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Protective effect of epigallocatechin gallate, a major constituent of green tea, against renal ischemia-reperfusion injury in rats.
Int Urol Nephrol. 2015 Aug; 47(8):1429-35.IU

Abstract

BACKGROUND

Renal ischemia-reperfusion (I/R) injury plays an important role in the acute kidney injury. The pathogenetic mechanisms potential I/R injury is involved in apoptosis and inflammation. Epigallocatechin gallate (EGCG), a major constituent of green tea, has been shown to have anti-inflammatory and anti-apoptotic activities. This study aimed to explore the underlying effects and mechanisms of EGCG on renal I/R injury in a rat model.

MATERIALS AND METHODS

We induced renal I/R injury in SD rats by clamping the left renal artery for 45 min followed by 24-h reperfusion, along with a contralateral nephrectomy. We randomly allocated 30 rats to three groups (n = 10): sham group, IRI group, and EGCG group. We preconditioned rats intraperitoneally with EGCG (50 mg/kg) or vehicle (50 mg/kg) 45 min before inducing renal ischemia. We collected serum and kidneys at 24 h after reperfusion. Renal function and histologic damage were assessed. We also determined markers of inflammation and apoptosis in kidneys or serum.

RESULTS

EGCG pretreatment can significantly reduce renal dysfunction, histologic change and the expression of tumor necrosis factor-α, IL-1β, IL-6, Bax and cleavage caspase 3 induced by I/R injury and increase the expression of Bax and caspase 3. Moreover, EGCG pretreatment can further induce the activation of p38 mitogen-activated protein kinase in kidney, with no influence on the expression of p38.

CONCLUSIONS

EGCG treatment can decrease renal ischemia-reperfusion injury by suppressing inflammation and cell apoptosis. Thus, EGCG may represent a potential strategy to reduce renal I/R injury.

Authors+Show Affiliations

Department of Nephrology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China, 18280141016@163.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26122117

Citation

Lv, Jun, et al. "Protective Effect of Epigallocatechin Gallate, a Major Constituent of Green Tea, Against Renal Ischemia-reperfusion Injury in Rats." International Urology and Nephrology, vol. 47, no. 8, 2015, pp. 1429-35.
Lv J, Feng M, Zhang L, et al. Protective effect of epigallocatechin gallate, a major constituent of green tea, against renal ischemia-reperfusion injury in rats. Int Urol Nephrol. 2015;47(8):1429-35.
Lv, J., Feng, M., Zhang, L., Wan, X., Zeng, Y. C., Liang, P. F., & Xu, A. P. (2015). Protective effect of epigallocatechin gallate, a major constituent of green tea, against renal ischemia-reperfusion injury in rats. International Urology and Nephrology, 47(8), 1429-35. https://doi.org/10.1007/s11255-015-1030-0
Lv J, et al. Protective Effect of Epigallocatechin Gallate, a Major Constituent of Green Tea, Against Renal Ischemia-reperfusion Injury in Rats. Int Urol Nephrol. 2015;47(8):1429-35. PubMed PMID: 26122117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of epigallocatechin gallate, a major constituent of green tea, against renal ischemia-reperfusion injury in rats. AU - Lv,Jun, AU - Feng,Min, AU - Zhang,LiLi, AU - Wan,Xia, AU - Zeng,Yu Chun, AU - Liang,Pei Fen, AU - Xu,An Ping, Y1 - 2015/06/28/ PY - 2015/03/02/received PY - 2015/06/07/accepted PY - 2015/7/1/entrez PY - 2015/7/1/pubmed PY - 2016/5/18/medline SP - 1429 EP - 35 JF - International urology and nephrology JO - Int Urol Nephrol VL - 47 IS - 8 N2 - BACKGROUND: Renal ischemia-reperfusion (I/R) injury plays an important role in the acute kidney injury. The pathogenetic mechanisms potential I/R injury is involved in apoptosis and inflammation. Epigallocatechin gallate (EGCG), a major constituent of green tea, has been shown to have anti-inflammatory and anti-apoptotic activities. This study aimed to explore the underlying effects and mechanisms of EGCG on renal I/R injury in a rat model. MATERIALS AND METHODS: We induced renal I/R injury in SD rats by clamping the left renal artery for 45 min followed by 24-h reperfusion, along with a contralateral nephrectomy. We randomly allocated 30 rats to three groups (n = 10): sham group, IRI group, and EGCG group. We preconditioned rats intraperitoneally with EGCG (50 mg/kg) or vehicle (50 mg/kg) 45 min before inducing renal ischemia. We collected serum and kidneys at 24 h after reperfusion. Renal function and histologic damage were assessed. We also determined markers of inflammation and apoptosis in kidneys or serum. RESULTS: EGCG pretreatment can significantly reduce renal dysfunction, histologic change and the expression of tumor necrosis factor-α, IL-1β, IL-6, Bax and cleavage caspase 3 induced by I/R injury and increase the expression of Bax and caspase 3. Moreover, EGCG pretreatment can further induce the activation of p38 mitogen-activated protein kinase in kidney, with no influence on the expression of p38. CONCLUSIONS: EGCG treatment can decrease renal ischemia-reperfusion injury by suppressing inflammation and cell apoptosis. Thus, EGCG may represent a potential strategy to reduce renal I/R injury. SN - 1573-2584 UR - https://www.unboundmedicine.com/medline/citation/26122117/Protective_effect_of_epigallocatechin_gallate_a_major_constituent_of_green_tea_against_renal_ischemia_reperfusion_injury_in_rats_ L2 - https://doi.org/10.1007/s11255-015-1030-0 DB - PRIME DP - Unbound Medicine ER -