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Highly exposed Pt nanoparticles supported on porous graphene for electrochemical detection of hydrogen peroxide in living cells.
Biosens Bioelectron. 2015 Dec 15; 74:71-7.BB

Abstract

In this study, we developed a novel biosensor based on highly exposed Pt nanoparticles (Pt NPs) decorated porous graphene (PG) for the reliable detection of extracellular hydrogen peroxide (H2O2) released from living cells. The commercially available low-cost hydrophilic CaCO3 spheres were used as template for preparing PG. The porous structure provided larger surface area and more active sites. Due to the porous structure of PG, the Pt NPs supported on PG were not secluded by aggregated graphene layers and were highly exposed to target molecules. Ultrafine Pt NPs were well dispersed and loaded on PG by a method of microwave assistance. Electrochemical performances of the Pt/PG nanocomposites modified glassy carbon electrode (GCE) were investigated. The electrocatalytic reduction of H2O2 showed a wide linear range from 1 to 1477 μM, with a high sensitivity of 341.14 μA mM(-1) cm(-2) and a limit of detection (LOD) as low as 0.50 μM. Moreover, the Pt/PG/GCE exhibited excellent anti-interference property, reproducibility and long-term storage stability. Because of these remarkable analytical advantages, the constructed sensor was used to determine H2O2 released from living cells with satisfactory results. The superior catalytic activity makes Pt/PG nanocomposites a promising candidate for electrochemical sensors and biosensors design.

Authors+Show Affiliations

Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China.Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China. Electronic address: baoxj133@nenu.edu.cn.School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, PR China.Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China. Electronic address: guolp078@nenu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26120812

Citation

Liu, Jian, et al. "Highly Exposed Pt Nanoparticles Supported On Porous Graphene for Electrochemical Detection of Hydrogen Peroxide in Living Cells." Biosensors & Bioelectronics, vol. 74, 2015, pp. 71-7.
Liu J, Bo X, Zhao Z, et al. Highly exposed Pt nanoparticles supported on porous graphene for electrochemical detection of hydrogen peroxide in living cells. Biosens Bioelectron. 2015;74:71-7.
Liu, J., Bo, X., Zhao, Z., & Guo, L. (2015). Highly exposed Pt nanoparticles supported on porous graphene for electrochemical detection of hydrogen peroxide in living cells. Biosensors & Bioelectronics, 74, 71-7. https://doi.org/10.1016/j.bios.2015.06.042
Liu J, et al. Highly Exposed Pt Nanoparticles Supported On Porous Graphene for Electrochemical Detection of Hydrogen Peroxide in Living Cells. Biosens Bioelectron. 2015 Dec 15;74:71-7. PubMed PMID: 26120812.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly exposed Pt nanoparticles supported on porous graphene for electrochemical detection of hydrogen peroxide in living cells. AU - Liu,Jian, AU - Bo,Xiangjie, AU - Zhao,Zheng, AU - Guo,Liping, Y1 - 2015/06/20/ PY - 2015/04/14/received PY - 2015/06/16/revised PY - 2015/06/18/accepted PY - 2015/6/30/entrez PY - 2015/6/30/pubmed PY - 2016/6/10/medline KW - Cell tracking KW - Electrochemical biosensor KW - Nonenzymatic hydrogen peroxide detection KW - Porous graphene KW - Pt nanoparticles SP - 71 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 74 N2 - In this study, we developed a novel biosensor based on highly exposed Pt nanoparticles (Pt NPs) decorated porous graphene (PG) for the reliable detection of extracellular hydrogen peroxide (H2O2) released from living cells. The commercially available low-cost hydrophilic CaCO3 spheres were used as template for preparing PG. The porous structure provided larger surface area and more active sites. Due to the porous structure of PG, the Pt NPs supported on PG were not secluded by aggregated graphene layers and were highly exposed to target molecules. Ultrafine Pt NPs were well dispersed and loaded on PG by a method of microwave assistance. Electrochemical performances of the Pt/PG nanocomposites modified glassy carbon electrode (GCE) were investigated. The electrocatalytic reduction of H2O2 showed a wide linear range from 1 to 1477 μM, with a high sensitivity of 341.14 μA mM(-1) cm(-2) and a limit of detection (LOD) as low as 0.50 μM. Moreover, the Pt/PG/GCE exhibited excellent anti-interference property, reproducibility and long-term storage stability. Because of these remarkable analytical advantages, the constructed sensor was used to determine H2O2 released from living cells with satisfactory results. The superior catalytic activity makes Pt/PG nanocomposites a promising candidate for electrochemical sensors and biosensors design. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/26120812/Highly_exposed_Pt_nanoparticles_supported_on_porous_graphene_for_electrochemical_detection_of_hydrogen_peroxide_in_living_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(15)30210-4 DB - PRIME DP - Unbound Medicine ER -