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Neuropeptide Y damages the integrity of mitochondrial structure and disrupts energy metabolism in cultured neonatal rat cardiomyocytes.
Peptides. 2015 Sep; 71:162-9.P

Abstract

Neuropeptide Y (NPY) plays an important role in cardiovascular diseases including stress cardiomyopathy, hypertrophic cardiomyopathy, heart failure, diabetic cardiomyopathy, hypertension, and so on. However, inconsistent results related to the role of NPY in the different types of cardiomyopathies make the exact involvement of the peptide elusive. Considering these effects are known to be involved in energy balance, as the hearts energy producer, the mitochondria, should be investigated, and not only mitochondrial structure but also its potential. Up to now, the impact of NPY on energy metabolism and mitochondria in cultured neonatal rat cardiomyocytes has not been reported. The main objective of our study was to test the role of NPY in cultured neonatal rat cardiomyocytes. After 24-h stimulation of NPY, the ATP content and activity of the cardiomyocytes were determined by Cell Counting Kit-8 and ATP-dependent bioluminescence assay kit, respectively. To further measure these effects, mitochondrial membrane potential was measured by JC-1 staining, the change of mitochondrial structure was detected by transmission electron microscopy, and the levels of PGC-1α (a marker of mitochondrial energy metabolism) mRNA and protein expression were determined by real-time PCR and Western blotting, respectively. The results showed that after 24-h stimulation of NPY, ATP content and activity in the cardiomyocytes were decreased. Moreover, cardiomyocyte mitochondria were changed in morphology. Further, a decline of mitochondrial membrane potential was induced in a dose-dependent manner and the levels of PGC-1α mRNA and protein expression were up-regulated after being treated by different dose of NPY. The results indicate that energy metabolism is suppressed, mitochondrial structure and membrane potential damaged, and PGC-α is changed in cultured neonatal rat cardiomyocytes after being treated by NPY.

Authors+Show Affiliations

Department of Forensic Medicine, Wuhan University School of Basic Medical Science, Wuhan, PR China; Department of Basic Medicine, Nanyang Medical College, Henan, PR China.Department of Forensic Medicine, Soochow University, Suzhou, PR China.Department of Forensic Medicine, Wuhan University School of Basic Medical Science, Wuhan, PR China.Department of Forensic Medicine, Soochow University, Suzhou, PR China.Department of Forensic Medicine, Wuhan University School of Basic Medical Science, Wuhan, PR China.Department of Forensic Medicine, Wuhan University School of Basic Medical Science, Wuhan, PR China. Electronic address: mengxiangzhi63@163.com.Department of Forensic Medicine, Soochow University, Suzhou, PR China.Department of Basic Medicine, Nanyang Medical College, Henan, PR China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26188175

Citation

Luo, Guochang, et al. "Neuropeptide Y Damages the Integrity of Mitochondrial Structure and Disrupts Energy Metabolism in Cultured Neonatal Rat Cardiomyocytes." Peptides, vol. 71, 2015, pp. 162-9.
Luo G, Xu X, Guo W, et al. Neuropeptide Y damages the integrity of mitochondrial structure and disrupts energy metabolism in cultured neonatal rat cardiomyocytes. Peptides. 2015;71:162-9.
Luo, G., Xu, X., Guo, W., Luo, C., Wang, H., Meng, X., Zhu, S., & Wei, Y. (2015). Neuropeptide Y damages the integrity of mitochondrial structure and disrupts energy metabolism in cultured neonatal rat cardiomyocytes. Peptides, 71, 162-9. https://doi.org/10.1016/j.peptides.2015.07.001
Luo G, et al. Neuropeptide Y Damages the Integrity of Mitochondrial Structure and Disrupts Energy Metabolism in Cultured Neonatal Rat Cardiomyocytes. Peptides. 2015;71:162-9. PubMed PMID: 26188175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuropeptide Y damages the integrity of mitochondrial structure and disrupts energy metabolism in cultured neonatal rat cardiomyocytes. AU - Luo,Guochang, AU - Xu,Xuehua, AU - Guo,Wei, AU - Luo,Chengliang, AU - Wang,Hao, AU - Meng,Xiangzhi, AU - Zhu,Shaohua, AU - Wei,Yan, Y1 - 2015/07/15/ PY - 2015/02/09/received PY - 2015/06/28/revised PY - 2015/07/03/accepted PY - 2015/7/19/entrez PY - 2015/7/19/pubmed PY - 2016/6/18/medline KW - Energy metabolism KW - Mitochondrial membrane potential KW - Mitochondrial structure KW - Neuropeptide Y KW - Proliferator-activated receptor γ coactivator-1alpha SP - 162 EP - 9 JF - Peptides JO - Peptides VL - 71 N2 - Neuropeptide Y (NPY) plays an important role in cardiovascular diseases including stress cardiomyopathy, hypertrophic cardiomyopathy, heart failure, diabetic cardiomyopathy, hypertension, and so on. However, inconsistent results related to the role of NPY in the different types of cardiomyopathies make the exact involvement of the peptide elusive. Considering these effects are known to be involved in energy balance, as the hearts energy producer, the mitochondria, should be investigated, and not only mitochondrial structure but also its potential. Up to now, the impact of NPY on energy metabolism and mitochondria in cultured neonatal rat cardiomyocytes has not been reported. The main objective of our study was to test the role of NPY in cultured neonatal rat cardiomyocytes. After 24-h stimulation of NPY, the ATP content and activity of the cardiomyocytes were determined by Cell Counting Kit-8 and ATP-dependent bioluminescence assay kit, respectively. To further measure these effects, mitochondrial membrane potential was measured by JC-1 staining, the change of mitochondrial structure was detected by transmission electron microscopy, and the levels of PGC-1α (a marker of mitochondrial energy metabolism) mRNA and protein expression were determined by real-time PCR and Western blotting, respectively. The results showed that after 24-h stimulation of NPY, ATP content and activity in the cardiomyocytes were decreased. Moreover, cardiomyocyte mitochondria were changed in morphology. Further, a decline of mitochondrial membrane potential was induced in a dose-dependent manner and the levels of PGC-1α mRNA and protein expression were up-regulated after being treated by different dose of NPY. The results indicate that energy metabolism is suppressed, mitochondrial structure and membrane potential damaged, and PGC-α is changed in cultured neonatal rat cardiomyocytes after being treated by NPY. SN - 1873-5169 UR - https://www.unboundmedicine.com/medline/citation/26188175/Neuropeptide_Y_damages_the_integrity_of_mitochondrial_structure_and_disrupts_energy_metabolism_in_cultured_neonatal_rat_cardiomyocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(15)00197-7 DB - PRIME DP - Unbound Medicine ER -