TY - JOUR T1 - Nicotine and metabolites determination in human plasma by ultra performance liquid chromatography-tandem mass spectrometry: a simple approach for solving contamination problem and clinical application. AU - Liachenko,Natalia, AU - Boulamery,Audrey, AU - Simon,Nicolas, Y1 - 2015/08/11/ PY - 2015/01/23/received PY - 2015/04/26/revised PY - 2015/05/18/accepted PY - 2015/6/30/entrez PY - 2015/6/30/pubmed PY - 2016/7/2/medline KW - UPLC/MS-MS KW - activated charcoal KW - cotinine KW - nicotine KW - trans-3′-hydroxycotinine SP - 499 EP - 509 JF - Fundamental & clinical pharmacology JO - Fundam Clin Pharmacol VL - 29 IS - 5 N2 - A quantitative method using ultra performance liquid chromatography-tandem mass spectrometry is described for simultaneous determination of nicotine and its metabolites (cotinine and trans-3'- hydroxycotinine) in human plasma. Aliquots of 0.25 mL of plasma specimens were used for analysis, and 3 analytes were extracted by liquid-liquid extraction. The main problem was blank plasma contamination with environmental nicotine. Activated charcoal was used to avoid this analytical interference. For optimized chromatographic performance, a basic mobile phase consisting of 0.2% ammonia in water (mobile phase A, pH10.6) and acetonitrile (mobile phase B) was selected. The analytes were separated on a 50 mm × 2.1 mm BEH C18 column, 1.7 μm particle size, and quantified by MS/MS using multiple-reaction monitoring (MRM) in positive mode. The chromatographic separation was achieved in 3 min followed by 1.2 min of column equilibration. The calibration curves were linear in the concentration range of 10-1000 ng/mL with correlation coefficients exceeding 0.99. Within-day precisions and between-day precisions (CV, %) were <15 %. The accuracy expressed as bias was within ±15% for all analytes. The recovery values ranged from 50% to 97%. The ions used for quantification of nicotine, cotinine and 3-OH-cotinine were 166.9 > 129.7; 176.9 > 79.7; 192.9 > 79.7 m/z, respectively. The original blank sample preparation solved the problem of contamination in a cost-effective and efficient way. The validated method has been routinely used for analysis of nicotine and metabolites and determination of hydroxycotinine/cotinine metabolic ratio. This biomarker seems to be interesting at predicting response of nicotine patch replacement therapies. SN - 1472-8206 UR - https://www.unboundmedicine.com/medline/citation/26118829/Nicotine_and_metabolites_determination_in_human_plasma_by_ultra_performance_liquid_chromatography_tandem_mass_spectrometry:_a_simple_approach_for_solving_contamination_problem_and_clinical_application_ DB - PRIME DP - Unbound Medicine ER -