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Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile.
Stem Cells Dev 2015; 24(23):2822-40SC

Abstract

Mesenchymal stromal cells (MSCs) are cultured cells that can give rise to mature mesenchymal cells under appropriate conditions and secrete a number of biologically relevant molecules that may play an important role in regenerative medicine. Evidence indicates that pericytes (PCs) correspond to mesenchymal stem cells in vivo and can give rise to MSCs when cultured, but a comparison between the gene expression profiles of cultured PCs (cPCs) and MSCs is lacking. We have devised a novel methodology to isolate PCs from human adipose tissue and compared cPCs to MSCs obtained through traditional methods. Freshly isolated PCs expressed CD34, CD140b, and CD271 on their surface, but not CD146. Both MSCs and cPCs were able to differentiate along mesenchymal pathways in vitro, displayed an essentially identical surface immunophenotype, and exhibited the ability to suppress CD3(+) lymphocyte proliferation in vitro. Microarray expression data of cPCs and MSCs formed a single cluster among other cell types. Further analyses showed that the gene expression profiles of cPCs and MSCs are extremely similar, although MSCs differentially expressed endothelial cell (EC)-specific transcripts. These results confirm, using the power of transcriptomic analysis, that PCs give rise to MSCs and suggest that low levels of ECs may persist in MSC cultures established using traditional protocols.

Authors+Show Affiliations

1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil . 2 Laboratory for Stem Cells and Tissue Engineering, PPGBioSaúde, Lutheran University of Brazil , Canoas, Brazil .1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .3 Laboratory of Large-Scale Functional Biology (LLSFBio), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .4 School of Pharmaceutical Sciences of Ribeirão Preto, Universidade de São Paulo , Ribeirão Preto, Brazil .1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .3 Laboratory of Large-Scale Functional Biology (LLSFBio), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil . 5 Department of Genetics, School of Medicine of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil . 6 Department of Clinical Medicine, School of Medicine of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26192741

Citation

da Silva Meirelles, Lindolfo, et al. "Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile." Stem Cells and Development, vol. 24, no. 23, 2015, pp. 2822-40.
da Silva Meirelles L, Malta TM, de Deus Wagatsuma VM, et al. Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile. Stem Cells Dev. 2015;24(23):2822-40.
da Silva Meirelles, L., Malta, T. M., de Deus Wagatsuma, V. M., Palma, P. V., Araújo, A. G., Ribeiro Malmegrim, K. C., ... Covas, D. T. (2015). Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile. Stem Cells and Development, 24(23), pp. 2822-40. doi:10.1089/scd.2015.0153.
da Silva Meirelles L, et al. Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile. Stem Cells Dev. 2015 Dec 1;24(23):2822-40. PubMed PMID: 26192741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile. AU - da Silva Meirelles,Lindolfo, AU - Malta,Tathiane Maistro, AU - de Deus Wagatsuma,Virgínia Mara, AU - Palma,Patrícia Viana Bonini, AU - Araújo,Amélia Goes, AU - Ribeiro Malmegrim,Kelen Cristina, AU - Morato de Oliveira,Fábio, AU - Panepucci,Rodrigo Alexandre, AU - Silva,Wilson Araújo,Jr AU - Kashima Haddad,Simone, AU - Covas,Dimas Tadeu, Y1 - 2015/08/19/ PY - 2015/7/21/entrez PY - 2015/7/21/pubmed PY - 2016/9/10/medline SP - 2822 EP - 40 JF - Stem cells and development JO - Stem Cells Dev. VL - 24 IS - 23 N2 - Mesenchymal stromal cells (MSCs) are cultured cells that can give rise to mature mesenchymal cells under appropriate conditions and secrete a number of biologically relevant molecules that may play an important role in regenerative medicine. Evidence indicates that pericytes (PCs) correspond to mesenchymal stem cells in vivo and can give rise to MSCs when cultured, but a comparison between the gene expression profiles of cultured PCs (cPCs) and MSCs is lacking. We have devised a novel methodology to isolate PCs from human adipose tissue and compared cPCs to MSCs obtained through traditional methods. Freshly isolated PCs expressed CD34, CD140b, and CD271 on their surface, but not CD146. Both MSCs and cPCs were able to differentiate along mesenchymal pathways in vitro, displayed an essentially identical surface immunophenotype, and exhibited the ability to suppress CD3(+) lymphocyte proliferation in vitro. Microarray expression data of cPCs and MSCs formed a single cluster among other cell types. Further analyses showed that the gene expression profiles of cPCs and MSCs are extremely similar, although MSCs differentially expressed endothelial cell (EC)-specific transcripts. These results confirm, using the power of transcriptomic analysis, that PCs give rise to MSCs and suggest that low levels of ECs may persist in MSC cultures established using traditional protocols. SN - 1557-8534 UR - https://www.unboundmedicine.com/medline/citation/26192741/Cultured_Human_Adipose_Tissue_Pericytes_and_Mesenchymal_Stromal_Cells_Display_a_Very_Similar_Gene_Expression_Profile_ L2 - https://www.liebertpub.com/doi/full/10.1089/scd.2015.0153?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -