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A ratiometric fluorescent quantum dots based biosensor for organophosphorus pesticides detection by inner-filter effect.
Biosens Bioelectron. 2015 Dec 15; 74:277-83.BB

Abstract

In this work, we develop a novel and sensitive sensor for the detection of organophosphorus pesticides based on the inner-filter effect (IFE) between gold nanoparticles (AuNPs) and ratiometric fluorescent quantum dots (RF-QDs). The RF-QDs has been designed by hybridizing two differently colored CdTe QDs, in which the red emissive QDs entrapped in the silica sphere acting as the reference signal, and the green emissive QDs covalently attached on the silica surface serving as the response signal.The fluorescence of RF-QDs could be quenched by AuNPs based on IFE. Protamine could effectively turn on the fluorescence due to the electrostatic attraction between protamine and AuNPs. Trypsin can easily hydrolyze protamine, leading to the quench of the fluorescence. Then, the fluorescence could be recovered again by the addition of parathion-methyl (PM) which could inhibit the activity of trypsin. By measuring the fluorescence of RF-QDs, the inhibition efficiency of PM to trypsin activity was evaluated. Under the optimized conditions, the inhibition efficiency was proportional to the logarithm of PM concentration in the range of 0.04-400 ng mL(-1), with a detection limit of 0.018 ng mL(-1). Furthermore, the simple and convenient method had been used for PM detection in environmental and agricultural samples with satisfactory results.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yan X
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Li H
School of Pharmacy, Jilin University, Changchun 130021, China.
Han X
College of Computer Science and Technology, Jilin University, Changchun 130012, China; Talent Development Institute, Daqing Oilfield Company, CNPC, 163000, China.
Su X
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.

MeSH

AnimalsBiosensing TechniquesDrinking WaterEnvironmental PollutantsFluorescenceFluorescent DyesGoldLimit of DetectionMetal NanoparticlesMethyl ParathionMilkOryzaPesticidesQuantum DotsSpectrometry, Fluorescence

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26143468

Citation

Yan, Xu, et al. "A Ratiometric Fluorescent Quantum Dots Based Biosensor for Organophosphorus Pesticides Detection By Inner-filter Effect." Biosensors & Bioelectronics, vol. 74, 2015, pp. 277-83.
Yan X, Li H, Han X, et al. A ratiometric fluorescent quantum dots based biosensor for organophosphorus pesticides detection by inner-filter effect. Biosens Bioelectron. 2015;74:277-83.
Yan, X., Li, H., Han, X., & Su, X. (2015). A ratiometric fluorescent quantum dots based biosensor for organophosphorus pesticides detection by inner-filter effect. Biosensors & Bioelectronics, 74, 277-83. https://doi.org/10.1016/j.bios.2015.06.020
Yan X, et al. A Ratiometric Fluorescent Quantum Dots Based Biosensor for Organophosphorus Pesticides Detection By Inner-filter Effect. Biosens Bioelectron. 2015 Dec 15;74:277-83. PubMed PMID: 26143468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A ratiometric fluorescent quantum dots based biosensor for organophosphorus pesticides detection by inner-filter effect. AU - Yan,Xu, AU - Li,Hongxia, AU - Han,Xiaosong, AU - Su,Xingguang, Y1 - 2015/06/20/ PY - 2015/03/24/received PY - 2015/05/26/revised PY - 2015/06/09/accepted PY - 2015/7/6/entrez PY - 2015/7/6/pubmed PY - 2016/6/10/medline KW - Gold nanoparticle KW - Inner-filter effect KW - Organophosphorus pesticides KW - Ratiometric fluorescent quantum dots SP - 277 EP - 83 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 74 N2 - In this work, we develop a novel and sensitive sensor for the detection of organophosphorus pesticides based on the inner-filter effect (IFE) between gold nanoparticles (AuNPs) and ratiometric fluorescent quantum dots (RF-QDs). The RF-QDs has been designed by hybridizing two differently colored CdTe QDs, in which the red emissive QDs entrapped in the silica sphere acting as the reference signal, and the green emissive QDs covalently attached on the silica surface serving as the response signal.The fluorescence of RF-QDs could be quenched by AuNPs based on IFE. Protamine could effectively turn on the fluorescence due to the electrostatic attraction between protamine and AuNPs. Trypsin can easily hydrolyze protamine, leading to the quench of the fluorescence. Then, the fluorescence could be recovered again by the addition of parathion-methyl (PM) which could inhibit the activity of trypsin. By measuring the fluorescence of RF-QDs, the inhibition efficiency of PM to trypsin activity was evaluated. Under the optimized conditions, the inhibition efficiency was proportional to the logarithm of PM concentration in the range of 0.04-400 ng mL(-1), with a detection limit of 0.018 ng mL(-1). Furthermore, the simple and convenient method had been used for PM detection in environmental and agricultural samples with satisfactory results. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/26143468/A_ratiometric_fluorescent_quantum_dots_based_biosensor_for_organophosphorus_pesticides_detection_by_inner_filter_effect_ DB - PRIME DP - Unbound Medicine ER -