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DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters.
Fertil Steril 2015; 104(3):582-90.e4FS

Abstract

OBJECTIVE

To evaluate whether sperm DNA fragmentation (sDF), measured in brighter, dimmer, and total populations, predicts natural conception, and to evaluate the intra-individual variability of sDF.

DESIGN

Prospective study.

SETTING

Outpatient clinic and diagnostic laboratory.

PATIENT(S)

A total of 348 unselected patients and 86 proven fertile men.

INTERVENTION(S)

None.

MAIN OUTCOME MEASURE(S)

sDF was revealed with the use of terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL)/propidium iodide (PI). Receiver operating characteristic (ROC) curves were built before and after matching fertile men to patients for age (76:152) or semen parameters (68:136) or both (49:98). Intra-individual variability of sDF was assessed over 2 years.

RESULT(S)

Brighter (area under ROC curve [AUC] 0.718 ± 0.54), dimmer (AUC 0.655 ± 0.63), and total (AUC 0.757 ± 0.54) sDF predict male fertility in unmatched and age- or semen parameters-matched subjects. After matching for both age and semen parameters, only brighter (AUC 0.711 ± 0.83) and total (AUC 0.675 ± 0.92) sDF predict male fertility. At high values of total sDF, brighter predicts natural conception better than total sDF. Intra-individual coefficients of variation of sDF were 9.2 ± 8.6% (n = 25), 12.9 ± 12.7% (n = 53), and 14.0 ± 12.6% (n = 70) over, respectively, 100-day and 1- and 2-year periods, appearing to be the most stable of the evaluated semen parameters.

CONCLUSION(S)

The predictive power of total sDF partially depends on age and semen parameters, whereas brighter sDF independently predicts natural conception. Therefore, brighter sDF is a fraction of sDF that adds new information to the routine semen analysis. At high levels of sDF, distinguishing the two sperm populations improves the predictive power of sDF. Overall, our results support the idea that TUNEL/PI can be of clinical usefulness in the male fertility workup.

Authors+Show Affiliations

Department of Experimental, Clinical and Biomedical Sciences, DeNoth Center of Excellence, University of Florence, Florence, Italy. Electronic address: monica.muratori@unifi.it.Department of Experimental, Clinical and Biomedical Sciences, DeNoth Center of Excellence, University of Florence, Florence, Italy.Department of Experimental, Clinical and Biomedical Sciences, DeNoth Center of Excellence, University of Florence, Florence, Italy.Department of Experimental, Clinical and Biomedical Sciences, DeNoth Center of Excellence, University of Florence, Florence, Italy.Department of Experimental, Clinical and Biomedical Sciences, DeNoth Center of Excellence, University of Florence, Florence, Italy.Seminology Laboratory, Azienda USL3 Pistoia, Pistoia, Italy.Azienda Ospedaliero-Universitaria Careggi, Florence, Italy.Department of Experimental, Clinical and Biomedical Sciences, DeNoth Center of Excellence, University of Florence, Florence, Italy.Department of Experimental, Clinical and Biomedical Sciences, DeNoth Center of Excellence, University of Florence, Florence, Italy.Department of Experimental, Clinical and Biomedical Sciences, DeNoth Center of Excellence, University of Florence, Florence, Italy.Department of Experimental, Clinical and Biomedical Sciences, DeNoth Center of Excellence, University of Florence, Florence, Italy.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26151619

Citation

Muratori, Monica, et al. "DNA Fragmentation in Brighter Sperm Predicts Male Fertility Independently From Age and Semen Parameters." Fertility and Sterility, vol. 104, no. 3, 2015, pp. 582-90.e4.
Muratori M, Marchiani S, Tamburrino L, et al. DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters. Fertil Steril. 2015;104(3):582-90.e4.
Muratori, M., Marchiani, S., Tamburrino, L., Cambi, M., Lotti, F., Natali, I., ... Baldi, E. (2015). DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters. Fertility and Sterility, 104(3), pp. 582-90.e4. doi:10.1016/j.fertnstert.2015.06.005.
Muratori M, et al. DNA Fragmentation in Brighter Sperm Predicts Male Fertility Independently From Age and Semen Parameters. Fertil Steril. 2015;104(3):582-90.e4. PubMed PMID: 26151619.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters. AU - Muratori,Monica, AU - Marchiani,Sara, AU - Tamburrino,Lara, AU - Cambi,Marta, AU - Lotti,Francesco, AU - Natali,Ilaria, AU - Filimberti,Erminio, AU - Noci,Ivo, AU - Forti,Gianni, AU - Maggi,Mario, AU - Baldi,Elisabetta, Y1 - 2015/07/04/ PY - 2015/03/26/received PY - 2015/06/04/revised PY - 2015/06/04/accepted PY - 2015/7/8/entrez PY - 2015/7/8/pubmed PY - 2016/5/5/medline KW - Sperm DNA fragmentation KW - TUNEL/PI KW - male fertility KW - natural conception SP - 582 EP - 90.e4 JF - Fertility and sterility JO - Fertil. Steril. VL - 104 IS - 3 N2 - OBJECTIVE: To evaluate whether sperm DNA fragmentation (sDF), measured in brighter, dimmer, and total populations, predicts natural conception, and to evaluate the intra-individual variability of sDF. DESIGN: Prospective study. SETTING: Outpatient clinic and diagnostic laboratory. PATIENT(S): A total of 348 unselected patients and 86 proven fertile men. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): sDF was revealed with the use of terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL)/propidium iodide (PI). Receiver operating characteristic (ROC) curves were built before and after matching fertile men to patients for age (76:152) or semen parameters (68:136) or both (49:98). Intra-individual variability of sDF was assessed over 2 years. RESULT(S): Brighter (area under ROC curve [AUC] 0.718 ± 0.54), dimmer (AUC 0.655 ± 0.63), and total (AUC 0.757 ± 0.54) sDF predict male fertility in unmatched and age- or semen parameters-matched subjects. After matching for both age and semen parameters, only brighter (AUC 0.711 ± 0.83) and total (AUC 0.675 ± 0.92) sDF predict male fertility. At high values of total sDF, brighter predicts natural conception better than total sDF. Intra-individual coefficients of variation of sDF were 9.2 ± 8.6% (n = 25), 12.9 ± 12.7% (n = 53), and 14.0 ± 12.6% (n = 70) over, respectively, 100-day and 1- and 2-year periods, appearing to be the most stable of the evaluated semen parameters. CONCLUSION(S): The predictive power of total sDF partially depends on age and semen parameters, whereas brighter sDF independently predicts natural conception. Therefore, brighter sDF is a fraction of sDF that adds new information to the routine semen analysis. At high levels of sDF, distinguishing the two sperm populations improves the predictive power of sDF. Overall, our results support the idea that TUNEL/PI can be of clinical usefulness in the male fertility workup. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/26151619/DNA_fragmentation_in_brighter_sperm_predicts_male_fertility_independently_from_age_and_semen_parameters_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(15)00427-6 DB - PRIME DP - Unbound Medicine ER -