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Development and Validation of an in vitro Experimental GastroIntestinal Dialysis Model with Colon Phase to Study the Availability and Colonic Metabolisation of Polyphenolic Compounds.
Planta Med. 2015 Aug; 81(12-13):1075-83.PM

Abstract

The biological effects of polyphenols depend on their mechanism of action in the body. This is affected by bioconversion by colon microbiota and absorption of colonic metabolites. We developed and validated an in vitro continuous flow dialysis model with colon phase (GastroIntestinal dialysis model with colon phase) to study the gastrointestinal metabolism and absorption of phenolic food constituents. Chlorogenic acid was used as model compound. The physiological conditions during gastrointestinal digestion were mimicked. A continuous flow dialysis system simulated the one-way absorption by passive diffusion from lumen to mucosa. The colon phase was developed using pooled faecal suspensions. Several methodological aspects including implementation of an anaerobic environment, adapted Wilkins Chalgren broth medium, 1.10(8) CFU/mL bacteria suspension as inoculum, pH adaptation to 5.8 and implementation of the dialysis system were conducted. Validation of the GastroIntestinal dialysis model with colon phase system showed a good recovery and precision (CV < 16 %). Availability of chlorogenic acid in the small intestinal phase (37 ± 3 %) of the GastroIntestinal dialysis model with colon phase is comparable with in vivo studies on ileostomy patients. In the colon phase, the human faecal microbiota deconjugated chlorogenic acid to caffeic acid, 3,4-dihydroxyphenyl propionic acid, 4-hydroxybenzoic acid, 3- or 4-hydroxyphenyl acetic acid, 2-methoxy-4-methylphenol and 3-phenylpropionic acid. The GastroIntestinal dialysis model with colon phase is a new, reliable gastrointestinal simulation system. It permits a fast and easy way to predict the availability of complex secondary metabolites, and to detect metabolites in an early stage after digestion. Isolation and identification of these metabolites may be used as references for in vivo bioavailability experiments and for investigating their bioactivity in in vitro experiments.

Authors+Show Affiliations

NatuRA - Laboratory of Nutrition and Functional Food Science, Department of Pharmaceutical Sciences, University of Antwerp, Belgium.NatuRA - Laboratory of Nutrition and Functional Food Science, Department of Pharmaceutical Sciences, University of Antwerp, Belgium.Laboratory of Bio-Organic Mass Spectrometry, Department of Pharmaceutical Sciences, University of Antwerp, Belgium.Laboratory of Bio-Organic Mass Spectrometry, Department of Pharmaceutical Sciences, University of Antwerp, Belgium.Laboratory of Microbiology, Parasitology and Hygiene, Department of Pharmaceutical Sciences, University of Antwerp, Belgium.NatuRA - Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences, University of Antwerp, Belgium.NatuRA - Laboratory of Nutrition and Functional Food Science, Department of Pharmaceutical Sciences, University of Antwerp, Belgium.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

26166134

Citation

Breynaert, Annelies, et al. "Development and Validation of an in Vitro Experimental GastroIntestinal Dialysis Model With Colon Phase to Study the Availability and Colonic Metabolisation of Polyphenolic Compounds." Planta Medica, vol. 81, no. 12-13, 2015, pp. 1075-83.
Breynaert A, Bosscher D, Kahnt A, et al. Development and Validation of an in vitro Experimental GastroIntestinal Dialysis Model with Colon Phase to Study the Availability and Colonic Metabolisation of Polyphenolic Compounds. Planta Med. 2015;81(12-13):1075-83.
Breynaert, A., Bosscher, D., Kahnt, A., Claeys, M., Cos, P., Pieters, L., & Hermans, N. (2015). Development and Validation of an in vitro Experimental GastroIntestinal Dialysis Model with Colon Phase to Study the Availability and Colonic Metabolisation of Polyphenolic Compounds. Planta Medica, 81(12-13), 1075-83. https://doi.org/10.1055/s-0035-1546154
Breynaert A, et al. Development and Validation of an in Vitro Experimental GastroIntestinal Dialysis Model With Colon Phase to Study the Availability and Colonic Metabolisation of Polyphenolic Compounds. Planta Med. 2015;81(12-13):1075-83. PubMed PMID: 26166134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and Validation of an in vitro Experimental GastroIntestinal Dialysis Model with Colon Phase to Study the Availability and Colonic Metabolisation of Polyphenolic Compounds. AU - Breynaert,Annelies, AU - Bosscher,Douwina, AU - Kahnt,Ariane, AU - Claeys,Magda, AU - Cos,Paul, AU - Pieters,Luc, AU - Hermans,Nina, Y1 - 2015/07/10/ PY - 2015/7/14/entrez PY - 2015/7/15/pubmed PY - 2016/7/7/medline SP - 1075 EP - 83 JF - Planta medica JO - Planta Med VL - 81 IS - 12-13 N2 - The biological effects of polyphenols depend on their mechanism of action in the body. This is affected by bioconversion by colon microbiota and absorption of colonic metabolites. We developed and validated an in vitro continuous flow dialysis model with colon phase (GastroIntestinal dialysis model with colon phase) to study the gastrointestinal metabolism and absorption of phenolic food constituents. Chlorogenic acid was used as model compound. The physiological conditions during gastrointestinal digestion were mimicked. A continuous flow dialysis system simulated the one-way absorption by passive diffusion from lumen to mucosa. The colon phase was developed using pooled faecal suspensions. Several methodological aspects including implementation of an anaerobic environment, adapted Wilkins Chalgren broth medium, 1.10(8) CFU/mL bacteria suspension as inoculum, pH adaptation to 5.8 and implementation of the dialysis system were conducted. Validation of the GastroIntestinal dialysis model with colon phase system showed a good recovery and precision (CV < 16 %). Availability of chlorogenic acid in the small intestinal phase (37 ± 3 %) of the GastroIntestinal dialysis model with colon phase is comparable with in vivo studies on ileostomy patients. In the colon phase, the human faecal microbiota deconjugated chlorogenic acid to caffeic acid, 3,4-dihydroxyphenyl propionic acid, 4-hydroxybenzoic acid, 3- or 4-hydroxyphenyl acetic acid, 2-methoxy-4-methylphenol and 3-phenylpropionic acid. The GastroIntestinal dialysis model with colon phase is a new, reliable gastrointestinal simulation system. It permits a fast and easy way to predict the availability of complex secondary metabolites, and to detect metabolites in an early stage after digestion. Isolation and identification of these metabolites may be used as references for in vivo bioavailability experiments and for investigating their bioactivity in in vitro experiments. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/26166134/Development_and_Validation_of_an_in_vitro_Experimental_GastroIntestinal_Dialysis_Model_with_Colon_Phase_to_Study_the_Availability_and_Colonic_Metabolisation_of_Polyphenolic_Compounds_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0035-1546154 DB - PRIME DP - Unbound Medicine ER -