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Dynein, kinesin and morphological changes in optic nerve axons in a rat model with cerebrospinal fluid pressure reduction: the Beijing Intracranial and Intraocular Pressure (iCOP) study.
Acta Ophthalmol. 2016 May; 94(3):266-75.AO

Abstract

PURPOSE

To examine the influence of experimentally reduced cerebrospinal fluid pressure (CSFP) as compared to elevated intraocular pressure (IOP) on axonal morphology and axonal motor proteins in retinal ganglion cells (RGCs).

METHODS

The experimental study included 39 rats which underwent cerebrospinal fluid drainage for 6 hr, 30 rats which unilaterally underwent IOP elevation for 6 hr and 30 rats in a control group. Six hours after baseline, the animals were killed and the eyes were histologically and immunohistochemically examined.

RESULTS

In experimental models in the high-IOP group and the low-CSFP group as compared to the control group, RGC axons became abnormally dilated and accumulated vesicles. Both groups as compared to the control group showed an accumulation of dynein IC (intermediate chain) at the optic nerve head and retina and a reduction in kinesin HC (heavy chain) immunoreactivity in the optic nerve fibre axons. As a corollary, Western blot analysis revealed an elevation of dynein IC protein levels in the optic nerve head and retina and a decrease in kinesin HC protein levels in the optic nerve.

CONCLUSIONS

Experimental models with an acute IOP rise or with an acute CSFP reduction showed similar morphologic changes in the retinal ganglion cell axons and similar immunohistochemical changes in the axonal motor proteins kinesin HC and dynein IC. It supports the hypothesis that an experimental model with an acute reduction in CSFP as well as an experimental model with an acute rise in IOP may share similarities in the process of optic nerve damage.

Authors+Show Affiliations

Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China. Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing, China. Department of Ophthalmology, Medical Faculty Mannheim of the Ruprecht-Karls-University Heidelberg, Mannheim, Germany.Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China. Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China. Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26178710

Citation

Zhang, Zheng, et al. "Dynein, Kinesin and Morphological Changes in Optic Nerve Axons in a Rat Model With Cerebrospinal Fluid Pressure Reduction: the Beijing Intracranial and Intraocular Pressure (iCOP) Study." Acta Ophthalmologica, vol. 94, no. 3, 2016, pp. 266-75.
Zhang Z, Wu S, Jonas JB, et al. Dynein, kinesin and morphological changes in optic nerve axons in a rat model with cerebrospinal fluid pressure reduction: the Beijing Intracranial and Intraocular Pressure (iCOP) study. Acta Ophthalmol. 2016;94(3):266-75.
Zhang, Z., Wu, S., Jonas, J. B., Zhang, J., Liu, K., Lu, Q., & Wang, N. (2016). Dynein, kinesin and morphological changes in optic nerve axons in a rat model with cerebrospinal fluid pressure reduction: the Beijing Intracranial and Intraocular Pressure (iCOP) study. Acta Ophthalmologica, 94(3), 266-75. https://doi.org/10.1111/aos.12768
Zhang Z, et al. Dynein, Kinesin and Morphological Changes in Optic Nerve Axons in a Rat Model With Cerebrospinal Fluid Pressure Reduction: the Beijing Intracranial and Intraocular Pressure (iCOP) Study. Acta Ophthalmol. 2016;94(3):266-75. PubMed PMID: 26178710.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynein, kinesin and morphological changes in optic nerve axons in a rat model with cerebrospinal fluid pressure reduction: the Beijing Intracranial and Intraocular Pressure (iCOP) study. AU - Zhang,Zheng, AU - Wu,Shen, AU - Jonas,Jost B, AU - Zhang,Jingxue, AU - Liu,Kegao, AU - Lu,Qingjun, AU - Wang,Ningli, Y1 - 2015/07/14/ PY - 2015/03/21/revised PY - 2015/04/19/accepted PY - 2015/7/17/entrez PY - 2015/7/17/pubmed PY - 2016/12/15/medline KW - axonal transport KW - cerebrospinal fluid pressure KW - dynein KW - glaucoma KW - intraocular pressure KW - kinesin KW - optic neuropathy SP - 266 EP - 75 JF - Acta ophthalmologica JO - Acta Ophthalmol VL - 94 IS - 3 N2 - PURPOSE: To examine the influence of experimentally reduced cerebrospinal fluid pressure (CSFP) as compared to elevated intraocular pressure (IOP) on axonal morphology and axonal motor proteins in retinal ganglion cells (RGCs). METHODS: The experimental study included 39 rats which underwent cerebrospinal fluid drainage for 6 hr, 30 rats which unilaterally underwent IOP elevation for 6 hr and 30 rats in a control group. Six hours after baseline, the animals were killed and the eyes were histologically and immunohistochemically examined. RESULTS: In experimental models in the high-IOP group and the low-CSFP group as compared to the control group, RGC axons became abnormally dilated and accumulated vesicles. Both groups as compared to the control group showed an accumulation of dynein IC (intermediate chain) at the optic nerve head and retina and a reduction in kinesin HC (heavy chain) immunoreactivity in the optic nerve fibre axons. As a corollary, Western blot analysis revealed an elevation of dynein IC protein levels in the optic nerve head and retina and a decrease in kinesin HC protein levels in the optic nerve. CONCLUSIONS: Experimental models with an acute IOP rise or with an acute CSFP reduction showed similar morphologic changes in the retinal ganglion cell axons and similar immunohistochemical changes in the axonal motor proteins kinesin HC and dynein IC. It supports the hypothesis that an experimental model with an acute reduction in CSFP as well as an experimental model with an acute rise in IOP may share similarities in the process of optic nerve damage. SN - 1755-3768 UR - https://www.unboundmedicine.com/medline/citation/26178710/Dynein_kinesin_and_morphological_changes_in_optic_nerve_axons_in_a_rat_model_with_cerebrospinal_fluid_pressure_reduction:_the_Beijing_Intracranial_and_Intraocular_Pressure__iCOP__study_ L2 - https://doi.org/10.1111/aos.12768 DB - PRIME DP - Unbound Medicine ER -