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Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells.

Abstract

An epidemiological study showed that green tea consumption is associated with a reduced risk of hematopoietic malignancy. The major green tea polyphenol epigallocatechin‑3-O-gallate (EGCG) is reported to have anticancer effects. Chronic myeloid leukemia (CML) is a major hematopoietic malignancy characterized by expansion of myeloid cells. In the present study, we showed EGCG-induced acid sphingomyelinase (ASM) activation and lipid raft clustering in CML cells. The ASM inhibitor desipramine significantly reduced EGCG-induced cell death. Protein kinase Cδ is a well‑known kinase that plays an important role in ASM activation. We observed EGCG-induced phosphorylation of protein kinase Cδ at Ser664. Importantly, EGCG-induced ASM activation was significantly reduced by pretreatment of CML cells with the soluble guanylate cyclase inhibitor NS2028, suggesting that EGCG induced ASM activation through the cyclic guanosine monophosphate (cGMP)-dependent pathway. Indeed, pharmacological inhibition of a cGMP-negative regulator enhanced the anti-CML effect of EGCG. These results indicate that EGCG-induced cell death via the cGMP/ASM pathway in CML cells.

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    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

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    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    ,

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.

    Source

    Oncology reports 34:3 2015 Sep pg 1162-8

    MeSH

    Anticarcinogenic Agents
    Blotting, Western
    Catechin
    Cell Line, Tumor
    Enzyme Activation
    Humans
    Leukemia, Myelogenous, Chronic, BCR-ABL Positive
    Membrane Microdomains
    Sphingomyelin Phosphodiesterase
    Tea

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    26135316

    Citation

    Huang, Yuhui, et al. "Green Tea Polyphenol epigallocatechin-O-gallate Induces Cell Death By Acid Sphingomyelinase Activation in Chronic Myeloid Leukemia Cells." Oncology Reports, vol. 34, no. 3, 2015, pp. 1162-8.
    Huang Y, Kumazoe M, Bae J, et al. Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells. Oncol Rep. 2015;34(3):1162-8.
    Huang, Y., Kumazoe, M., Bae, J., Yamada, S., Takai, M., Hidaka, S., ... Tachibana, H. (2015). Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells. Oncology Reports, 34(3), pp. 1162-8. doi:10.3892/or.2015.4086.
    Huang Y, et al. Green Tea Polyphenol epigallocatechin-O-gallate Induces Cell Death By Acid Sphingomyelinase Activation in Chronic Myeloid Leukemia Cells. Oncol Rep. 2015;34(3):1162-8. PubMed PMID: 26135316.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells. AU - Huang,Yuhui, AU - Kumazoe,Motofumi, AU - Bae,Jaehoon, AU - Yamada,Shuhei, AU - Takai,Mika, AU - Hidaka,Shiori, AU - Yamashita,Shuya, AU - Kim,Yoonhee, AU - Won,Yeongseon, AU - Murata,Motoki, AU - Tsukamoto,Shuntaro, AU - Tachibana,Hirofumi, Y1 - 2015/06/26/ PY - 2015/04/25/received PY - 2015/05/29/accepted PY - 2015/7/3/entrez PY - 2015/7/3/pubmed PY - 2016/6/2/medline SP - 1162 EP - 8 JF - Oncology reports JO - Oncol. Rep. VL - 34 IS - 3 N2 - An epidemiological study showed that green tea consumption is associated with a reduced risk of hematopoietic malignancy. The major green tea polyphenol epigallocatechin‑3-O-gallate (EGCG) is reported to have anticancer effects. Chronic myeloid leukemia (CML) is a major hematopoietic malignancy characterized by expansion of myeloid cells. In the present study, we showed EGCG-induced acid sphingomyelinase (ASM) activation and lipid raft clustering in CML cells. The ASM inhibitor desipramine significantly reduced EGCG-induced cell death. Protein kinase Cδ is a well‑known kinase that plays an important role in ASM activation. We observed EGCG-induced phosphorylation of protein kinase Cδ at Ser664. Importantly, EGCG-induced ASM activation was significantly reduced by pretreatment of CML cells with the soluble guanylate cyclase inhibitor NS2028, suggesting that EGCG induced ASM activation through the cyclic guanosine monophosphate (cGMP)-dependent pathway. Indeed, pharmacological inhibition of a cGMP-negative regulator enhanced the anti-CML effect of EGCG. These results indicate that EGCG-induced cell death via the cGMP/ASM pathway in CML cells. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/26135316/full_citation L2 - http://www.spandidos-publications.com/or/34/3/1162 DB - PRIME DP - Unbound Medicine ER -