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Knockdown of NF-κB p65 subunit expression suppresses growth of nude mouse lung tumour cell xenografts by inhibition of Bcl-2 apoptotic pathway.
Cell Biochem Funct. 2015 Jul; 33(5):320-5.CB

Abstract

Nuclear factor-kappaB (NF-κB) is an important transcriptional factor and regulates a variety of pathophysiologic process involved in cell survival and death. This study aimed to assess the effects of NF-κB p65 subunit knockdown in suppression of nude mouse lung tumour cell xenografts and understands the underlying molecular events. A nude mouse Lewis lung carcinoma cell xenograft model was established, and the mice were intraperitoneally injected with NF-κB p65 small interfering RNA (siRNA) and sacrificed after 2weeks of tumour cell injection. Tumour xenografts were harvested for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, Western blot and quantitative reverse transcription-polymerase chain reaction analyses. Results found that compared to the phosphate-buffered saline (PBS)-treated or the negative control (NC) siRNA-treated mice tumour xenograft weight and volume were significantly decreased in the NF-κB p65 siRNA-treated mice. The TUNEL positive (apoptosis) cells in xenograft sections were 45 ± 5 in PBS and 38 ± 3 in NC siRNA but increased to 271 ± 11 in p65 siRNA-treated mice. Compared to the PBS or the NC mice, levels of Bcl-2 mRNA and protein in tumour xenografts were significantly downregulated in p65 siRNA-treated mice. Knockdown of NF-κB p65 subunit expression can significantly inhibit the growth of nude mouse Lewis tumour cell xenografts by inducing tumour cell apoptosis and downregulating pro-apoptotic protein Bcl-2 expression. Targeting NF-κB p65 subunit expression as a potential therapeutic strategy needs further evaluation in treating human lung cancer.

Authors+Show Affiliations

Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, China.Department of Ophthalmology, The 4th Affiliated Hospital of China Medical University, Shenyang, China.Hospice Ward, Shengjing Hospital of China Medical University, Shenyang, China.Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, China.Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26178579

Citation

Qu, Yun, et al. "Knockdown of NF-κB P65 Subunit Expression Suppresses Growth of Nude Mouse Lung Tumour Cell Xenografts By Inhibition of Bcl-2 Apoptotic Pathway." Cell Biochemistry and Function, vol. 33, no. 5, 2015, pp. 320-5.
Qu Y, Qu B, Wang X, et al. Knockdown of NF-κB p65 subunit expression suppresses growth of nude mouse lung tumour cell xenografts by inhibition of Bcl-2 apoptotic pathway. Cell Biochem Funct. 2015;33(5):320-5.
Qu, Y., Qu, B., Wang, X., Wu, R., & Zhang, X. (2015). Knockdown of NF-κB p65 subunit expression suppresses growth of nude mouse lung tumour cell xenografts by inhibition of Bcl-2 apoptotic pathway. Cell Biochemistry and Function, 33(5), 320-5. https://doi.org/10.1002/cbf.3117
Qu Y, et al. Knockdown of NF-κB P65 Subunit Expression Suppresses Growth of Nude Mouse Lung Tumour Cell Xenografts By Inhibition of Bcl-2 Apoptotic Pathway. Cell Biochem Funct. 2015;33(5):320-5. PubMed PMID: 26178579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Knockdown of NF-κB p65 subunit expression suppresses growth of nude mouse lung tumour cell xenografts by inhibition of Bcl-2 apoptotic pathway. AU - Qu,Yun, AU - Qu,Bo, AU - Wang,Xiaofang, AU - Wu,Rong, AU - Zhang,Xiaoye, Y1 - 2015/07/15/ PY - 2014/10/24/received PY - 2015/04/20/revised PY - 2015/04/21/accepted PY - 2015/7/17/entrez PY - 2015/7/17/pubmed PY - 2016/2/6/medline KW - NF-κB KW - apoptosis KW - lung cancer KW - small interfering RNA KW - xenografts SP - 320 EP - 5 JF - Cell biochemistry and function JO - Cell Biochem Funct VL - 33 IS - 5 N2 - Nuclear factor-kappaB (NF-κB) is an important transcriptional factor and regulates a variety of pathophysiologic process involved in cell survival and death. This study aimed to assess the effects of NF-κB p65 subunit knockdown in suppression of nude mouse lung tumour cell xenografts and understands the underlying molecular events. A nude mouse Lewis lung carcinoma cell xenograft model was established, and the mice were intraperitoneally injected with NF-κB p65 small interfering RNA (siRNA) and sacrificed after 2weeks of tumour cell injection. Tumour xenografts were harvested for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, Western blot and quantitative reverse transcription-polymerase chain reaction analyses. Results found that compared to the phosphate-buffered saline (PBS)-treated or the negative control (NC) siRNA-treated mice tumour xenograft weight and volume were significantly decreased in the NF-κB p65 siRNA-treated mice. The TUNEL positive (apoptosis) cells in xenograft sections were 45 ± 5 in PBS and 38 ± 3 in NC siRNA but increased to 271 ± 11 in p65 siRNA-treated mice. Compared to the PBS or the NC mice, levels of Bcl-2 mRNA and protein in tumour xenografts were significantly downregulated in p65 siRNA-treated mice. Knockdown of NF-κB p65 subunit expression can significantly inhibit the growth of nude mouse Lewis tumour cell xenografts by inducing tumour cell apoptosis and downregulating pro-apoptotic protein Bcl-2 expression. Targeting NF-κB p65 subunit expression as a potential therapeutic strategy needs further evaluation in treating human lung cancer. SN - 1099-0844 UR - https://www.unboundmedicine.com/medline/citation/26178579/Knockdown_of_NF_κB_p65_subunit_expression_suppresses_growth_of_nude_mouse_lung_tumour_cell_xenografts_by_inhibition_of_Bcl_2_apoptotic_pathway_ L2 - https://doi.org/10.1002/cbf.3117 DB - PRIME DP - Unbound Medicine ER -