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In vitro Xanthine Oxidase Inhibitory Studies of Lippia nodiflora and Isolated Flavonoids and Phenylethanoid Glycosides as Potential Uric Acid-lowering Agents.
Nat Prod Commun. 2015 Jun; 10(6):945-8.NP

Abstract

Lippia nodiflora has been traditionally used for treatment of knee joint pain. Hitherto, no studies have been reported on the effective use of L. nodiflora against hyperuricemia, gout or other metabolic disorders. In this present study, L. nodiflora was examined for its ability to lower uric acid levels using an in vitro xanthine oxidase inhibitory assay. The whole plant methanolic extract was subjected to bioactivity-guided fractionation to yield 4 fractions (F1-F4). F3 displayed the highest potency and was further purified by column chromatography to afford two phenylethanoid glycosides, arenarioside (1) and verbascoside (2), and three flavonoids, 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4), and nodifloretin (5). These compounds inhibited xanthine oxidase activity, with IC50 values between 7.52 ± 0.01 and 130.00 ± 2.25 μM, of which 3 was the most potent. In contrast, allopurinol, serving as a positive control, was 0.22 ± 0.00 μM. Thus, L. nodiflora, and its chemical constituents are worthy of further studies as potential anti-hyperuricemic agents.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26197523

Citation

Cheng, Lee-Chuen, et al. "In Vitro Xanthine Oxidase Inhibitory Studies of Lippia Nodiflora and Isolated Flavonoids and Phenylethanoid Glycosides as Potential Uric Acid-lowering Agents." Natural Product Communications, vol. 10, no. 6, 2015, pp. 945-8.
Cheng LC, Murugaiyah V, Chan KL. In vitro Xanthine Oxidase Inhibitory Studies of Lippia nodiflora and Isolated Flavonoids and Phenylethanoid Glycosides as Potential Uric Acid-lowering Agents. Nat Prod Commun. 2015;10(6):945-8.
Cheng, L. C., Murugaiyah, V., & Chan, K. L. (2015). In vitro Xanthine Oxidase Inhibitory Studies of Lippia nodiflora and Isolated Flavonoids and Phenylethanoid Glycosides as Potential Uric Acid-lowering Agents. Natural Product Communications, 10(6), 945-8.
Cheng LC, Murugaiyah V, Chan KL. In Vitro Xanthine Oxidase Inhibitory Studies of Lippia Nodiflora and Isolated Flavonoids and Phenylethanoid Glycosides as Potential Uric Acid-lowering Agents. Nat Prod Commun. 2015;10(6):945-8. PubMed PMID: 26197523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro Xanthine Oxidase Inhibitory Studies of Lippia nodiflora and Isolated Flavonoids and Phenylethanoid Glycosides as Potential Uric Acid-lowering Agents. AU - Cheng,Lee-Chuen, AU - Murugaiyah,Vikneswaran, AU - Chan,Kit-Lam, PY - 2015/7/23/entrez PY - 2015/7/23/pubmed PY - 2015/8/22/medline SP - 945 EP - 8 JF - Natural product communications JO - Nat Prod Commun VL - 10 IS - 6 N2 - Lippia nodiflora has been traditionally used for treatment of knee joint pain. Hitherto, no studies have been reported on the effective use of L. nodiflora against hyperuricemia, gout or other metabolic disorders. In this present study, L. nodiflora was examined for its ability to lower uric acid levels using an in vitro xanthine oxidase inhibitory assay. The whole plant methanolic extract was subjected to bioactivity-guided fractionation to yield 4 fractions (F1-F4). F3 displayed the highest potency and was further purified by column chromatography to afford two phenylethanoid glycosides, arenarioside (1) and verbascoside (2), and three flavonoids, 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4), and nodifloretin (5). These compounds inhibited xanthine oxidase activity, with IC50 values between 7.52 ± 0.01 and 130.00 ± 2.25 μM, of which 3 was the most potent. In contrast, allopurinol, serving as a positive control, was 0.22 ± 0.00 μM. Thus, L. nodiflora, and its chemical constituents are worthy of further studies as potential anti-hyperuricemic agents. SN - 1934-578X UR - https://www.unboundmedicine.com/medline/citation/26197523/In_vitro_Xanthine_Oxidase_Inhibitory_Studies_of_Lippia_nodiflora_and_Isolated_Flavonoids_and_Phenylethanoid_Glycosides_as_Potential_Uric_Acid_lowering_Agents_ DB - PRIME DP - Unbound Medicine ER -