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Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL.
Theor Appl Genet. 2015 Oct; 128(10):2067-76.TA

Abstract

KEY MESSAGE

Three novel QTL for peroxidase activity were mapped, and gene-specific markers for TaPod-A1 were developed and validated using RILs derived from the Doumai/Shi 4185 cross and 281 wheat cultivars. TaPod-A1 is within one of the three QTL. Peroxidase (POD) activity in grain is an important factor determining the color of flour and end-use products of wheat, such as noodles and steamed bread. Mapping QTL for POD activity, characterization of POD genes and development of gene-specific markers are important for molecular marker-assisted selection in wheat breeding. Quantitative trait loci (QTL) for POD activity in common wheat were mapped using a recombinant inbred line (RIL) population derived from a Doumai/Shi 4185 cross grown in four environments and genotyped using the wheat 90 K iSelect assay. Three novel QTL for POD activity, QPod.caas-3AL, QPod.caas-4BS and QPod.caas-5AS, were identified on chromosomes 3AL, 4BS and 5AS, explaining 5.3-21.2% of phenotypic variance across environments. The full-length genomic DNA (gDNA) sequence of a POD gene, designated TaPod-A1, on chromosome 3A was characterized by homolog cloning and PCR verification. Two complementary dominant sequence-tagged site (STS) markers, POD-3A1 and POD-3A2, were developed based on single nucleotide polymorphisms (SNPs) between two alleles at the TaPod-A1 locus, amplifying 291- and 766-bp fragments in cultivars with lower and higher POD activities, respectively. The two gene-specific markers were mapped on chromosome 3AL using a set of Chinese Spring (CS) nulli-tetrasomic lines, and ditelosomic lines 3AL and 3AS. QTL analysis indicated that QPod.caas-3AL co-segregated with the gene-specific markers POD-3A1 and POD-3A2. POD-3A1 and POD-3A2 were verified on 281 wheat cultivars and advanced lines, and showed significant (P < 0.05) associations with POD activities. POD-3A1 and POD-3A2 may be useful as markers for improving color attributes in wheat breeding programs.

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Authors+Show Affiliations

Wei J
National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Geng H
College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, Xinjiang, 830052, China.
Zhang Y
National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Liu J
National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Wen W
College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, Xinjiang, 830052, China.
Zhang Y
National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Xia X
National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. xiaxianchun@caas.cn.
Chen X
National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. chenxinmin@caas.cn.
He Z
National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. zhhecaas@163.com. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China. zhhecaas@163.com.

MeSH

AllelesChromosome MappingChromosomes, PlantCrosses, GeneticDNA, PlantGenetic MarkersGenotypePeroxidasesPhenotypePlant BreedingQuantitative Trait LociSequence Tagged SitesTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26133734

Citation

Wei, Jingxin, et al. "Mapping Quantitative Trait Loci for Peroxidase Activity and Developing Gene-specific Markers for TaPod-A1 On Wheat Chromosome 3AL." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 128, no. 10, 2015, pp. 2067-76.
Wei J, Geng H, Zhang Y, et al. Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL. Theor Appl Genet. 2015;128(10):2067-76.
Wei, J., Geng, H., Zhang, Y., Liu, J., Wen, W., Zhang, Y., Xia, X., Chen, X., & He, Z. (2015). Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 128(10), 2067-76. https://doi.org/10.1007/s00122-015-2567-0
Wei J, et al. Mapping Quantitative Trait Loci for Peroxidase Activity and Developing Gene-specific Markers for TaPod-A1 On Wheat Chromosome 3AL. Theor Appl Genet. 2015;128(10):2067-76. PubMed PMID: 26133734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL. AU - Wei,Jingxin, AU - Geng,Hongwei, AU - Zhang,Yan, AU - Liu,Jindong, AU - Wen,Weie, AU - Zhang,Yong, AU - Xia,Xianchun, AU - Chen,Xinmin, AU - He,Zhonghu, Y1 - 2015/07/02/ PY - 2014/12/22/received PY - 2015/06/16/accepted PY - 2015/7/3/entrez PY - 2015/7/3/pubmed PY - 2015/12/24/medline SP - 2067 EP - 76 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 128 IS - 10 N2 - KEY MESSAGE: Three novel QTL for peroxidase activity were mapped, and gene-specific markers for TaPod-A1 were developed and validated using RILs derived from the Doumai/Shi 4185 cross and 281 wheat cultivars. TaPod-A1 is within one of the three QTL. Peroxidase (POD) activity in grain is an important factor determining the color of flour and end-use products of wheat, such as noodles and steamed bread. Mapping QTL for POD activity, characterization of POD genes and development of gene-specific markers are important for molecular marker-assisted selection in wheat breeding. Quantitative trait loci (QTL) for POD activity in common wheat were mapped using a recombinant inbred line (RIL) population derived from a Doumai/Shi 4185 cross grown in four environments and genotyped using the wheat 90 K iSelect assay. Three novel QTL for POD activity, QPod.caas-3AL, QPod.caas-4BS and QPod.caas-5AS, were identified on chromosomes 3AL, 4BS and 5AS, explaining 5.3-21.2% of phenotypic variance across environments. The full-length genomic DNA (gDNA) sequence of a POD gene, designated TaPod-A1, on chromosome 3A was characterized by homolog cloning and PCR verification. Two complementary dominant sequence-tagged site (STS) markers, POD-3A1 and POD-3A2, were developed based on single nucleotide polymorphisms (SNPs) between two alleles at the TaPod-A1 locus, amplifying 291- and 766-bp fragments in cultivars with lower and higher POD activities, respectively. The two gene-specific markers were mapped on chromosome 3AL using a set of Chinese Spring (CS) nulli-tetrasomic lines, and ditelosomic lines 3AL and 3AS. QTL analysis indicated that QPod.caas-3AL co-segregated with the gene-specific markers POD-3A1 and POD-3A2. POD-3A1 and POD-3A2 were verified on 281 wheat cultivars and advanced lines, and showed significant (P < 0.05) associations with POD activities. POD-3A1 and POD-3A2 may be useful as markers for improving color attributes in wheat breeding programs. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/26133734/Mapping_quantitative_trait_loci_for_peroxidase_activity_and_developing_gene_specific_markers_for_TaPod_A1_on_wheat_chromosome_3AL_ L2 - https://dx.doi.org/10.1007/s00122-015-2567-0 DB - PRIME DP - Unbound Medicine ER -