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A multiparental cross population for mapping QTL for agronomic traits in durum wheat (Triticum turgidum ssp. durum).
Plant Biotechnol J. 2016 Feb; 14(2):735-48.PB

Abstract

Multiparental cross designs for mapping quantitative trait loci (QTL) provide an efficient alternative to biparental populations because of their broader genetic basis and potentially higher mapping resolution. We describe the development and deployment of a recombinant inbred line (RIL) population in durum wheat (Triticum turgidum ssp. durum) obtained by crossing four elite cultivars. A linkage map spanning 2664 cM and including 7594 single nucleotide polymorphisms (SNPs) was produced by genotyping 338 RILs. QTL analysis was carried out by both interval mapping on founder haplotype probabilities and SNP bi-allelic tests for heading date and maturity date, plant height and grain yield from four field experiments. Sixteen QTL were identified across environments and detection methods, including two yield QTL on chromosomes 2BL and 7AS, with the former mapped independently from the photoperiod response gene Ppd-B1, while the latter overlapped with the vernalization locus VRN-A3. Additionally, 21 QTL with environment-specific effects were found. Our results indicated a prevalence of environment-specific QTL with relatively small effect on the control of grain yield. For all traits, functionally different QTL alleles in terms of direction and size of genetic effect were distributed among parents. We showed that QTL results based on founder haplotypes closely matched functional alleles at known heading date loci. Despite the four founders, only 2.1 different functional haplotypes were estimated per QTL, on average. This durum wheat population provides a mapping resource for detailed genetic dissection of agronomic traits in an elite background typical of breeding programmes.

Authors+Show Affiliations

Department of Agricultural Sciences, University of Bologna, Bologna, Italy.Department of Agricultural Sciences, University of Bologna, Bologna, Italy.Digital Productivity Flagship and Agriculture Flagship, CSIRO, St Lucia, Qld, Australia.Società Produttori Sementi Bologna, Argelato, Bologna, Italy.Società Produttori Sementi Bologna, Argelato, Bologna, Italy.Department of Agricultural Sciences, University of Bologna, Bologna, Italy.Department of Agricultural Sciences, University of Bologna, Bologna, Italy.Department of Agricultural Sciences, University of Bologna, Bologna, Italy.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26132599

Citation

Milner, Sara Giulia, et al. "A Multiparental Cross Population for Mapping QTL for Agronomic Traits in Durum Wheat (Triticum Turgidum Ssp. Durum)." Plant Biotechnology Journal, vol. 14, no. 2, 2016, pp. 735-48.
Milner SG, Maccaferri M, Huang BE, et al. A multiparental cross population for mapping QTL for agronomic traits in durum wheat (Triticum turgidum ssp. durum). Plant Biotechnol J. 2016;14(2):735-48.
Milner, S. G., Maccaferri, M., Huang, B. E., Mantovani, P., Massi, A., Frascaroli, E., Tuberosa, R., & Salvi, S. (2016). A multiparental cross population for mapping QTL for agronomic traits in durum wheat (Triticum turgidum ssp. durum). Plant Biotechnology Journal, 14(2), 735-48. https://doi.org/10.1111/pbi.12424
Milner SG, et al. A Multiparental Cross Population for Mapping QTL for Agronomic Traits in Durum Wheat (Triticum Turgidum Ssp. Durum). Plant Biotechnol J. 2016;14(2):735-48. PubMed PMID: 26132599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A multiparental cross population for mapping QTL for agronomic traits in durum wheat (Triticum turgidum ssp. durum). AU - Milner,Sara Giulia, AU - Maccaferri,Marco, AU - Huang,Bevan Emma, AU - Mantovani,Paola, AU - Massi,Andrea, AU - Frascaroli,Elisabetta, AU - Tuberosa,Roberto, AU - Salvi,Silvio, Y1 - 2015/07/01/ PY - 2014/11/05/received PY - 2015/04/08/revised PY - 2015/04/29/accepted PY - 2015/7/2/entrez PY - 2015/7/2/pubmed PY - 2016/12/15/medline KW - QTL KW - SNP KW - Triticum turgidum KW - agronomic traits KW - founder haplotypes KW - multiparental cross SP - 735 EP - 48 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 14 IS - 2 N2 - Multiparental cross designs for mapping quantitative trait loci (QTL) provide an efficient alternative to biparental populations because of their broader genetic basis and potentially higher mapping resolution. We describe the development and deployment of a recombinant inbred line (RIL) population in durum wheat (Triticum turgidum ssp. durum) obtained by crossing four elite cultivars. A linkage map spanning 2664 cM and including 7594 single nucleotide polymorphisms (SNPs) was produced by genotyping 338 RILs. QTL analysis was carried out by both interval mapping on founder haplotype probabilities and SNP bi-allelic tests for heading date and maturity date, plant height and grain yield from four field experiments. Sixteen QTL were identified across environments and detection methods, including two yield QTL on chromosomes 2BL and 7AS, with the former mapped independently from the photoperiod response gene Ppd-B1, while the latter overlapped with the vernalization locus VRN-A3. Additionally, 21 QTL with environment-specific effects were found. Our results indicated a prevalence of environment-specific QTL with relatively small effect on the control of grain yield. For all traits, functionally different QTL alleles in terms of direction and size of genetic effect were distributed among parents. We showed that QTL results based on founder haplotypes closely matched functional alleles at known heading date loci. Despite the four founders, only 2.1 different functional haplotypes were estimated per QTL, on average. This durum wheat population provides a mapping resource for detailed genetic dissection of agronomic traits in an elite background typical of breeding programmes. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/26132599/A_multiparental_cross_population_for_mapping_QTL_for_agronomic_traits_in_durum_wheat__Triticum_turgidum_ssp__durum__ L2 - https://doi.org/10.1111/pbi.12424 DB - PRIME DP - Unbound Medicine ER -