Tags

Type your tag names separated by a space and hit enter

Natural phenolics greatly increase flax (Linum usitatissimum) oil stability.
BMC Biotechnol. 2015 Jun 30; 15:62.BB

Abstract

BACKGROUND

Flaxseed oil is characterized by high content of essential polyunsaturated fatty acids (PUFA) promoted as a human dietary supplement protecting against atherosclerosis. The disadvantage of the high PUFA content in flax oil is high susceptibility to oxidation, which can result in carcinogenic compound formation. Linola flax cultivar is characterized by high linoleic acid content in comparison to traditional flax cultivars rich in linolenic acid. The changes in fatty acid proportions increase oxidative stability of Linola oil and broaden its use as an edible oil for cooking. However one of investigated transgenic lines has high ALA content making it suitable as omega-3 source. Protection of PUFA oxidation is a critical factor in oil quality. The aim of this study was to investigate the impact of phenylpropanoid contents on the oil properties important during the whole technological process from seed storage to grinding and oil pressing, which may influence health benefits as well as shelf-life, and to establish guidelines for the selection of new cultivars.

METHODS

The composition of oils was determined by chromatographic (GS-FID and LC-PDA-MS) methods. Antioxidant properties of secondary metabolites were analyzed by DPPH method. The stability of oils was investigated: a) during regular storage by measuring acid value peroxide value p-anisidine value malondialdehyde, conjugated dienes and trienes; b) by using accelerated rancidity tests by TBARS reaction; c) by thermoanalytical - differential scanning calorimetry (DSC).

RESULTS

In one approach, in order to increase oil stability, exogenous substances added are mainly lipid soluble antioxidants from the isoprenoid pathway, such as tocopherol and carotene. The other approach is based on transgenic plant generation that accumulates water soluble compounds. Increased accumulation of phenolic compounds in flax seeds was achieved by three different strategies that modify genes coding for enzymes from the phenylpropanoid pathway. The three types of transgenic flax had different phenylpropanoid profiles detected in oil, highly increasing its stability.

CONCLUSIONS

We found that hydrophilic phenylpropanoids more than lipophilic isoprenoid compounds determine oil stability however they can work synergistically. Among phenolics the caffeic acid was most effective in increasing oil stability.

Authors+Show Affiliations

Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland. haska3@vp.pl.Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland. kulma@ibmb.uni.wroc.pl. Linum Foundation, Plac Grunwaldzki 24a, 50-363, Wroclaw, Poland. kulma@ibmb.uni.wroc.pl.Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland. thadeus@ibmb.uni.wroc.pl. Department of Genetics, Plant Breeding and Seed Production, The Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Plant Sciences, Pl. Grunwaldzki 24A, 53-363, Wrocław, Poland. thadeus@ibmb.uni.wroc.pl.Department of Food Science and Dietetics, Wroclaw Medical University, Borowska 211, 50-556, Wroclaw, Poland. aprescha@gmail.com.Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland. mzuk@ibmb.uni.wroc.pl. Linum Foundation, Plac Grunwaldzki 24a, 50-363, Wroclaw, Poland. mzuk@ibmb.uni.wroc.pl.Department of Food Science and Dietetics, Wroclaw Medical University, Borowska 211, 50-556, Wroclaw, Poland. magdalena.grajzer@gmail.com.Faculty of Biotechnology, University of Wroclaw, Fryderyka Joliot-Curie 14a, 50-383, Wrocław, Poland. marcin.lukaszewicz@uni.wroc.pl.Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland. szopa@ibmb.uni.wroc.pl. Linum Foundation, Plac Grunwaldzki 24a, 50-363, Wroclaw, Poland. szopa@ibmb.uni.wroc.pl. Department of Genetics, Plant Breeding and Seed Production, The Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Plant Sciences, Pl. Grunwaldzki 24A, 53-363, Wrocław, Poland. szopa@ibmb.uni.wroc.pl.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26123633

Citation

Hasiewicz-Derkacz, Karolina, et al. "Natural Phenolics Greatly Increase Flax (Linum Usitatissimum) Oil Stability." BMC Biotechnology, vol. 15, 2015, p. 62.
Hasiewicz-Derkacz K, Kulma A, Czuj T, et al. Natural phenolics greatly increase flax (Linum usitatissimum) oil stability. BMC Biotechnol. 2015;15:62.
Hasiewicz-Derkacz, K., Kulma, A., Czuj, T., Prescha, A., Żuk, M., Grajzer, M., Łukaszewicz, M., & Szopa, J. (2015). Natural phenolics greatly increase flax (Linum usitatissimum) oil stability. BMC Biotechnology, 15, 62. https://doi.org/10.1186/s12896-015-0178-0
Hasiewicz-Derkacz K, et al. Natural Phenolics Greatly Increase Flax (Linum Usitatissimum) Oil Stability. BMC Biotechnol. 2015 Jun 30;15:62. PubMed PMID: 26123633.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Natural phenolics greatly increase flax (Linum usitatissimum) oil stability. AU - Hasiewicz-Derkacz,Karolina, AU - Kulma,Anna, AU - Czuj,Tadeusz, AU - Prescha,Anna, AU - Żuk,Magdalena, AU - Grajzer,Magdalena, AU - Łukaszewicz,Marcin, AU - Szopa,Jan, Y1 - 2015/06/30/ PY - 2014/09/25/received PY - 2015/06/01/accepted PY - 2015/7/1/entrez PY - 2015/7/1/pubmed PY - 2016/3/22/medline SP - 62 EP - 62 JF - BMC biotechnology JO - BMC Biotechnol. VL - 15 N2 - BACKGROUND: Flaxseed oil is characterized by high content of essential polyunsaturated fatty acids (PUFA) promoted as a human dietary supplement protecting against atherosclerosis. The disadvantage of the high PUFA content in flax oil is high susceptibility to oxidation, which can result in carcinogenic compound formation. Linola flax cultivar is characterized by high linoleic acid content in comparison to traditional flax cultivars rich in linolenic acid. The changes in fatty acid proportions increase oxidative stability of Linola oil and broaden its use as an edible oil for cooking. However one of investigated transgenic lines has high ALA content making it suitable as omega-3 source. Protection of PUFA oxidation is a critical factor in oil quality. The aim of this study was to investigate the impact of phenylpropanoid contents on the oil properties important during the whole technological process from seed storage to grinding and oil pressing, which may influence health benefits as well as shelf-life, and to establish guidelines for the selection of new cultivars. METHODS: The composition of oils was determined by chromatographic (GS-FID and LC-PDA-MS) methods. Antioxidant properties of secondary metabolites were analyzed by DPPH method. The stability of oils was investigated: a) during regular storage by measuring acid value peroxide value p-anisidine value malondialdehyde, conjugated dienes and trienes; b) by using accelerated rancidity tests by TBARS reaction; c) by thermoanalytical - differential scanning calorimetry (DSC). RESULTS: In one approach, in order to increase oil stability, exogenous substances added are mainly lipid soluble antioxidants from the isoprenoid pathway, such as tocopherol and carotene. The other approach is based on transgenic plant generation that accumulates water soluble compounds. Increased accumulation of phenolic compounds in flax seeds was achieved by three different strategies that modify genes coding for enzymes from the phenylpropanoid pathway. The three types of transgenic flax had different phenylpropanoid profiles detected in oil, highly increasing its stability. CONCLUSIONS: We found that hydrophilic phenylpropanoids more than lipophilic isoprenoid compounds determine oil stability however they can work synergistically. Among phenolics the caffeic acid was most effective in increasing oil stability. SN - 1472-6750 UR - https://www.unboundmedicine.com/medline/citation/26123633/Natural_phenolics_greatly_increase_flax__Linum_usitatissimum__oil_stability_ L2 - https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-015-0178-0 DB - PRIME DP - Unbound Medicine ER -