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Fine mapping of powdery mildew resistance genes PmTb7A.1 and PmTb7A.2 in Triticum boeoticum (Boiss.) using the shotgun sequence assembly of chromosome 7AL.
Theor Appl Genet. 2015 Oct; 128(10):2099-111.TA

Abstract

KEY MESSAGE

A novel powdery mildew resistance gene and a new allele of Pm1 were identified and fine mapped. DNA markers suitable for marker-assisted selection have been identified. Powdery mildew caused by Blumeria graminis is one of the most important foliar diseases of wheat and causes significant yield losses worldwide. Diploid A genome species are an important genetic resource for disease resistance genes. Two powdery mildew resistance genes, identified in Triticum boeoticum (A(b)A(b)) accession pau5088, PmTb7A.1 and PmTb7A.2 were mapped on chromosome 7AL. In the present study, shotgun sequence assembly data for chromosome 7AL were utilised for fine mapping of these Pm resistance genes. Forty SSR, 73 resistance gene analogue-based sequence-tagged sites (RGA-STS) and 36 single nucleotide polymorphism markers were designed for fine mapping of PmTb7A.1 and PmTb7A.2. Twenty-one RGA-STS, 8 SSR and 13 SNP markers were mapped to 7AL. RGA-STS markers Ta7AL-4556232 and 7AL-4426363 were linked to the PmTb7A.1 and PmTb7A.2, at a genetic distance of 0.6 and 6.0 cM, respectively. The present investigation established that PmTb7A.1 is a new powdery mildew resistance gene that confers resistance to a broad range of Bgt isolates, whereas PmTb7A.2 most probably is a new allele of Pm1 based on chromosomal location and screening with Bgt isolates showing differential reaction on lines with different Pm1 alleles. The markers identified to be linked to the two Pm resistance genes are robust and can be used for marker-assisted introgression of these genes to hexaploid wheat.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Chhuneja P
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Yadav B
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Stirnweis D
Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
Hurni S
Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
Kaur S
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Elkot AF
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India. Wheat Research Department, Field Crops Research Institute, Agriculture Research Center, Giza, 12619, Egypt.
Keller B
Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
Wicker T
Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
Sehgal S
Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA. Department of Plant Science, South Dakota State University, Brookings, 57007, USA.
Gill BS
Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.
Singh K
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India. kuldeep35@pau.edu.

MeSH

AllelesAscomycotaChromosome MappingChromosomes, PlantDiploidyDisease ResistanceGenes, PlantGenetic LinkageGenetic MarkersPlant BreedingPlant DiseasesPolymorphism, Single NucleotideSequence Tagged SitesTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26160336

Citation

Chhuneja, Parveen, et al. "Fine Mapping of Powdery Mildew Resistance Genes PmTb7A.1 and PmTb7A.2 in Triticum Boeoticum (Boiss.) Using the Shotgun Sequence Assembly of Chromosome 7AL." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 128, no. 10, 2015, pp. 2099-111.
Chhuneja P, Yadav B, Stirnweis D, et al. Fine mapping of powdery mildew resistance genes PmTb7A.1 and PmTb7A.2 in Triticum boeoticum (Boiss.) using the shotgun sequence assembly of chromosome 7AL. Theor Appl Genet. 2015;128(10):2099-111.
Chhuneja, P., Yadav, B., Stirnweis, D., Hurni, S., Kaur, S., Elkot, A. F., Keller, B., Wicker, T., Sehgal, S., Gill, B. S., & Singh, K. (2015). Fine mapping of powdery mildew resistance genes PmTb7A.1 and PmTb7A.2 in Triticum boeoticum (Boiss.) using the shotgun sequence assembly of chromosome 7AL. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 128(10), 2099-111. https://doi.org/10.1007/s00122-015-2570-5
Chhuneja P, et al. Fine Mapping of Powdery Mildew Resistance Genes PmTb7A.1 and PmTb7A.2 in Triticum Boeoticum (Boiss.) Using the Shotgun Sequence Assembly of Chromosome 7AL. Theor Appl Genet. 2015;128(10):2099-111. PubMed PMID: 26160336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fine mapping of powdery mildew resistance genes PmTb7A.1 and PmTb7A.2 in Triticum boeoticum (Boiss.) using the shotgun sequence assembly of chromosome 7AL. AU - Chhuneja,Parveen, AU - Yadav,Bharat, AU - Stirnweis,Daniel, AU - Hurni,Severine, AU - Kaur,Satinder, AU - Elkot,Ahmed Fawzy, AU - Keller,Beat, AU - Wicker,Thomas, AU - Sehgal,Sunish, AU - Gill,Bikram S, AU - Singh,Kuldeep, Y1 - 2015/07/10/ PY - 2015/04/04/received PY - 2015/06/19/accepted PY - 2015/7/11/entrez PY - 2015/7/15/pubmed PY - 2015/12/24/medline SP - 2099 EP - 111 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 128 IS - 10 N2 - KEY MESSAGE: A novel powdery mildew resistance gene and a new allele of Pm1 were identified and fine mapped. DNA markers suitable for marker-assisted selection have been identified. Powdery mildew caused by Blumeria graminis is one of the most important foliar diseases of wheat and causes significant yield losses worldwide. Diploid A genome species are an important genetic resource for disease resistance genes. Two powdery mildew resistance genes, identified in Triticum boeoticum (A(b)A(b)) accession pau5088, PmTb7A.1 and PmTb7A.2 were mapped on chromosome 7AL. In the present study, shotgun sequence assembly data for chromosome 7AL were utilised for fine mapping of these Pm resistance genes. Forty SSR, 73 resistance gene analogue-based sequence-tagged sites (RGA-STS) and 36 single nucleotide polymorphism markers were designed for fine mapping of PmTb7A.1 and PmTb7A.2. Twenty-one RGA-STS, 8 SSR and 13 SNP markers were mapped to 7AL. RGA-STS markers Ta7AL-4556232 and 7AL-4426363 were linked to the PmTb7A.1 and PmTb7A.2, at a genetic distance of 0.6 and 6.0 cM, respectively. The present investigation established that PmTb7A.1 is a new powdery mildew resistance gene that confers resistance to a broad range of Bgt isolates, whereas PmTb7A.2 most probably is a new allele of Pm1 based on chromosomal location and screening with Bgt isolates showing differential reaction on lines with different Pm1 alleles. The markers identified to be linked to the two Pm resistance genes are robust and can be used for marker-assisted introgression of these genes to hexaploid wheat. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/26160336/Fine_mapping_of_powdery_mildew_resistance_genes_PmTb7A_1_and_PmTb7A_2_in_Triticum_boeoticum__Boiss___using_the_shotgun_sequence_assembly_of_chromosome_7AL_ DB - PRIME DP - Unbound Medicine ER -