Tags

Type your tag names separated by a space and hit enter

Mesenchymal stromal cells for cutaneous wound healing in a rabbit model: pre-clinical study applicable in the pediatric surgical setting.
J Transl Med 2015; 13:219JT

Abstract

OBJECTIVE

Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs).

METHODS

Mesenchymal stromal cells were in vitro expanded from adipose and BM tissues of young female New Zealand rabbits. MSCs were characterized for plastic adhesion, surface markers, proliferation and differentiation capacity. When an adequate number of cells (ASCs 10 × 10(6) and BM-MSCs 3 × 10(6), because of their low rate of proliferation) was reached, two skin wounds were surgically induced in each animal. The first was topically treated with cell infusions, the second was used as a control. The intradermal inoculation included autologous or allogeneic ASCs or autologous BM-MSCs. For histological examination, animals were sacrificed and wounds were harvested after 11 and 21 days of treatment.

RESULTS

Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29). Topically, ASC inoculation provided more rapid wound healing than BM-MSCs and controls. Improved re-epithelization, reduced inflammatory infiltration and increased collagen deposition were observed in biopsies from wounds treated with ASCs, with the best result in the autologous setting. ASCs also improved restoration of skin architecture during wound healing.

CONCLUSION

The use of ASCs may offer a promising solution to treat extended wounds. Pre-clinical studies are however necessary to validate the best skin regeneration technique, which could be used in pediatric surgical translational research.

Authors+Show Affiliations

Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, 27100, Pavia, Italy. g.pelizzo@smatteo.pv.it.Immunology and Transplantation Laboratory/Cell Factory/Pediatric Hematology/Oncology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. ma.avanzini@smatteo.pv.it.Histology and Embryology Unit, Department of Public Health, Experimental Medicine and Forensic, University of Pavia, Pavia, Italy. antonia.icaro@unipv.it.Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, 27100, Pavia, Italy. hostim@hotmail.com.Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, 27100, Pavia, Italy. p.romano@smatteo.pv.it.Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, 27100, Pavia, Italy. l.avolio@smatteo.pv.it.Immunology and Transplantation Laboratory/Cell Factory/Pediatric Hematology/Oncology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. r.maccario@smatteo.pv.it.Laboratory of Cellular Therapies, Department of Medical and Surgical Sciences for Children and Adults, University Hospital of Modena and Reggio, Emilia, Italy. massimo.dominici@unimore.it.Biometry and Clinical Epidemiology Unit, Scientific Direction, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. a.desilvestri@smatteo.pv.it.Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, 27100, Pavia, Italy. erika.andreatta87@gmail.com.Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, 27100, Pavia, Italy. federico.costanzo01@gmail.com.Immunology and Transplantation Laboratory/Cell Factory/Pediatric Hematology/Oncology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. melissamantelli@tiscali.it.Immunology and Transplantation Laboratory/Cell Factory/Pediatric Hematology/Oncology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. daniela.ingo31@libero.it.Laboratory of Cellular Therapies, Department of Medical and Surgical Sciences for Children and Adults, University Hospital of Modena and Reggio, Emilia, Italy. mariaserena.piccinno@unimore.it.Pediatric Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy. v.calcaterra@smatteo.pv.it.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26152232

Citation

Pelizzo, Gloria, et al. "Mesenchymal Stromal Cells for Cutaneous Wound Healing in a Rabbit Model: Pre-clinical Study Applicable in the Pediatric Surgical Setting." Journal of Translational Medicine, vol. 13, 2015, p. 219.
Pelizzo G, Avanzini MA, Icaro Cornaglia A, et al. Mesenchymal stromal cells for cutaneous wound healing in a rabbit model: pre-clinical study applicable in the pediatric surgical setting. J Transl Med. 2015;13:219.
Pelizzo, G., Avanzini, M. A., Icaro Cornaglia, A., Osti, M., Romano, P., Avolio, L., ... Calcaterra, V. (2015). Mesenchymal stromal cells for cutaneous wound healing in a rabbit model: pre-clinical study applicable in the pediatric surgical setting. Journal of Translational Medicine, 13, p. 219. doi:10.1186/s12967-015-0580-3.
Pelizzo G, et al. Mesenchymal Stromal Cells for Cutaneous Wound Healing in a Rabbit Model: Pre-clinical Study Applicable in the Pediatric Surgical Setting. J Transl Med. 2015 Jul 8;13:219. PubMed PMID: 26152232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mesenchymal stromal cells for cutaneous wound healing in a rabbit model: pre-clinical study applicable in the pediatric surgical setting. AU - Pelizzo,Gloria, AU - Avanzini,Maria Antonietta, AU - Icaro Cornaglia,Antonia, AU - Osti,Monica, AU - Romano,Piero, AU - Avolio,Luigi, AU - Maccario,Rita, AU - Dominici,Massimo, AU - De Silvestri,Annalisa, AU - Andreatta,Erika, AU - Costanzo,Federico, AU - Mantelli,Melissa, AU - Ingo,Daniela, AU - Piccinno,Serena, AU - Calcaterra,Valeria, Y1 - 2015/07/08/ PY - 2015/05/11/received PY - 2015/06/24/accepted PY - 2015/7/9/entrez PY - 2015/7/15/pubmed PY - 2016/3/15/medline SP - 219 EP - 219 JF - Journal of translational medicine JO - J Transl Med VL - 13 N2 - OBJECTIVE: Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs). METHODS: Mesenchymal stromal cells were in vitro expanded from adipose and BM tissues of young female New Zealand rabbits. MSCs were characterized for plastic adhesion, surface markers, proliferation and differentiation capacity. When an adequate number of cells (ASCs 10 × 10(6) and BM-MSCs 3 × 10(6), because of their low rate of proliferation) was reached, two skin wounds were surgically induced in each animal. The first was topically treated with cell infusions, the second was used as a control. The intradermal inoculation included autologous or allogeneic ASCs or autologous BM-MSCs. For histological examination, animals were sacrificed and wounds were harvested after 11 and 21 days of treatment. RESULTS: Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29). Topically, ASC inoculation provided more rapid wound healing than BM-MSCs and controls. Improved re-epithelization, reduced inflammatory infiltration and increased collagen deposition were observed in biopsies from wounds treated with ASCs, with the best result in the autologous setting. ASCs also improved restoration of skin architecture during wound healing. CONCLUSION: The use of ASCs may offer a promising solution to treat extended wounds. Pre-clinical studies are however necessary to validate the best skin regeneration technique, which could be used in pediatric surgical translational research. SN - 1479-5876 UR - https://www.unboundmedicine.com/medline/citation/26152232/Mesenchymal_stromal_cells_for_cutaneous_wound_healing_in_a_rabbit_model:_pre_clinical_study_applicable_in_the_pediatric_surgical_setting_ L2 - https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-015-0580-3 DB - PRIME DP - Unbound Medicine ER -