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Evaluation of anti-asthmatic and antioxidant potential of Boerhavia procumbens in toluene diisocyanate (TDI) treated rats.
J Ethnopharmacol. 2015 Aug 22; 172:377-85.JE

Abstract

AIM OF THE STUDY

Asthma is an ailment of airways characterized by activation of the T helper (Th) 2 lymphocytes and subsequent movement of inflammatory cells. Boerhavia procumbens of family Nyctaginaceae is locally used for the treatment of asthma, cough, hemorrhoids, dropsy, cardiac, eyes and kidney problems. We have evaluated its methanol extract (BPM) as a therapeutic candidate for asthma against toluene diisocyanate (TDI) allergic model in rat. The BPM extract was obtained from the whole plant of B. procumbens in methanol. Sprague-Dawley male 36 rats (200-250 g) were categorized into 6 groups having six rats in each category. The animals were provoked (10%) and sensitized (5%) by TDI. Animals of groups I-III were vehicle control (ethyl acetate), diseased control (TDI) and reference control (TDI+dexamethasone {2.5mg/kg bw}), respectively. Animals of group IV (TDI+200mg/kg bw) and group V (TDI+400mg/kg bw) were administered with BPM whereas group VI was administered with 400mg/kg bw alone of BPM. Protective effects of BPM were determined by counting the number of leucocytes and estimation of interleukines in blood, bronchoalveolar lavage (BAL) and in in vitro culture of spleen cells. Estimation of antioxidant enzymes, lipid peroxides and H2O2 and histopathology of lungs were carried out for antioxidant potential of plant extract used.

RESULTS

Methanol extract of B. procumbens suppressed the asthmatic symptoms and inhibited the infiltration of eosinophils and lymphocytes in lungs of TDI provoked rats. Administration of BPM to TDI provoked rats, dose dependently, inhibited the release of interleukins (IL)-2 in serum and IL-4, IL-6 interferon gamma (IFN-γ) in bronchoalveolar lavage (BAL) and in in vitro culture of spleen cells, and ameliorated the oxidative stress in lung tissues. Quantitative scoring of the lung histopathology exhibited protective effects of BPM and the inflammation, mucus, thickening of peribronchial smooth muscle layer and subepithelial deposition of collagen induced with TDI were ameliorated. The BPM has the anti-inflammatory properties that may be used to treat the asthma and inflammatory related ailments.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan. Electronic address: mrkhanqau@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26151242

Citation

Bokhari, Jasia, and Muhammad Rashid Khan. "Evaluation of Anti-asthmatic and Antioxidant Potential of Boerhavia Procumbens in Toluene Diisocyanate (TDI) Treated Rats." Journal of Ethnopharmacology, vol. 172, 2015, pp. 377-85.
Bokhari J, Khan MR. Evaluation of anti-asthmatic and antioxidant potential of Boerhavia procumbens in toluene diisocyanate (TDI) treated rats. J Ethnopharmacol. 2015;172:377-85.
Bokhari, J., & Khan, M. R. (2015). Evaluation of anti-asthmatic and antioxidant potential of Boerhavia procumbens in toluene diisocyanate (TDI) treated rats. Journal of Ethnopharmacology, 172, 377-85. https://doi.org/10.1016/j.jep.2015.06.049
Bokhari J, Khan MR. Evaluation of Anti-asthmatic and Antioxidant Potential of Boerhavia Procumbens in Toluene Diisocyanate (TDI) Treated Rats. J Ethnopharmacol. 2015 Aug 22;172:377-85. PubMed PMID: 26151242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of anti-asthmatic and antioxidant potential of Boerhavia procumbens in toluene diisocyanate (TDI) treated rats. AU - Bokhari,Jasia, AU - Khan,Muhammad Rashid, Y1 - 2015/07/04/ PY - 2015/02/26/received PY - 2015/06/22/revised PY - 2015/06/29/accepted PY - 2015/7/8/entrez PY - 2015/7/8/pubmed PY - 2016/6/2/medline KW - Asthma KW - Boerhavia procumbens KW - Eosinophils KW - Interleukins KW - Toluene diisocyanate KW - anti-Inflammatory SP - 377 EP - 85 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 172 N2 - AIM OF THE STUDY: Asthma is an ailment of airways characterized by activation of the T helper (Th) 2 lymphocytes and subsequent movement of inflammatory cells. Boerhavia procumbens of family Nyctaginaceae is locally used for the treatment of asthma, cough, hemorrhoids, dropsy, cardiac, eyes and kidney problems. We have evaluated its methanol extract (BPM) as a therapeutic candidate for asthma against toluene diisocyanate (TDI) allergic model in rat. The BPM extract was obtained from the whole plant of B. procumbens in methanol. Sprague-Dawley male 36 rats (200-250 g) were categorized into 6 groups having six rats in each category. The animals were provoked (10%) and sensitized (5%) by TDI. Animals of groups I-III were vehicle control (ethyl acetate), diseased control (TDI) and reference control (TDI+dexamethasone {2.5mg/kg bw}), respectively. Animals of group IV (TDI+200mg/kg bw) and group V (TDI+400mg/kg bw) were administered with BPM whereas group VI was administered with 400mg/kg bw alone of BPM. Protective effects of BPM were determined by counting the number of leucocytes and estimation of interleukines in blood, bronchoalveolar lavage (BAL) and in in vitro culture of spleen cells. Estimation of antioxidant enzymes, lipid peroxides and H2O2 and histopathology of lungs were carried out for antioxidant potential of plant extract used. RESULTS: Methanol extract of B. procumbens suppressed the asthmatic symptoms and inhibited the infiltration of eosinophils and lymphocytes in lungs of TDI provoked rats. Administration of BPM to TDI provoked rats, dose dependently, inhibited the release of interleukins (IL)-2 in serum and IL-4, IL-6 interferon gamma (IFN-γ) in bronchoalveolar lavage (BAL) and in in vitro culture of spleen cells, and ameliorated the oxidative stress in lung tissues. Quantitative scoring of the lung histopathology exhibited protective effects of BPM and the inflammation, mucus, thickening of peribronchial smooth muscle layer and subepithelial deposition of collagen induced with TDI were ameliorated. The BPM has the anti-inflammatory properties that may be used to treat the asthma and inflammatory related ailments. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26151242/Evaluation_of_anti_asthmatic_and_antioxidant_potential_of_Boerhavia_procumbens_in_toluene_diisocyanate__TDI__treated_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)30014-3 DB - PRIME DP - Unbound Medicine ER -