to the distribution of branches, having less branched, moderately branched, and highly branched segments.

Authors+Show Affiliations

Kishida E
Department of Food and Nutrition, Faculty of Science of Living, Osaka City University, Japan.
Sone Y
No affiliation info available
Misaki A
No affiliation info available

MeSH

AgaricalesAnimalsAntineoplastic AgentsCarbohydrate SequenceChemical FractionationChromatography, Ion ExchangeFemaleGlucansHydrolysisMethylationMiceMice, Inbred ICRMolecular Sequence DataMolecular WeightSarcoma 180

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2611783

Citation

Kishida, E, et al. "Purification of an Antitumor-active, Branched (1----3)-beta-D-glucan From Volvariella Volvacea, and Elucidation of Its Fine Structure." Carbohydrate Research, vol. 193, 1989, pp. 227-39.
Kishida E, Sone Y, Misaki A. Purification of an antitumor-active, branched (1----3)-beta-D-glucan from Volvariella volvacea, and elucidation of its fine structure. Carbohydr Res. 1989;193:227-39.
Kishida, E., Sone, Y., & Misaki, A. (1989). Purification of an antitumor-active, branched (1----3)-beta-D-glucan from Volvariella volvacea, and elucidation of its fine structure. Carbohydrate Research, 193, 227-39.
Kishida E, Sone Y, Misaki A. Purification of an Antitumor-active, Branched (1----3)-beta-D-glucan From Volvariella Volvacea, and Elucidation of Its Fine Structure. Carbohydr Res. 1989 Oct 31;193:227-39. PubMed PMID: 2611783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification of an antitumor-active, branched (1----3)-beta-D-glucan from Volvariella volvacea, and elucidation of its fine structure. AU - Kishida,E, AU - Sone,Y, AU - Misaki,A, PY - 1989/10/31/pubmed PY - 1989/10/31/medline PY - 1989/10/31/entrez SP - 227 EP - 39 JF - Carbohydrate research JO - Carbohydr Res VL - 193 N2 - A (1--3)-beta-D-glucan branched by O-6 substitution (FCAP), obtained from the cold-alkali extract of the fruiting body of V. volvacea, exhibited potent growth-inhibitory activity against implanted tumors in mice. It contained protein and appeared to be heterogeneous. Fractionation by DEAE-Toyopearl column chromatography yielded an unbound, protein-free glucan fraction ([alpha]D - 30 degrees in M NaOH, mol. wt. 1.5-2 x 10(6)), which showed the highest antitumor activity. The polysaccharide had a moderately branched structure, consisting of a backbone chain of beta-(1--3)-linked-D-glucose residues, one out of five or six being substituted at O-6 with single glucosyl of beta-(1--6)-linked diglucosyl groups. Digestion of the glucan with exo-(1--3)-beta-D-glucanase yielded glucose and ++gentiobiose (molar ratio, 8:2:1.0), and a highly branched (d.b. 1/3), degraded glucan. Digestion with endo-(1--3)-beta-D-glucanase gave D-glucose, laminarabiose, a trisaccharide beta-D-Glcp-(1--6)-beta-D-Glcp-(1--3)-D-Glc, a tetrasaccharide beta-D-Glcp-(1--3)-beta-D-Glcp-(1--3)-[beta-D-Glcp-(1--6)]-D-Glc, and a highly branched (d.b. 1/2), enzyme-resistant glucan. The results suggest that the Volvariella glucan is structurally heterogeneous with regard to the distribution of branches, having less branched, moderately branched, and highly branched segments. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/2611783/Purification_of_an_antitumor_active_branched__1____3__beta_D_glucan_from_Volvariella_volvacea_and_elucidation_of_its_fine_structure_ DB - PRIME DP - Unbound Medicine ER -