TY - JOUR T1 - A novel "dual-potential" electrochemiluminescence aptasensor array using CdS quantum dots and luminol-gold nanoparticles as labels for simultaneous detection of malachite green and chloramphenicol. AU - Feng,Xiaobin, AU - Gan,Ning, AU - Zhang,Huairong, AU - Yan,Qing, AU - Li,Tianhua, AU - Cao,Yuting, AU - Hu,Futao, AU - Yu,Hongwei, AU - Jiang,Qianli, Y1 - 2015/06/29/ PY - 2015/05/19/received PY - 2015/06/16/revised PY - 2015/06/19/accepted PY - 2015/7/21/entrez PY - 2015/7/21/pubmed PY - 2016/6/14/medline KW - Electrochemiluminescent aptasensor array KW - Malachite green and chloramphenicol KW - Quenching probes KW - Simultaneous detection SP - 587 EP - 93 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 74 N2 - A novel type of "dual-potential" electrochemiluminescence (ECL) aptasensor array was fabricated on a homemade screen-printed carbon electrode (SPCE) for simultaneous detection of malachite green (MG) and chloramphenicol (CAP) in one single assay. The SPCE substrate consisted of a common Ag/AgCl reference electrode, carbon counter electrode and two carbon working electrodes (WE1 and WE2). In the system, CdS quantum dots (QDs) were modified on WE1 as cathode ECL emitters and luminol-gold nanoparticles (L-Au NPs) were modified on WE2 as anode ECL emitters. Then the MG aptamer complementary strand (MG cDNA) and CAP aptamer complementary strand (CAP cDNA) were attached on CdS QDs and L-Au NPs, respectively. The cDNA would hybridize with corresponding aptamer that was respectively tagged with cyanine dye (Cy5) (as quenchers of CdS QDs) and chlorogenic acid (CA) (as quenchers of l-Au NPs) using poly(ethylenimine) (PEI) as a bridging agent. PEI could lead to a large number of quenchers on the aptamer, which increased the quenching efficiency. Upon MG and CAP adding, the targets could induce strand release due to the highly affinity of analytes toward aptamers. Meanwhile, it could release the Cy5 and CA, which recovered cathode ECL of CdS QDs and anode ECL of L-Au NPs simultaneously. This "dual-potential" ECL strategy could be used to detect MG and CAP with the linear ranges of 0.1-100 nM and 0.2-150 nM, with detection limits of 0.03 nM and 0.07 nM (at 3sB), respectively. More importantly, this designed method was successfully applied to determine MG and CAP in real fish samples and held great potential in the food analysis. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/26190470/A_novel_"dual_potential"_electrochemiluminescence_aptasensor_array_using_CdS_quantum_dots_and_luminol_gold_nanoparticles_as_labels_for_simultaneous_detection_of_malachite_green_and_chloramphenicol_ DB - PRIME DP - Unbound Medicine ER -