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Metabolite Fingerprinting of Eugenia jambolana Fruit Pulp Extracts using NMR, HPLC-PDA-MS, GC-MS, MALDI-TOF-MS and ESI-MS/MS Spectrometry.
Nat Prod Commun. 2015 Jun; 10(6):969-76.NP

Abstract

Eugenia jambolana, commonly known as 'jamun' or Indian blackberry, is an important source of bioactive compounds. All parts of the plant like stem bark, leaves, flower, fruit pulp and seeds are traditionally used for many diseases. Metabolite profiling in medicinally important plants is critical to resolve the problems associated with standardization and quality control. Metabolite profiling of the fruit pulp of Jamun was performed by NMR, HPLC, MS, GC-MS and MALDI-TOF mass spectrometry. These hyphenated techniques helped in the identification of 68 chemically-diverse metabolites of the fruit pulp. These include anthocyanins, anthocyanidins, sugars, phenolics and volatile compounds. Five extracts of fruit pulp were prepared i.e. hexane, chloroform, ethylacetate, butanol and aqueous methanolic. Twenty-five metabolites identified and quantified in the n-butanol and aqueous-methanolic extracts of ripe jamun fruit by qNMR. LC-PDA-MS and MALDI-TOF spectrometry helped in deciphering thirty-nine metabolites out of which thirteen were quantified.

Authors

Sharma RJ
No affiliation info available
Gupta RC
No affiliation info available
Bansal AK
No affiliation info available
Singh IP
No affiliation info available

MeSH

Chromatography, High Pressure LiquidFruitGas Chromatography-Mass SpectrometryIndiaPlant ExtractsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSyzygiumTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26197529

Citation

Sharma, Ram Jee, et al. "Metabolite Fingerprinting of Eugenia Jambolana Fruit Pulp Extracts Using NMR, HPLC-PDA-MS, GC-MS, MALDI-TOF-MS and ESI-MS/MS Spectrometry." Natural Product Communications, vol. 10, no. 6, 2015, pp. 969-76.
Sharma RJ, Gupta RC, Bansal AK, et al. Metabolite Fingerprinting of Eugenia jambolana Fruit Pulp Extracts using NMR, HPLC-PDA-MS, GC-MS, MALDI-TOF-MS and ESI-MS/MS Spectrometry. Nat Prod Commun. 2015;10(6):969-76.
Sharma, R. J., Gupta, R. C., Bansal, A. K., & Singh, I. P. (2015). Metabolite Fingerprinting of Eugenia jambolana Fruit Pulp Extracts using NMR, HPLC-PDA-MS, GC-MS, MALDI-TOF-MS and ESI-MS/MS Spectrometry. Natural Product Communications, 10(6), 969-76.
Sharma RJ, et al. Metabolite Fingerprinting of Eugenia Jambolana Fruit Pulp Extracts Using NMR, HPLC-PDA-MS, GC-MS, MALDI-TOF-MS and ESI-MS/MS Spectrometry. Nat Prod Commun. 2015;10(6):969-76. PubMed PMID: 26197529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolite Fingerprinting of Eugenia jambolana Fruit Pulp Extracts using NMR, HPLC-PDA-MS, GC-MS, MALDI-TOF-MS and ESI-MS/MS Spectrometry. AU - Sharma,Ram Jee, AU - Gupta,Ramesh C, AU - Bansal,Arvind Kumar, AU - Singh,Inder Pal, PY - 2015/7/23/entrez PY - 2015/7/23/pubmed PY - 2015/8/22/medline SP - 969 EP - 76 JF - Natural product communications JO - Nat Prod Commun VL - 10 IS - 6 N2 - Eugenia jambolana, commonly known as 'jamun' or Indian blackberry, is an important source of bioactive compounds. All parts of the plant like stem bark, leaves, flower, fruit pulp and seeds are traditionally used for many diseases. Metabolite profiling in medicinally important plants is critical to resolve the problems associated with standardization and quality control. Metabolite profiling of the fruit pulp of Jamun was performed by NMR, HPLC, MS, GC-MS and MALDI-TOF mass spectrometry. These hyphenated techniques helped in the identification of 68 chemically-diverse metabolites of the fruit pulp. These include anthocyanins, anthocyanidins, sugars, phenolics and volatile compounds. Five extracts of fruit pulp were prepared i.e. hexane, chloroform, ethylacetate, butanol and aqueous methanolic. Twenty-five metabolites identified and quantified in the n-butanol and aqueous-methanolic extracts of ripe jamun fruit by qNMR. LC-PDA-MS and MALDI-TOF spectrometry helped in deciphering thirty-nine metabolites out of which thirteen were quantified. SN - 1934-578X UR - https://www.unboundmedicine.com/medline/citation/26197529/Metabolite_Fingerprinting_of_Eugenia_jambolana_Fruit_Pulp_Extracts_using_NMR_HPLC_PDA_MS_GC_MS_MALDI_TOF_MS_and_ESI_MS/MS_Spectrometry_ DB - PRIME DP - Unbound Medicine ER -