Tags

Type your tag names separated by a space and hit enter

Detection of BRAF c.1799T > A (p.V600E) mutation using residual routine fine-needle aspiration specimens of papillary thyroid carcinoma.
Diagn Cytopathol. 2015 Oct; 43(10):786-90.DC

Abstract

BACKGROUND

BRAF p.V600E mutation is the most common genetic alteration in papillary thyroid cancer (PTC) and has been used as a diagnostic and prognostic marker in PTC. The aim of this study was to investigate the utility of preoperative BRAF p.V600E mutation analysis as an adjunctive diagnostic and prognostic tool to routine fine-needle aspiration (FNA).

METHODS

Specimens were collected from thyroid nodules by FNA. Cytology diagnosis and BRAF p.V600E testing were performed on these specimens. Molecular and cytological results were correlated with histology outcomes.

RESULTS

A total of 195 patients with thyroid nodules were enrolled, including 25 benign lesions and 170 PTCs. BRAF p.V600E testing was successfully performed in all specimens. The combination of BRAF p.V600E testing and cytology improved the sensitivity of cytology from 70% to 85.3% (P = 0.001). This significant increase in sensitivity was due to the detection of PTC by BRAF p.V600E testing in the nodules with atypical or suspicious PTC cytology results. Patients with BRAF p.V600E-positive tumors were significantly older than those who did not harbor mutations (45.6 years vs. 39.8 years, P = 0.002). No correlations between BRAF p.V600E mutation and other clinical-pathology parameters were observed.

CONCLUSIONS

Detection of BRAF p.V600E mutation can be successfully carried out using residual liquid-based materials. It can be performed as a diagnostic tool to supplement traditional thyroid FNA, especially in cases with atypical or suspicious PTC. However, the role of BRAF p.V600E in guidance of the extent of thyroidectomy and nodal clearance requires further study.

Authors+Show Affiliations

Department of Pathology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, People's Republic of China.Department of Pathology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, People's Republic of China.Department of Pathology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, People's Republic of China.State Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, People's Republic of China.Department of Pathology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, People's Republic of China.Department of Pathology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26152656

Citation

Zhao, Huan, et al. "Detection of BRAF c.1799T > a (p.V600E) Mutation Using Residual Routine Fine-needle Aspiration Specimens of Papillary Thyroid Carcinoma." Diagnostic Cytopathology, vol. 43, no. 10, 2015, pp. 786-90.
Zhao H, Zhang ZH, Zhou B, et al. Detection of BRAF c.1799T > A (p.V600E) mutation using residual routine fine-needle aspiration specimens of papillary thyroid carcinoma. Diagn Cytopathol. 2015;43(10):786-90.
Zhao, H., Zhang, Z. H., Zhou, B., Xiao, T., Pan, Q. J., & Guo, H. Q. (2015). Detection of BRAF c.1799T > A (p.V600E) mutation using residual routine fine-needle aspiration specimens of papillary thyroid carcinoma. Diagnostic Cytopathology, 43(10), 786-90. https://doi.org/10.1002/dc.23302
Zhao H, et al. Detection of BRAF c.1799T > a (p.V600E) Mutation Using Residual Routine Fine-needle Aspiration Specimens of Papillary Thyroid Carcinoma. Diagn Cytopathol. 2015;43(10):786-90. PubMed PMID: 26152656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of BRAF c.1799T > A (p.V600E) mutation using residual routine fine-needle aspiration specimens of papillary thyroid carcinoma. AU - Zhao,Huan, AU - Zhang,Zhi-hui, AU - Zhou,Bin, AU - Xiao,Ting, AU - Pan,Qin-jing, AU - Guo,Hui-qin, Y1 - 2015/07/07/ PY - 2014/06/25/received PY - 2015/04/10/revised PY - 2015/06/19/accepted PY - 2015/7/9/entrez PY - 2015/7/15/pubmed PY - 2016/7/1/medline KW - BRAF p.V600E mutation KW - diagnosis KW - fine-needle aspiration KW - papillary thyroid carcinoma KW - prognosis SP - 786 EP - 90 JF - Diagnostic cytopathology JO - Diagn. Cytopathol. VL - 43 IS - 10 N2 - BACKGROUND: BRAF p.V600E mutation is the most common genetic alteration in papillary thyroid cancer (PTC) and has been used as a diagnostic and prognostic marker in PTC. The aim of this study was to investigate the utility of preoperative BRAF p.V600E mutation analysis as an adjunctive diagnostic and prognostic tool to routine fine-needle aspiration (FNA). METHODS: Specimens were collected from thyroid nodules by FNA. Cytology diagnosis and BRAF p.V600E testing were performed on these specimens. Molecular and cytological results were correlated with histology outcomes. RESULTS: A total of 195 patients with thyroid nodules were enrolled, including 25 benign lesions and 170 PTCs. BRAF p.V600E testing was successfully performed in all specimens. The combination of BRAF p.V600E testing and cytology improved the sensitivity of cytology from 70% to 85.3% (P = 0.001). This significant increase in sensitivity was due to the detection of PTC by BRAF p.V600E testing in the nodules with atypical or suspicious PTC cytology results. Patients with BRAF p.V600E-positive tumors were significantly older than those who did not harbor mutations (45.6 years vs. 39.8 years, P = 0.002). No correlations between BRAF p.V600E mutation and other clinical-pathology parameters were observed. CONCLUSIONS: Detection of BRAF p.V600E mutation can be successfully carried out using residual liquid-based materials. It can be performed as a diagnostic tool to supplement traditional thyroid FNA, especially in cases with atypical or suspicious PTC. However, the role of BRAF p.V600E in guidance of the extent of thyroidectomy and nodal clearance requires further study. SN - 1097-0339 UR - https://www.unboundmedicine.com/medline/citation/26152656/Detection_of_BRAF_c_1799T_>_A__p_V600E__mutation_using_residual_routine_fine_needle_aspiration_specimens_of_papillary_thyroid_carcinoma_ L2 - https://doi.org/10.1002/dc.23302 DB - PRIME DP - Unbound Medicine ER -