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Rutin administration attenuates myocardial dysfunction in diabetic rats.
Cardiovasc Diabetol. 2015 Jul 17; 14:90.CD

Abstract

BACKGROUND

Oxidative stress plays a major role in diabetic cardiomyopathy pathogenesis. Anti-oxidant therapy has been investigated in preventing or treating several diabetic complications. However, anti-oxidant action on diabetic-induced cardiac remodeling is not completely clear. This study evaluated the effects of rutin, a flavonoid, on cardiac and myocardial function in diabetic rats.

METHODS

Wistar rats were assigned into control (C, n = 14); control-rutin (C-R, n = 14); diabetes mellitus (DM, n = 16); and DM-rutin (DM-R, n = 16) groups. Seven days after inducing diabetes (streptozotocin, 60 mg/kg, i.p.), rutin was injected intraperitoneally once a week (50 mg/kg) for 7 weeks. Echocardiogram was performed and myocardial function assessed in left ventricular (LV) papillary muscles. Serum insulin concentration was measured by ELISA.

STATISTICS

One-way ANOVA and Tukey's post hoc test.

RESULTS

Glycemia was higher in DM than DM-R and C and in DM-R than C-R. Insulin concentration was lower in diabetic groups than controls (C 2.45 ± 0.67; C-R 2.09 ± 0.52; DM 0.59 ± 0.18; DM-R 0.82 ± 0.21 ng/mL). Echocardiogram showed no differences between C-R and C. DM had increased LV systolic diameter compared to C, and increased left atrium diameter/body weight (BW) ratio and LV mass/BW ratio compared to C and DM-R. Septal wall thickness, LV diastolic diameter/BW ratio, and relative wall thickness were lower in DM-R than DM. Fractional shortening and posterior wall shortening velocity were lower in DM than C and DM-R. In papillary muscle preparation, DM and DM-R presented higher time to peak tension and time from peak tension to 50% relaxation than controls; time to peak tension was lower in DM-R than DM. Under 0.625 and 1.25 mM extracellular calcium concentrations, DM had higher developed tension than C.

CONCLUSION

Rutin attenuates cardiac remodeling and left ventricular and myocardial dysfunction caused by streptozotocin-induced diabetes mellitus.

Authors+Show Affiliations

Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil. drjullianoguimaraes@gmail.com.Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil. bmuzio@gmail.com.Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil. camilamrosa@hotmail.com.Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil. nascimentoaf@yahoo.com.br.Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil. mario.sugizaki@ig.com.br.Department of Chemistry and Biochemistry, Institute of Biosciences, Sao Paulo State University, UNESP, Botucatu, Brazil. angelica@ibb.unesp.br.Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil. cicogna@fmb.unesp.br.Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil. bioestatistica@ibb.unesp.br.Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil. mpoliti@fmb.unesp.br.Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil. katashi@fmb.unesp.br. Departamento de Clinica Medica, Faculdade de Medicina de Botucatu, UNESP Rubiao Junior, S/N 18618-970, Botucatu, SP, Brazil. katashi@fmb.unesp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26185015

Citation

Guimaraes, Julliano F C., et al. "Rutin Administration Attenuates Myocardial Dysfunction in Diabetic Rats." Cardiovascular Diabetology, vol. 14, 2015, p. 90.
Guimaraes JF, Muzio BP, Rosa CM, et al. Rutin administration attenuates myocardial dysfunction in diabetic rats. Cardiovasc Diabetol. 2015;14:90.
Guimaraes, J. F., Muzio, B. P., Rosa, C. M., Nascimento, A. F., Sugizaki, M. M., Fernandes, A. A., Cicogna, A. C., Padovani, C. R., Okoshi, M. P., & Okoshi, K. (2015). Rutin administration attenuates myocardial dysfunction in diabetic rats. Cardiovascular Diabetology, 14, 90. https://doi.org/10.1186/s12933-015-0255-7
Guimaraes JF, et al. Rutin Administration Attenuates Myocardial Dysfunction in Diabetic Rats. Cardiovasc Diabetol. 2015 Jul 17;14:90. PubMed PMID: 26185015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rutin administration attenuates myocardial dysfunction in diabetic rats. AU - Guimaraes,Julliano F C, AU - Muzio,Bruno P, AU - Rosa,Camila M, AU - Nascimento,Andre F, AU - Sugizaki,Mario M, AU - Fernandes,Ana A H, AU - Cicogna,Antonio C, AU - Padovani,Carlos R, AU - Okoshi,Marina P, AU - Okoshi,Katashi, Y1 - 2015/07/17/ PY - 2015/05/07/received PY - 2015/07/03/accepted PY - 2015/7/18/entrez PY - 2015/7/18/pubmed PY - 2016/4/22/medline SP - 90 EP - 90 JF - Cardiovascular diabetology JO - Cardiovasc Diabetol VL - 14 N2 - BACKGROUND: Oxidative stress plays a major role in diabetic cardiomyopathy pathogenesis. Anti-oxidant therapy has been investigated in preventing or treating several diabetic complications. However, anti-oxidant action on diabetic-induced cardiac remodeling is not completely clear. This study evaluated the effects of rutin, a flavonoid, on cardiac and myocardial function in diabetic rats. METHODS: Wistar rats were assigned into control (C, n = 14); control-rutin (C-R, n = 14); diabetes mellitus (DM, n = 16); and DM-rutin (DM-R, n = 16) groups. Seven days after inducing diabetes (streptozotocin, 60 mg/kg, i.p.), rutin was injected intraperitoneally once a week (50 mg/kg) for 7 weeks. Echocardiogram was performed and myocardial function assessed in left ventricular (LV) papillary muscles. Serum insulin concentration was measured by ELISA. STATISTICS: One-way ANOVA and Tukey's post hoc test. RESULTS: Glycemia was higher in DM than DM-R and C and in DM-R than C-R. Insulin concentration was lower in diabetic groups than controls (C 2.45 ± 0.67; C-R 2.09 ± 0.52; DM 0.59 ± 0.18; DM-R 0.82 ± 0.21 ng/mL). Echocardiogram showed no differences between C-R and C. DM had increased LV systolic diameter compared to C, and increased left atrium diameter/body weight (BW) ratio and LV mass/BW ratio compared to C and DM-R. Septal wall thickness, LV diastolic diameter/BW ratio, and relative wall thickness were lower in DM-R than DM. Fractional shortening and posterior wall shortening velocity were lower in DM than C and DM-R. In papillary muscle preparation, DM and DM-R presented higher time to peak tension and time from peak tension to 50% relaxation than controls; time to peak tension was lower in DM-R than DM. Under 0.625 and 1.25 mM extracellular calcium concentrations, DM had higher developed tension than C. CONCLUSION: Rutin attenuates cardiac remodeling and left ventricular and myocardial dysfunction caused by streptozotocin-induced diabetes mellitus. SN - 1475-2840 UR - https://www.unboundmedicine.com/medline/citation/26185015/Rutin_administration_attenuates_myocardial_dysfunction_in_diabetic_rats_ L2 - https://cardiab.biomedcentral.com/articles/10.1186/s12933-015-0255-7 DB - PRIME DP - Unbound Medicine ER -