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Development of tyrosinase biosensor based on quantum dots/chitosan nanocomposite for detection of phenolic compounds.
Anal Biochem. 2015 Oct 01; 486:102-6.AB

Abstract

A sensitive and simple amperometric biosensor for phenols was developed based on the immobilization of tyrosinase into CdS quantum dots/chitosan nanocomposite matrix. The nanocomposite film with porous nanostructure, excellent hydrophilicity and biocompatibility resulted in high enzyme loading, and the tyrosinase (Tyr) immobilized in this novel matrix retained its activity to a large extent. The CdS quantum dots/chitosan nanocomposite film was characterized by scanning electron microscopy and electrochemical impedance spectroscopy, and the parameters of the various experimental variables for the biosensor were optimized. Under the optimal conditions, the designed biosensor displayed a wide linear response to catechol over a concentration range of 1.0×10(-9) to 2.0×10(-5)M with a high sensitivity of 561±9.7mAM(-1) and a low detection limit down to 0.3 nM at a signal-to-noise ratio of 3. The CdS quantum dots/chitosan nanocomposites could provide a novel matrix for enzyme immobilization to promote the development of biosensing and biocatalysis.

Authors+Show Affiliations

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China. Electronic address: enhan182@163.com.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.Key Laboratory of Modern Agricultural Equipment and Technology (MOE), Institute of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26159737

Citation

Han, En, et al. "Development of Tyrosinase Biosensor Based On Quantum Dots/chitosan Nanocomposite for Detection of Phenolic Compounds." Analytical Biochemistry, vol. 486, 2015, pp. 102-6.
Han E, Yang Y, He Z, et al. Development of tyrosinase biosensor based on quantum dots/chitosan nanocomposite for detection of phenolic compounds. Anal Biochem. 2015;486:102-6.
Han, E., Yang, Y., He, Z., Cai, J., Zhang, X., & Dong, X. (2015). Development of tyrosinase biosensor based on quantum dots/chitosan nanocomposite for detection of phenolic compounds. Analytical Biochemistry, 486, 102-6. https://doi.org/10.1016/j.ab.2015.07.001
Han E, et al. Development of Tyrosinase Biosensor Based On Quantum Dots/chitosan Nanocomposite for Detection of Phenolic Compounds. Anal Biochem. 2015 Oct 1;486:102-6. PubMed PMID: 26159737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of tyrosinase biosensor based on quantum dots/chitosan nanocomposite for detection of phenolic compounds. AU - Han,En, AU - Yang,Yi, AU - He,Zheng, AU - Cai,Jianrong, AU - Zhang,Xinai, AU - Dong,Xiaoya, Y1 - 2015/07/06/ PY - 2015/04/26/received PY - 2015/06/30/revised PY - 2015/07/01/accepted PY - 2015/7/11/entrez PY - 2015/7/15/pubmed PY - 2016/5/14/medline KW - Amperometric detection KW - Nanocomposite KW - Phenolic compounds KW - Quantum dots SP - 102 EP - 6 JF - Analytical biochemistry JO - Anal Biochem VL - 486 N2 - A sensitive and simple amperometric biosensor for phenols was developed based on the immobilization of tyrosinase into CdS quantum dots/chitosan nanocomposite matrix. The nanocomposite film with porous nanostructure, excellent hydrophilicity and biocompatibility resulted in high enzyme loading, and the tyrosinase (Tyr) immobilized in this novel matrix retained its activity to a large extent. The CdS quantum dots/chitosan nanocomposite film was characterized by scanning electron microscopy and electrochemical impedance spectroscopy, and the parameters of the various experimental variables for the biosensor were optimized. Under the optimal conditions, the designed biosensor displayed a wide linear response to catechol over a concentration range of 1.0×10(-9) to 2.0×10(-5)M with a high sensitivity of 561±9.7mAM(-1) and a low detection limit down to 0.3 nM at a signal-to-noise ratio of 3. The CdS quantum dots/chitosan nanocomposites could provide a novel matrix for enzyme immobilization to promote the development of biosensing and biocatalysis. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/26159737/Development_of_tyrosinase_biosensor_based_on_quantum_dots/chitosan_nanocomposite_for_detection_of_phenolic_compounds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(15)00339-5 DB - PRIME DP - Unbound Medicine ER -