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Rice Exo70 interacts with a fungal effector, AVR-Pii, and is required for AVR-Pii-triggered immunity.
Plant J. 2015 Sep; 83(5):875-87.PJ

Abstract

Vesicle trafficking including the exocytosis pathway is intimately associated with host immunity against pathogens. However, we still have insufficient knowledge about how it contributes to immunity, and how pathogen factors affect it. In this study, we explore host factors that interact with the Magnaporthe oryzae effector AVR-Pii. Gel filtration chromatography and co-immunoprecipitation assays identified a 150 kDa complex of proteins in the soluble fraction comprising AVR-Pii and OsExo70-F2 and OsExo70-F3, two rice Exo70 proteins presumably involved in exocytosis. Simultaneous knockdown of OsExo70-F2 and F3 totally abrogated Pii immune receptor-dependent resistance, but had no effect on Pia- and Pik-dependent resistance. Knockdown levels of OsExo70-F3 but not OsExo70-F2 correlated with reduction of Pii function, suggesting that OsExo70-F3 is specifically involved in Pii-dependent resistance. Under our current experimental conditions, over-expression of AVR-Pii or knockdown of OsExo70-F2 and -F3 genes in rice did not affect the virulence of compatible isolates of M. oryzae. AVR-Pii interaction with OsExo70-F3 appears to play a crucial role in immunity triggered by Pii, suggesting a role for OsExo70 as a decoy or helper in Pii/AVR-Pii interactions.

Authors+Show Affiliations

Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.Organization of Advanced Science and Technology, Kobe University, Kobe, Hyogo, Japan.Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26186703

Citation

Fujisaki, Koki, et al. "Rice Exo70 Interacts With a Fungal Effector, AVR-Pii, and Is Required for AVR-Pii-triggered Immunity." The Plant Journal : for Cell and Molecular Biology, vol. 83, no. 5, 2015, pp. 875-87.
Fujisaki K, Abe Y, Ito A, et al. Rice Exo70 interacts with a fungal effector, AVR-Pii, and is required for AVR-Pii-triggered immunity. Plant J. 2015;83(5):875-87.
Fujisaki, K., Abe, Y., Ito, A., Saitoh, H., Yoshida, K., Kanzaki, H., Kanzaki, E., Utsushi, H., Yamashita, T., Kamoun, S., & Terauchi, R. (2015). Rice Exo70 interacts with a fungal effector, AVR-Pii, and is required for AVR-Pii-triggered immunity. The Plant Journal : for Cell and Molecular Biology, 83(5), 875-87. https://doi.org/10.1111/tpj.12934
Fujisaki K, et al. Rice Exo70 Interacts With a Fungal Effector, AVR-Pii, and Is Required for AVR-Pii-triggered Immunity. Plant J. 2015;83(5):875-87. PubMed PMID: 26186703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rice Exo70 interacts with a fungal effector, AVR-Pii, and is required for AVR-Pii-triggered immunity. AU - Fujisaki,Koki, AU - Abe,Yoshiko, AU - Ito,Akiko, AU - Saitoh,Hiromasa, AU - Yoshida,Kentaro, AU - Kanzaki,Hiroyuki, AU - Kanzaki,Eiko, AU - Utsushi,Hiroe, AU - Yamashita,Tetsuro, AU - Kamoun,Sophien, AU - Terauchi,Ryohei, PY - 2015/05/06/received PY - 2015/06/26/revised PY - 2015/06/26/accepted PY - 2015/7/18/entrez PY - 2015/7/18/pubmed PY - 2016/6/9/medline KW - AVR-Pii KW - Exo70 KW - avirulence effector KW - decoy KW - effector-triggered immunity KW - helper SP - 875 EP - 87 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 83 IS - 5 N2 - Vesicle trafficking including the exocytosis pathway is intimately associated with host immunity against pathogens. However, we still have insufficient knowledge about how it contributes to immunity, and how pathogen factors affect it. In this study, we explore host factors that interact with the Magnaporthe oryzae effector AVR-Pii. Gel filtration chromatography and co-immunoprecipitation assays identified a 150 kDa complex of proteins in the soluble fraction comprising AVR-Pii and OsExo70-F2 and OsExo70-F3, two rice Exo70 proteins presumably involved in exocytosis. Simultaneous knockdown of OsExo70-F2 and F3 totally abrogated Pii immune receptor-dependent resistance, but had no effect on Pia- and Pik-dependent resistance. Knockdown levels of OsExo70-F3 but not OsExo70-F2 correlated with reduction of Pii function, suggesting that OsExo70-F3 is specifically involved in Pii-dependent resistance. Under our current experimental conditions, over-expression of AVR-Pii or knockdown of OsExo70-F2 and -F3 genes in rice did not affect the virulence of compatible isolates of M. oryzae. AVR-Pii interaction with OsExo70-F3 appears to play a crucial role in immunity triggered by Pii, suggesting a role for OsExo70 as a decoy or helper in Pii/AVR-Pii interactions. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/26186703/Rice_Exo70_interacts_with_a_fungal_effector_AVR_Pii_and_is_required_for_AVR_Pii_triggered_immunity_ L2 - https://doi.org/10.1111/tpj.12934 DB - PRIME DP - Unbound Medicine ER -