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Inability of rat DPP4 to allow MERS-CoV infection revealed by using a VSV pseudotype bearing truncated MERS-CoV spike protein.
Arch Virol. 2015 Sep; 160(9):2293-300.AV

Abstract

Middle East respiratory syndrome (MERS) coronavirus (Co-V) contains a single spike (S) protein, which binds to a receptor molecule, dipeptidyl peptidase 4 (DPP4; also known as CD26), and serves as a neutralizing antigen. Pseudotyped viruses are useful for measuring neutralization titers against highly infectious viruses as well as for studying their mechanism of entry. In this study, we constructed a series of cytoplasmic deletion mutants of MERS-CoV S and compared the efficiency with which they formed pseudotypes with vesicular stomatitis virus. A pseudotype bearing an S protein with the C-terminal 16 amino acids deleted (MERSpv-St16) reached a maximum titer that was approximately tenfold higher than that of a pseudotype bearing a non-truncated full-length S protein. Using MERSpv-St16, we demonstrated the inability of rat DPP4 to serve as a functional receptor for MERS-CoV, suggesting that rats are not susceptible to MERS-CoV infection. This study provides novel information that enhances our understanding of the host range of MERS-CoV.

Authors+Show Affiliations

Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26138557

Citation

Fukuma, Aiko, et al. "Inability of Rat DPP4 to Allow MERS-CoV Infection Revealed By Using a VSV Pseudotype Bearing Truncated MERS-CoV Spike Protein." Archives of Virology, vol. 160, no. 9, 2015, pp. 2293-300.
Fukuma A, Tani H, Taniguchi S, et al. Inability of rat DPP4 to allow MERS-CoV infection revealed by using a VSV pseudotype bearing truncated MERS-CoV spike protein. Arch Virol. 2015;160(9):2293-300.
Fukuma, A., Tani, H., Taniguchi, S., Shimojima, M., Saijo, M., & Fukushi, S. (2015). Inability of rat DPP4 to allow MERS-CoV infection revealed by using a VSV pseudotype bearing truncated MERS-CoV spike protein. Archives of Virology, 160(9), 2293-300. https://doi.org/10.1007/s00705-015-2506-z
Fukuma A, et al. Inability of Rat DPP4 to Allow MERS-CoV Infection Revealed By Using a VSV Pseudotype Bearing Truncated MERS-CoV Spike Protein. Arch Virol. 2015;160(9):2293-300. PubMed PMID: 26138557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inability of rat DPP4 to allow MERS-CoV infection revealed by using a VSV pseudotype bearing truncated MERS-CoV spike protein. AU - Fukuma,Aiko, AU - Tani,Hideki, AU - Taniguchi,Satoshi, AU - Shimojima,Masayuki, AU - Saijo,Masayuki, AU - Fukushi,Shuetsu, Y1 - 2015/07/04/ PY - 2015/05/19/received PY - 2015/06/18/accepted PY - 2015/7/4/entrez PY - 2015/7/4/pubmed PY - 2015/11/11/medline SP - 2293 EP - 300 JF - Archives of virology JO - Arch Virol VL - 160 IS - 9 N2 - Middle East respiratory syndrome (MERS) coronavirus (Co-V) contains a single spike (S) protein, which binds to a receptor molecule, dipeptidyl peptidase 4 (DPP4; also known as CD26), and serves as a neutralizing antigen. Pseudotyped viruses are useful for measuring neutralization titers against highly infectious viruses as well as for studying their mechanism of entry. In this study, we constructed a series of cytoplasmic deletion mutants of MERS-CoV S and compared the efficiency with which they formed pseudotypes with vesicular stomatitis virus. A pseudotype bearing an S protein with the C-terminal 16 amino acids deleted (MERSpv-St16) reached a maximum titer that was approximately tenfold higher than that of a pseudotype bearing a non-truncated full-length S protein. Using MERSpv-St16, we demonstrated the inability of rat DPP4 to serve as a functional receptor for MERS-CoV, suggesting that rats are not susceptible to MERS-CoV infection. This study provides novel information that enhances our understanding of the host range of MERS-CoV. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/26138557/Inability_of_rat_DPP4_to_allow_MERS_CoV_infection_revealed_by_using_a_VSV_pseudotype_bearing_truncated_MERS_CoV_spike_protein_ L2 - https://dx.doi.org/10.1007/s00705-015-2506-z DB - PRIME DP - Unbound Medicine ER -