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Cytotoxicity and apoptosis induced by silver nanoparticles in human liver HepG2 cells in different dispersion media.
J Appl Toxicol. 2016 Mar; 36(3):352-60.JA

Abstract

Silver nanoparticles (Ag NPs) have been widely used in medical and healthcare products owing to their unique antibacterial activities. However, their safety for humans and the environment has not yet been established. This study evaluated the cellular proliferation and apoptosis of Ag NPs suspended in different solvents using human liver HepG2 cells. The ionization of Ag NPs in different dispersion media [deionized water, phosphate-buffered saline (PBS), saline and cell culture] was measured using an Ag ion selective electrode. The MTT assay was used to examine the cell proliferation activities. The effects of Ag NPs on cell cycle, induction of apoptosis, production of reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were analyzed using flow cytometry. The degree of Ag NPs ionization differed with dispersion media, with the concentrations of silver ions in deionized water being the highest in all suspensions. Ag NPs could inhibit the viability of HepG2 cells in a time- and concentration-dependent manner. Ag NPs (40, 80 and 160 µg ml(-1)) exposure could cause cell-cycle arrest in the G2/M phase, significantly increasing the apoptosis rate and ROS generation, and decreasing the MMP in HepG2 cells more sensitive to deionized water than in cell culture. These results suggested that the cellular toxicological mechanism of Ag NPs might be related to the oxidative stress of cells by the generation of ROS, leading to mitochondria injury and induction of apoptosis. It also implies that it is important to assess the physicochemical properties of NPs in the media where the biological toxicity tests are performed.

Authors+Show Affiliations

Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Jiangsu Key Laboratory for Biomaterials and Devices, School of Public Health, Southeast University, No.87 Dingjiaqiao, Nanjing, 210009, China.Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Jiangsu Key Laboratory for Biomaterials and Devices, School of Public Health, Southeast University, No.87 Dingjiaqiao, Nanjing, 210009, China.Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Jiangsu Key Laboratory for Biomaterials and Devices, School of Public Health, Southeast University, No.87 Dingjiaqiao, Nanjing, 210009, China.Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Jiangsu Key Laboratory for Biomaterials and Devices, School of Public Health, Southeast University, No.87 Dingjiaqiao, Nanjing, 210009, China.Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Jiangsu Key Laboratory for Biomaterials and Devices, School of Public Health, Southeast University, No.87 Dingjiaqiao, Nanjing, 210009, China.Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Jiangsu Key Laboratory for Biomaterials and Devices, School of Public Health, Southeast University, No.87 Dingjiaqiao, Nanjing, 210009, China.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26198703

Citation

Xue, Yuying, et al. "Cytotoxicity and Apoptosis Induced By Silver Nanoparticles in Human Liver HepG2 Cells in Different Dispersion Media." Journal of Applied Toxicology : JAT, vol. 36, no. 3, 2016, pp. 352-60.
Xue Y, Zhang T, Zhang B, et al. Cytotoxicity and apoptosis induced by silver nanoparticles in human liver HepG2 cells in different dispersion media. J Appl Toxicol. 2016;36(3):352-60.
Xue, Y., Zhang, T., Zhang, B., Gong, F., Huang, Y., & Tang, M. (2016). Cytotoxicity and apoptosis induced by silver nanoparticles in human liver HepG2 cells in different dispersion media. Journal of Applied Toxicology : JAT, 36(3), 352-60. https://doi.org/10.1002/jat.3199
Xue Y, et al. Cytotoxicity and Apoptosis Induced By Silver Nanoparticles in Human Liver HepG2 Cells in Different Dispersion Media. J Appl Toxicol. 2016;36(3):352-60. PubMed PMID: 26198703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxicity and apoptosis induced by silver nanoparticles in human liver HepG2 cells in different dispersion media. AU - Xue,Yuying, AU - Zhang,Ting, AU - Zhang,Bangyong, AU - Gong,Fan, AU - Huang,Yanmei, AU - Tang,Meng, Y1 - 2015/07/21/ PY - 2015/04/22/received PY - 2015/05/23/revised PY - 2015/05/24/accepted PY - 2015/7/23/entrez PY - 2015/7/23/pubmed PY - 2016/10/26/medline KW - HepG2 cells KW - apoptosis KW - cytotoxicity KW - dispersion media KW - silver nanoparticles SP - 352 EP - 60 JF - Journal of applied toxicology : JAT JO - J Appl Toxicol VL - 36 IS - 3 N2 - Silver nanoparticles (Ag NPs) have been widely used in medical and healthcare products owing to their unique antibacterial activities. However, their safety for humans and the environment has not yet been established. This study evaluated the cellular proliferation and apoptosis of Ag NPs suspended in different solvents using human liver HepG2 cells. The ionization of Ag NPs in different dispersion media [deionized water, phosphate-buffered saline (PBS), saline and cell culture] was measured using an Ag ion selective electrode. The MTT assay was used to examine the cell proliferation activities. The effects of Ag NPs on cell cycle, induction of apoptosis, production of reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were analyzed using flow cytometry. The degree of Ag NPs ionization differed with dispersion media, with the concentrations of silver ions in deionized water being the highest in all suspensions. Ag NPs could inhibit the viability of HepG2 cells in a time- and concentration-dependent manner. Ag NPs (40, 80 and 160 µg ml(-1)) exposure could cause cell-cycle arrest in the G2/M phase, significantly increasing the apoptosis rate and ROS generation, and decreasing the MMP in HepG2 cells more sensitive to deionized water than in cell culture. These results suggested that the cellular toxicological mechanism of Ag NPs might be related to the oxidative stress of cells by the generation of ROS, leading to mitochondria injury and induction of apoptosis. It also implies that it is important to assess the physicochemical properties of NPs in the media where the biological toxicity tests are performed. SN - 1099-1263 UR - https://www.unboundmedicine.com/medline/citation/26198703/Cytotoxicity_and_apoptosis_induced_by_silver_nanoparticles_in_human_liver_HepG2_cells_in_different_dispersion_media_ L2 - https://doi.org/10.1002/jat.3199 DB - PRIME DP - Unbound Medicine ER -