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Simple determination of fluoride in biological samples by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry.
J Chromatogr A. 2015 Aug 14; 1407:216-21.JC

Abstract

A simple and convenient method to detect fluoride in biological samples was developed. This method was based on derivatization with 2-(bromomethyl)naphthalene, headspace solid phase microextraction (HS-SPME) in a vial, and gas chromatography-tandem mass spectrometric detection. The HS-SPME parameters were optimized as follows: selection of CAR/PDMS fiber, 0.5% 2-(bromomethyl)naphthalene, 250 mg/L 15-crown-5-ether as a phase transfer catalyst, extraction and derivatization temperature of 95 °C, heating time of 20 min and pH of 7.0. Under the established conditions, the lowest limits of detection were 9 and 11 μg/L in 1.0 ml of plasma and urine, respectively, and the intra- and inter-day relative standard deviation was less than 7.7% at concentrations of 0.1 and 1.0 mg/L. The calibration curve showed good linearity of plasma and urine with r=0.9990 and r=0.9992, respectively. This method is simple, amenable to automation and environmentally friendly.

Authors+Show Affiliations

Department of Environmental Science, Kongju National University, Kongju, 314-701, South Korea.Department of Environmental Education, Kongju National University, Kongju 314-701, South Korea. Electronic address: hshin@kongju.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26162669

Citation

Kwon, Sun-Myung, and Ho-Sang Shin. "Simple Determination of Fluoride in Biological Samples By Headspace Solid-phase Microextraction and Gas Chromatography-tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1407, 2015, pp. 216-21.
Kwon SM, Shin HS. Simple determination of fluoride in biological samples by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry. J Chromatogr A. 2015;1407:216-21.
Kwon, S. M., & Shin, H. S. (2015). Simple determination of fluoride in biological samples by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry. Journal of Chromatography. A, 1407, 216-21. https://doi.org/10.1016/j.chroma.2015.06.066
Kwon SM, Shin HS. Simple Determination of Fluoride in Biological Samples By Headspace Solid-phase Microextraction and Gas Chromatography-tandem Mass Spectrometry. J Chromatogr A. 2015 Aug 14;1407:216-21. PubMed PMID: 26162669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simple determination of fluoride in biological samples by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry. AU - Kwon,Sun-Myung, AU - Shin,Ho-Sang, Y1 - 2015/07/02/ PY - 2015/04/07/received PY - 2015/06/27/revised PY - 2015/06/29/accepted PY - 2015/7/12/entrez PY - 2015/7/15/pubmed PY - 2015/10/7/medline KW - Biological samples KW - Fluoride KW - Gas chromatography–tandem mass spectrometry KW - Headspace solid phase microextraction SP - 216 EP - 21 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1407 N2 - A simple and convenient method to detect fluoride in biological samples was developed. This method was based on derivatization with 2-(bromomethyl)naphthalene, headspace solid phase microextraction (HS-SPME) in a vial, and gas chromatography-tandem mass spectrometric detection. The HS-SPME parameters were optimized as follows: selection of CAR/PDMS fiber, 0.5% 2-(bromomethyl)naphthalene, 250 mg/L 15-crown-5-ether as a phase transfer catalyst, extraction and derivatization temperature of 95 °C, heating time of 20 min and pH of 7.0. Under the established conditions, the lowest limits of detection were 9 and 11 μg/L in 1.0 ml of plasma and urine, respectively, and the intra- and inter-day relative standard deviation was less than 7.7% at concentrations of 0.1 and 1.0 mg/L. The calibration curve showed good linearity of plasma and urine with r=0.9990 and r=0.9992, respectively. This method is simple, amenable to automation and environmentally friendly. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/26162669/Simple_determination_of_fluoride_in_biological_samples_by_headspace_solid_phase_microextraction_and_gas_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(15)00930-9 DB - PRIME DP - Unbound Medicine ER -