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Comparison of the early host immune response to two widely diverse virulent strains of Burkholderia pseudomallei that cause acute or chronic infections in BALB/c mice.
Microb Pathog. 2015 Sep; 86:53-63.MP

Abstract

Burkholderia pseudomallei is the etiologic agent of melioidosis, which is endemic in Southeast Asia and Northern Australia. We previously found by the intraperitoneal (IP) route that we could discern differences in virulence in mice amongst different strains of B. pseudomallei. We report an early immune response study comparing two strains in our collection which represent the least, B. pseudomallei 1106a, and one of the most, HBPUB10134a, virulent strains in BALB/c mice. B. pseudomallei HBPUB10134a infected mouse spleens contained a 2-3 log higher bacterial burden than mice infected with B. pseudomallei 1106a 3 days post-infection (PI). More and higher amounts of inflammatory cytokines/chemokines were detected in sera and spleen extracts from B. pseudomallei HBPUB10134a than B. pseudomallei 1106a infected mice. The most prominent were IFNγ, IL-1α, IL-1β, IL-6, IL-10, IP-10, and MIG. After 7 days PI, there was a decrease in bacterial burden in spleens from 1106a infected mice and a decrease in cytokines/chemokines in sera and spleen extracts from both sets of mice. By day 14 PI we saw an increase in monocytes/macrophages, NK cells, and granulocytes in spleens from both sets of mice. No B. pseudomallei HBPUB10134a infected mice survived after this time. In summary, B. pseudomallei HBPUB10134a was more virulent and induced host innate immune responses typical of a more acute-type infection than did B. pseudomallei 1106a which produced a more chronic infection in mice.

Authors+Show Affiliations

United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Bacteriology Division, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA. Electronic address: kei.amemiya.civ@mail.mil.United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Bacteriology Division, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Bacteriology Division, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Bacteriology Division, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Bacteriology Division, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Bacteriology Division, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26162294

Citation

Amemiya, Kei, et al. "Comparison of the Early Host Immune Response to Two Widely Diverse Virulent Strains of Burkholderia Pseudomallei That Cause Acute or Chronic Infections in BALB/c Mice." Microbial Pathogenesis, vol. 86, 2015, pp. 53-63.
Amemiya K, Dankmeyer JL, Fetterer DP, et al. Comparison of the early host immune response to two widely diverse virulent strains of Burkholderia pseudomallei that cause acute or chronic infections in BALB/c mice. Microb Pathog. 2015;86:53-63.
Amemiya, K., Dankmeyer, J. L., Fetterer, D. P., Worsham, P. L., Welkos, S. L., & Cote, C. K. (2015). Comparison of the early host immune response to two widely diverse virulent strains of Burkholderia pseudomallei that cause acute or chronic infections in BALB/c mice. Microbial Pathogenesis, 86, 53-63. https://doi.org/10.1016/j.micpath.2015.07.004
Amemiya K, et al. Comparison of the Early Host Immune Response to Two Widely Diverse Virulent Strains of Burkholderia Pseudomallei That Cause Acute or Chronic Infections in BALB/c Mice. Microb Pathog. 2015;86:53-63. PubMed PMID: 26162294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the early host immune response to two widely diverse virulent strains of Burkholderia pseudomallei that cause acute or chronic infections in BALB/c mice. AU - Amemiya,Kei, AU - Dankmeyer,Jennifer L, AU - Fetterer,David P, AU - Worsham,Patricia L, AU - Welkos,Susan L, AU - Cote,Christopher K, Y1 - 2015/07/07/ PY - 2015/04/06/received PY - 2015/06/30/revised PY - 2015/07/02/accepted PY - 2015/7/12/entrez PY - 2015/7/15/pubmed PY - 2016/5/5/medline KW - Acute infection KW - Burkholderia pseudomallei KW - Chronic infection KW - Immune response KW - Melioidosis SP - 53 EP - 63 JF - Microbial pathogenesis JO - Microb Pathog VL - 86 N2 - Burkholderia pseudomallei is the etiologic agent of melioidosis, which is endemic in Southeast Asia and Northern Australia. We previously found by the intraperitoneal (IP) route that we could discern differences in virulence in mice amongst different strains of B. pseudomallei. We report an early immune response study comparing two strains in our collection which represent the least, B. pseudomallei 1106a, and one of the most, HBPUB10134a, virulent strains in BALB/c mice. B. pseudomallei HBPUB10134a infected mouse spleens contained a 2-3 log higher bacterial burden than mice infected with B. pseudomallei 1106a 3 days post-infection (PI). More and higher amounts of inflammatory cytokines/chemokines were detected in sera and spleen extracts from B. pseudomallei HBPUB10134a than B. pseudomallei 1106a infected mice. The most prominent were IFNγ, IL-1α, IL-1β, IL-6, IL-10, IP-10, and MIG. After 7 days PI, there was a decrease in bacterial burden in spleens from 1106a infected mice and a decrease in cytokines/chemokines in sera and spleen extracts from both sets of mice. By day 14 PI we saw an increase in monocytes/macrophages, NK cells, and granulocytes in spleens from both sets of mice. No B. pseudomallei HBPUB10134a infected mice survived after this time. In summary, B. pseudomallei HBPUB10134a was more virulent and induced host innate immune responses typical of a more acute-type infection than did B. pseudomallei 1106a which produced a more chronic infection in mice. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/26162294/Comparison_of_the_early_host_immune_response_to_two_widely_diverse_virulent_strains_of_Burkholderia_pseudomallei_that_cause_acute_or_chronic_infections_in_BALB/c_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(15)00104-7 DB - PRIME DP - Unbound Medicine ER -