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Genetic relationships analysis of olive cultivars grown in China.
Genet Mol Res. 2015 Jun 01; 14(2):5958-69.GM

Abstract

The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISSR techniques. Thirty-two samples were collected from Xichang. Five of these cultivars were issued from a Chinese breeding program. Genomic DNA samples were extracted from young leaves and PCR was used to generate SSR and ISSR markers. A total of 107 polymorphic bands were detected on thirteen SSR loci, with an average of eight alleles per locus. The observed heterozygosity ranged from 0.785 (DCA03) to 0.990 (GAPU47), and the expected heterozygosity varied between 0.782 (DCA03) and 0.940 (GAPU103A). The discrimination power ranged from 0.57 to 0.83, while the polymorphism information content values ranged from 0.768 (DCA03) to 0.934 (GAPU103A). Nine ISSR primers generated 85 reproducible bands of which 78 (91.8%) were polymorphic. Based on our data, genetic similarity between cultivars ranged from 0.57 to 0.83. Cluster analysis revealed that 32 cultivars were clustered into six groups, which supports similar morphology such as use, oil content and fruit weight but not similar geographical origins. Our data also allow the identification of unknown cultivars and cases of synonyms.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhan MM
No affiliation info available
Cheng ZZ
No affiliation info available
Su GC
Liang Shan Zhong Ze New Technology Development Co., Ltd., Xichang, China.
Wang AY
Liang Shan Zhong Ze New Technology Development Co., Ltd., Xichang, China.
Chen HP
Liang Shan Zhong Ze New Technology Development Co., Ltd., Xichang, China.
Shan Z
No affiliation info available
Yang ZS
No affiliation info available
Huang QM
No affiliation info available

MeSH

ChinaDNA FingerprintingGenotypeMicrosatellite RepeatsOleaPhylogenyPolymorphism, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26125795

Citation

Zhan, M M., et al. "Genetic Relationships Analysis of Olive Cultivars Grown in China." Genetics and Molecular Research : GMR, vol. 14, no. 2, 2015, pp. 5958-69.
Zhan MM, Cheng ZZ, Su GC, et al. Genetic relationships analysis of olive cultivars grown in China. Genet Mol Res. 2015;14(2):5958-69.
Zhan, M. M., Cheng, Z. Z., Su, G. C., Wang, A. Y., Chen, H. P., Shan, Z., Yang, Z. S., & Huang, Q. M. (2015). Genetic relationships analysis of olive cultivars grown in China. Genetics and Molecular Research : GMR, 14(2), 5958-69. https://doi.org/10.4238/2015.June.1.13
Zhan MM, et al. Genetic Relationships Analysis of Olive Cultivars Grown in China. Genet Mol Res. 2015 Jun 1;14(2):5958-69. PubMed PMID: 26125795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic relationships analysis of olive cultivars grown in China. AU - Zhan,M M, AU - Cheng,Z Z, AU - Su,G C, AU - Wang,A Y, AU - Chen,H P, AU - Shan,Z, AU - Yang,Z S, AU - Huang,Q M, Y1 - 2015/06/01/ PY - 2015/7/1/entrez PY - 2015/7/1/pubmed PY - 2016/4/14/medline SP - 5958 EP - 69 JF - Genetics and molecular research : GMR JO - Genet Mol Res VL - 14 IS - 2 N2 - The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISSR techniques. Thirty-two samples were collected from Xichang. Five of these cultivars were issued from a Chinese breeding program. Genomic DNA samples were extracted from young leaves and PCR was used to generate SSR and ISSR markers. A total of 107 polymorphic bands were detected on thirteen SSR loci, with an average of eight alleles per locus. The observed heterozygosity ranged from 0.785 (DCA03) to 0.990 (GAPU47), and the expected heterozygosity varied between 0.782 (DCA03) and 0.940 (GAPU103A). The discrimination power ranged from 0.57 to 0.83, while the polymorphism information content values ranged from 0.768 (DCA03) to 0.934 (GAPU103A). Nine ISSR primers generated 85 reproducible bands of which 78 (91.8%) were polymorphic. Based on our data, genetic similarity between cultivars ranged from 0.57 to 0.83. Cluster analysis revealed that 32 cultivars were clustered into six groups, which supports similar morphology such as use, oil content and fruit weight but not similar geographical origins. Our data also allow the identification of unknown cultivars and cases of synonyms. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/26125795/Genetic_relationships_analysis_of_olive_cultivars_grown_in_China_ DB - PRIME DP - Unbound Medicine ER -