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Pan-Genome Analysis of Brazilian Lineage A Amoebal Mimiviruses.
Viruses. 2015 Jun 26; 7(7):3483-99.V

Abstract

Since the recent discovery of Samba virus, the first representative of the family Mimiviridae from Brazil, prospecting for mimiviruses has been conducted in different environmental conditions in Brazil. Recently, we isolated using Acanthamoeba sp. three new mimiviruses, all of lineage A of amoebal mimiviruses: Kroon virus from urban lake water; Amazonia virus from the Brazilian Amazon river; and Oyster virus from farmed oysters. The aims of this work were to sequence and analyze the genome of these new Brazilian mimiviruses (mimi-BR) and update the analysis of the Samba virus genome. The genomes of Samba virus, Amazonia virus and Oyster virus were 97%-99% similar, whereas Kroon virus had a low similarity (90%-91%) with other mimi-BR. A total of 3877 proteins encoded by mimi-BR were grouped into 974 orthologous clusters. In addition, we identified three new ORFans in the Kroon virus genome. Additional work is needed to expand our knowledge of the diversity of mimiviruses from Brazil, including if and why among amoebal mimiviruses those of lineage A predominate in the Brazilian environment.

Authors+Show Affiliations

Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. felipelopesassis@gmail.com.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. leenaasd@gmail.com. Department of Biochemistry, Faculty of Science, King Abdulaziz University, 21589 Jeddah, Saudi Arabia. leenaasd@gmail.com.Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. jonatas.abrahao@gmail.com.Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. ernagkroon@gmail.com.Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. fabiopiod154@gmail.com.Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. ketyllen@yahoo.com.br.Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. pvboratto@gmail.com.Centro de Ciências Biológicas, Departamento de Microbiologia e Parasitologia, Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Florianópolis, 88040-900 Santa Catarina, Brazil. maryrp@gmail.com.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. catherine.robert@univ-amu.fr.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. benamarsamia@yahoo.fr.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. bernard.la-scola@univ-amu.fr. Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Assistance Publique-Hôpitaux de Marseille, Centre Hospitalo-Universitaire Timone, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, 13385 Marseille, France. bernard.la-scola@univ-amu.fr.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. philippe.colson@univ-amu.fr. Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Assistance Publique-Hôpitaux de Marseille, Centre Hospitalo-Universitaire Timone, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, 13385 Marseille, France. philippe.colson@univ-amu.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26131958

Citation

Assis, Felipe L., et al. "Pan-Genome Analysis of Brazilian Lineage a Amoebal Mimiviruses." Viruses, vol. 7, no. 7, 2015, pp. 3483-99.
Assis FL, Bajrai L, Abrahao JS, et al. Pan-Genome Analysis of Brazilian Lineage A Amoebal Mimiviruses. Viruses. 2015;7(7):3483-99.
Assis, F. L., Bajrai, L., Abrahao, J. S., Kroon, E. G., Dornas, F. P., Andrade, K. R., Boratto, P. V., Pilotto, M. R., Robert, C., Benamar, S., Scola, B. L., & Colson, P. (2015). Pan-Genome Analysis of Brazilian Lineage A Amoebal Mimiviruses. Viruses, 7(7), 3483-99. https://doi.org/10.3390/v7072782
Assis FL, et al. Pan-Genome Analysis of Brazilian Lineage a Amoebal Mimiviruses. Viruses. 2015 Jun 26;7(7):3483-99. PubMed PMID: 26131958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pan-Genome Analysis of Brazilian Lineage A Amoebal Mimiviruses. AU - Assis,Felipe L, AU - Bajrai,Leena, AU - Abrahao,Jonatas S, AU - Kroon,Erna G, AU - Dornas,Fabio P, AU - Andrade,Kétyllen R, AU - Boratto,Paulo V M, AU - Pilotto,Mariana R, AU - Robert,Catherine, AU - Benamar,Samia, AU - Scola,Bernard La, AU - Colson,Philippe, Y1 - 2015/06/26/ PY - 2015/04/03/received PY - 2015/06/15/revised PY - 2015/06/18/accepted PY - 2015/7/2/entrez PY - 2015/7/2/pubmed PY - 2016/4/20/medline KW - Amazonia virus KW - Kroon virus KW - Megavirales KW - Mimiviridae KW - Samba virus KW - genomics KW - mimivirus KW - oyster virus KW - pan-genome SP - 3483 EP - 99 JF - Viruses JO - Viruses VL - 7 IS - 7 N2 - Since the recent discovery of Samba virus, the first representative of the family Mimiviridae from Brazil, prospecting for mimiviruses has been conducted in different environmental conditions in Brazil. Recently, we isolated using Acanthamoeba sp. three new mimiviruses, all of lineage A of amoebal mimiviruses: Kroon virus from urban lake water; Amazonia virus from the Brazilian Amazon river; and Oyster virus from farmed oysters. The aims of this work were to sequence and analyze the genome of these new Brazilian mimiviruses (mimi-BR) and update the analysis of the Samba virus genome. The genomes of Samba virus, Amazonia virus and Oyster virus were 97%-99% similar, whereas Kroon virus had a low similarity (90%-91%) with other mimi-BR. A total of 3877 proteins encoded by mimi-BR were grouped into 974 orthologous clusters. In addition, we identified three new ORFans in the Kroon virus genome. Additional work is needed to expand our knowledge of the diversity of mimiviruses from Brazil, including if and why among amoebal mimiviruses those of lineage A predominate in the Brazilian environment. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/26131958/Pan_Genome_Analysis_of_Brazilian_Lineage_A_Amoebal_Mimiviruses_ L2 - https://www.mdpi.com/resolver?pii=v7072782 DB - PRIME DP - Unbound Medicine ER -