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The sesquiterpene lactone polymatin B from Smallanthus sonchifolius induces different cell death mechanisms in three cancer cell lines.
Phytochemistry. 2015 Sep; 117:332-339.P

Abstract

A 8β-angeloyloxy-9α-hydroxy-14-oxo-acanthospermolide and five known melampolide sesquiterpene lactones (uvedalin, enhydrin, polymatin B, sonchifolin, and fluctuanin) were isolated from the leaves of Smallanthus sonchifolius. The compounds were identified by 1D-, 2D-NMR, HRMS, IR and UV analyses. In vitro cytotoxicity assays (MTT) showed that these sesquiterpene lactones display poor cytotoxic effects on peripheral blood mononuclear cells (PBMC) of healthy human subjects, whereas a strong cytotoxicity was observed in leukemia and pancreas cancer cells. For the mechanism of action of polymatin B, oxidative stress seems to be involved. Interestingly, reactive oxygen species (ROS) formation mainly induced different effects: apoptosis in CCRF-CEM cells, necroptosis in CEM-ADR5000 cells through induction of RIP1K, neither apoptosis nor necroptosis in MIA-PaCa-2 cells. Additionally, cells also died partly by necrosis.

Authors+Show Affiliations

Department of Pharmaceutical Biology and Biotechnology, Faculty of Chemistry and Pharmacy, University of Freiburg, Stefan-Meier-Str. 19 (VF), D-79104 Freiburg, Germany; Spemann Graduate School of Biology and Medicine (SGBM), Albert Ludwigs University Freiburg, Albertstrasse 19a, D-79104 Freiburg, Germany.Cátedra de Farmacognosia, IQUIMEFA (UBA-CONICET), Facultad de Farmacia y Bioquímica, UBA, Junín 956, Buenos Aires 1113, Argentina.INQUINOA (CONICET), Facultad de Bioquímica, Química y Farmacia, UNT, Ayacucho 971 (T4000INI), San Miguel de Tucumán, Argentina.Facultad de Ciencias Naturales, Instituto de Ecología Regional (IER), Universidad Nacional de Tucumán, C. C. 34, 4107 Yerba Buena, Tucumán, Argentina.Cátedra de Farmacognosia, IQUIMEFA (UBA-CONICET), Facultad de Farmacia y Bioquímica, UBA, Junín 956, Buenos Aires 1113, Argentina.Cátedra de Farmacognosia, IQUIMEFA (UBA-CONICET), Facultad de Farmacia y Bioquímica, UBA, Junín 956, Buenos Aires 1113, Argentina. Electronic address: lmusch@ffyb.uba.ar.Department of Pharmaceutical Biology and Biotechnology, Faculty of Chemistry and Pharmacy, University of Freiburg, Stefan-Meier-Str. 19 (VF), D-79104 Freiburg, Germany; Spemann Graduate School of Biology and Medicine (SGBM), Albert Ludwigs University Freiburg, Albertstrasse 19a, D-79104 Freiburg, Germany.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26125943

Citation

De Ford, Christian, et al. "The Sesquiterpene Lactone Polymatin B From Smallanthus Sonchifolius Induces Different Cell Death Mechanisms in Three Cancer Cell Lines." Phytochemistry, vol. 117, 2015, pp. 332-339.
De Ford C, Ulloa JL, Catalán CAN, et al. The sesquiterpene lactone polymatin B from Smallanthus sonchifolius induces different cell death mechanisms in three cancer cell lines. Phytochemistry. 2015;117:332-339.
De Ford, C., Ulloa, J. L., Catalán, C. A. N., Grau, A., Martino, V. S., Muschietti, L. V., & Merfort, I. (2015). The sesquiterpene lactone polymatin B from Smallanthus sonchifolius induces different cell death mechanisms in three cancer cell lines. Phytochemistry, 117, 332-339. https://doi.org/10.1016/j.phytochem.2015.06.020
De Ford C, et al. The Sesquiterpene Lactone Polymatin B From Smallanthus Sonchifolius Induces Different Cell Death Mechanisms in Three Cancer Cell Lines. Phytochemistry. 2015;117:332-339. PubMed PMID: 26125943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The sesquiterpene lactone polymatin B from Smallanthus sonchifolius induces different cell death mechanisms in three cancer cell lines. AU - De Ford,Christian, AU - Ulloa,Jerónimo L, AU - Catalán,César A N, AU - Grau,Alfredo, AU - Martino,Virginia S, AU - Muschietti,Liliana V, AU - Merfort,Irmgard, Y1 - 2015/06/27/ PY - 2014/08/23/received PY - 2015/06/05/revised PY - 2015/06/19/accepted PY - 2015/7/1/entrez PY - 2015/7/1/pubmed PY - 2016/6/21/medline KW - Asteraceae KW - Cytotoxicity KW - Sesquiterpene lactones KW - Smallanthus sonchifolius SP - 332 EP - 339 JF - Phytochemistry JO - Phytochemistry VL - 117 N2 - A 8β-angeloyloxy-9α-hydroxy-14-oxo-acanthospermolide and five known melampolide sesquiterpene lactones (uvedalin, enhydrin, polymatin B, sonchifolin, and fluctuanin) were isolated from the leaves of Smallanthus sonchifolius. The compounds were identified by 1D-, 2D-NMR, HRMS, IR and UV analyses. In vitro cytotoxicity assays (MTT) showed that these sesquiterpene lactones display poor cytotoxic effects on peripheral blood mononuclear cells (PBMC) of healthy human subjects, whereas a strong cytotoxicity was observed in leukemia and pancreas cancer cells. For the mechanism of action of polymatin B, oxidative stress seems to be involved. Interestingly, reactive oxygen species (ROS) formation mainly induced different effects: apoptosis in CCRF-CEM cells, necroptosis in CEM-ADR5000 cells through induction of RIP1K, neither apoptosis nor necroptosis in MIA-PaCa-2 cells. Additionally, cells also died partly by necrosis. SN - 1873-3700 UR - https://www.unboundmedicine.com/medline/citation/26125943/The_sesquiterpene_lactone_polymatin_B_from_Smallanthus_sonchifolius_induces_different_cell_death_mechanisms_in_three_cancer_cell_lines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(15)30031-5 DB - PRIME DP - Unbound Medicine ER -