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Proteomic profile response of Paracoccidioides lutzii to the antifungal argentilactone.
Front Microbiol. 2015; 6:616.FM

Abstract

The dimorphic fungi Paracoccidioides spp. are the etiological agents of paracoccidioidomycosis (PCM), a mycosis of high incidence in Brazil. The toxicity of drug treatment and the emergence of resistant organisms have led to research for new candidates for drugs. In this study, we demonstrate that the natural product argentilactone was not cytotoxic or genotoxic to MRC5 cells at the IC50 concentration to the fungus. We also verified the proteomic profile of Paracoccidioides lutzii after incubation with argentilactone using a label free quantitative proteome nanoUPLC-MS(E). The results of this study indicated that the fungus has a global metabolic adaptation in the presence of argentilactone. Enzymes of important pathways, such as glycolysis, the Krebs cycle and the glyoxylate cycle, were repressed, which drove the metabolism to the methylcytrate cycle and beta-oxidation. Proteins involved in cell rescue, defense and stress response were induced. In this study, alternative metabolic pathways adopted by the fungi were elucidated, helping to elucidate the course of action of the compound studied.

Authors+Show Affiliations

Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás Goiânia, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás Goiânia, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás Goiânia, Brazil.Laboratório de Produtos Naturais, Instituto de Química, Universidade Federal de Goiás Goiânia, Brazil.Laboratório de Produtos Naturais, Instituto de Química, Universidade Federal de Goiás Goiânia, Brazil.Laboratório de Espectrometria de Massa (PBI), Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia, Empresa Brasileira de Pesquisa Agropecuária Brasília, Brazil.Laboratório de Genética Molecular e Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal de Goiás Goiânia, Brazil.Laboratório de Genética Molecular e Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal de Goiás Goiânia, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás Goiânia, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás Goiânia, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26150808

Citation

Prado, Renata S., et al. "Proteomic Profile Response of Paracoccidioides Lutzii to the Antifungal Argentilactone." Frontiers in Microbiology, vol. 6, 2015, p. 616.
Prado RS, Bailão AM, Silva LC, et al. Proteomic profile response of Paracoccidioides lutzii to the antifungal argentilactone. Front Microbiol. 2015;6:616.
Prado, R. S., Bailão, A. M., Silva, L. C., de Oliveira, C. M., Marques, M. F., Silva, L. P., Silveira-Lacerda, E. P., Lima, A. P., Soares, C. M., & Pereira, M. (2015). Proteomic profile response of Paracoccidioides lutzii to the antifungal argentilactone. Frontiers in Microbiology, 6, 616. https://doi.org/10.3389/fmicb.2015.00616
Prado RS, et al. Proteomic Profile Response of Paracoccidioides Lutzii to the Antifungal Argentilactone. Front Microbiol. 2015;6:616. PubMed PMID: 26150808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic profile response of Paracoccidioides lutzii to the antifungal argentilactone. AU - Prado,Renata S, AU - Bailão,Alexandre M, AU - Silva,Lívia C, AU - de Oliveira,Cecília M A, AU - Marques,Monique F, AU - Silva,Luciano P, AU - Silveira-Lacerda,Elisângela P, AU - Lima,Aliny P, AU - Soares,Célia M, AU - Pereira,Maristela, Y1 - 2015/06/18/ PY - 2015/04/03/received PY - 2015/06/03/accepted PY - 2015/7/8/entrez PY - 2015/7/8/pubmed PY - 2015/7/8/medline KW - Paracoccidioides lutzzi KW - antifungal KW - argentilactone KW - paracoccidioidomycosis KW - proteomic SP - 616 EP - 616 JF - Frontiers in microbiology JO - Front Microbiol VL - 6 N2 - The dimorphic fungi Paracoccidioides spp. are the etiological agents of paracoccidioidomycosis (PCM), a mycosis of high incidence in Brazil. The toxicity of drug treatment and the emergence of resistant organisms have led to research for new candidates for drugs. In this study, we demonstrate that the natural product argentilactone was not cytotoxic or genotoxic to MRC5 cells at the IC50 concentration to the fungus. We also verified the proteomic profile of Paracoccidioides lutzii after incubation with argentilactone using a label free quantitative proteome nanoUPLC-MS(E). The results of this study indicated that the fungus has a global metabolic adaptation in the presence of argentilactone. Enzymes of important pathways, such as glycolysis, the Krebs cycle and the glyoxylate cycle, were repressed, which drove the metabolism to the methylcytrate cycle and beta-oxidation. Proteins involved in cell rescue, defense and stress response were induced. In this study, alternative metabolic pathways adopted by the fungi were elucidated, helping to elucidate the course of action of the compound studied. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/26150808/Proteomic_profile_response_of_Paracoccidioides_lutzii_to_the_antifungal_argentilactone_ L2 - https://doi.org/10.3389/fmicb.2015.00616 DB - PRIME DP - Unbound Medicine ER -
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