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An efficient signal-on aptamer-based biosensor for adenosine triphosphate detection using graphene oxide both as an electrochemical and electrochemiluminescence signal indicator.
Analyst. 2015 Sep 07; 140(17):6015-24.A

Abstract

An efficient aptasensor was developed in which graphene oxide (GO) was employed as an indicator for both electrochemical impedance spectroscopy and electrochemiluminescence (ECL) signal generation. The aptasensor was fabricated by self-assembling the ECL probe of a thiolated adenosine triphosphate binding aptamer (ABA) tagged with a Ru complex (Ru(bpy)3(2+) derivatives) onto the surface of gold nanoparticle (AuNP) modified glassy carbon electrode (GCE). ABA immobilized onto AuNP modified GCE could strongly adsorb GO due to the strong π-π interaction between ABA and graphene oxide; ECL quenching of the Ru complex then takes place because of energy transfer and electron transfer, and a large increase of the electron transfer resistance (Ret) of the electrode. While in the presence of target adenosine triphosphate (ATP), the ABA prefers to form ABA-ATP bioaffinity complexes, which have weak affinity to graphene oxide and keep the graphene oxide away from the electrode surface, thus allowing the ECL signal enhancement, and in conjunction with the decrease of the Ret. Because of the high ECL quenching efficiency, unique structure, and electronic properties of graphene oxide, the Ret and ECL intensity versus the logarithm of ATP concentration was linear in the wide range from 10 pM to 10 nM with an ultra-low detection limit of 6.7 pM to 4.8 pM, respectively. The proposed aptasensor exhibited excellent reproducibility, stability, and outstanding selectivity, and ATP could be effectively distinguished from its analogues. More significantly, this efficient ECL aptasensor strategy based on GO acting both as an electrochemical and ECL signal indicator is general and can be easily extended to other biological binding events.

Authors+Show Affiliations

Department of Chemistry and Key Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong 515063, P. R. China. whgao@stu.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26191542

Citation

Huang, Xiang, et al. "An Efficient Signal-on Aptamer-based Biosensor for Adenosine Triphosphate Detection Using Graphene Oxide Both as an Electrochemical and Electrochemiluminescence Signal Indicator." The Analyst, vol. 140, no. 17, 2015, pp. 6015-24.
Huang X, Li Y, Zhang X, et al. An efficient signal-on aptamer-based biosensor for adenosine triphosphate detection using graphene oxide both as an electrochemical and electrochemiluminescence signal indicator. Analyst. 2015;140(17):6015-24.
Huang, X., Li, Y., Zhang, X., Zhang, X., Chen, Y., & Gao, W. (2015). An efficient signal-on aptamer-based biosensor for adenosine triphosphate detection using graphene oxide both as an electrochemical and electrochemiluminescence signal indicator. The Analyst, 140(17), 6015-24. https://doi.org/10.1039/c5an00769k
Huang X, et al. An Efficient Signal-on Aptamer-based Biosensor for Adenosine Triphosphate Detection Using Graphene Oxide Both as an Electrochemical and Electrochemiluminescence Signal Indicator. Analyst. 2015 Sep 7;140(17):6015-24. PubMed PMID: 26191542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An efficient signal-on aptamer-based biosensor for adenosine triphosphate detection using graphene oxide both as an electrochemical and electrochemiluminescence signal indicator. AU - Huang,Xiang, AU - Li,Yuqin, AU - Zhang,Xiaoshan, AU - Zhang,Xin, AU - Chen,Yaowen, AU - Gao,Wenhua, PY - 2015/7/21/entrez PY - 2015/7/21/pubmed PY - 2016/2/20/medline SP - 6015 EP - 24 JF - The Analyst JO - Analyst VL - 140 IS - 17 N2 - An efficient aptasensor was developed in which graphene oxide (GO) was employed as an indicator for both electrochemical impedance spectroscopy and electrochemiluminescence (ECL) signal generation. The aptasensor was fabricated by self-assembling the ECL probe of a thiolated adenosine triphosphate binding aptamer (ABA) tagged with a Ru complex (Ru(bpy)3(2+) derivatives) onto the surface of gold nanoparticle (AuNP) modified glassy carbon electrode (GCE). ABA immobilized onto AuNP modified GCE could strongly adsorb GO due to the strong π-π interaction between ABA and graphene oxide; ECL quenching of the Ru complex then takes place because of energy transfer and electron transfer, and a large increase of the electron transfer resistance (Ret) of the electrode. While in the presence of target adenosine triphosphate (ATP), the ABA prefers to form ABA-ATP bioaffinity complexes, which have weak affinity to graphene oxide and keep the graphene oxide away from the electrode surface, thus allowing the ECL signal enhancement, and in conjunction with the decrease of the Ret. Because of the high ECL quenching efficiency, unique structure, and electronic properties of graphene oxide, the Ret and ECL intensity versus the logarithm of ATP concentration was linear in the wide range from 10 pM to 10 nM with an ultra-low detection limit of 6.7 pM to 4.8 pM, respectively. The proposed aptasensor exhibited excellent reproducibility, stability, and outstanding selectivity, and ATP could be effectively distinguished from its analogues. More significantly, this efficient ECL aptasensor strategy based on GO acting both as an electrochemical and ECL signal indicator is general and can be easily extended to other biological binding events. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/26191542/An_efficient_signal_on_aptamer_based_biosensor_for_adenosine_triphosphate_detection_using_graphene_oxide_both_as_an_electrochemical_and_electrochemiluminescence_signal_indicator_ DB - PRIME DP - Unbound Medicine ER -