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Different routes, same pathways: Molecular mechanisms under silver ion and nanoparticle exposures in the soil sentinel Eisenia fetida.
Environ Pollut. 2015 Oct; 205:385-93.EP

Abstract

Use of nanotechnology products is increasing; with silver (Ag) nanoparticles particularly widely used. A key uncertainty surrounding the risk assessment of AgNPs is whether their effects are driven through the same mechanism of action that underlies the toxic effects of Ag ions. We present the first full transcriptome study of the effects of Ag ions and NPs in an ecotoxicological model soil invertebrate, the earthworm Eisenia fetida. Gene expression analyses indicated similar mechanisms for both silver forms with toxicity being exerted through pathways related to ribosome function, sugar and protein metabolism, molecular stress, disruption of energy production and histones. The main difference seen between Ag ions and NPs was associated with potential toxicokinetic effects related to cellular internalisation and communication, with pathways related to endocytosis and cilia being significantly enriched. These results point to a common final toxicodynamic response, but initial internalisation driven by different exposure routes and toxicokinetic mechanisms.

Authors+Show Affiliations

Organisms and Environment Division, Cardiff School of Biosciences, Cardiff University, Cardiff, CF10 3AT, UK. Electronic address: novom@cf.ac.uk.Centre for Ecology and Hydrology, Maclean Building, Wallingford, Oxfordshire, OX10 8BB, UK.Centre for Ecology and Hydrology, Maclean Building, Wallingford, Oxfordshire, OX10 8BB, UK.Centre for Ecology and Hydrology, Maclean Building, Wallingford, Oxfordshire, OX10 8BB, UK.Organisms and Environment Division, Cardiff School of Biosciences, Cardiff University, Cardiff, CF10 3AT, UK.Centre for Ecology and Hydrology, Maclean Building, Wallingford, Oxfordshire, OX10 8BB, UK.Centre for Ecology and Hydrology, Maclean Building, Wallingford, Oxfordshire, OX10 8BB, UK.Organisms and Environment Division, Cardiff School of Biosciences, Cardiff University, Cardiff, CF10 3AT, UK.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26204059

Citation

Novo, Marta, et al. "Different Routes, Same Pathways: Molecular Mechanisms Under Silver Ion and Nanoparticle Exposures in the Soil Sentinel Eisenia Fetida." Environmental Pollution (Barking, Essex : 1987), vol. 205, 2015, pp. 385-93.
Novo M, Lahive E, Díez-Ortiz M, et al. Different routes, same pathways: Molecular mechanisms under silver ion and nanoparticle exposures in the soil sentinel Eisenia fetida. Environ Pollut. 2015;205:385-93.
Novo, M., Lahive, E., Díez-Ortiz, M., Matzke, M., Morgan, A. J., Spurgeon, D. J., Svendsen, C., & Kille, P. (2015). Different routes, same pathways: Molecular mechanisms under silver ion and nanoparticle exposures in the soil sentinel Eisenia fetida. Environmental Pollution (Barking, Essex : 1987), 205, 385-93. https://doi.org/10.1016/j.envpol.2015.07.010
Novo M, et al. Different Routes, Same Pathways: Molecular Mechanisms Under Silver Ion and Nanoparticle Exposures in the Soil Sentinel Eisenia Fetida. Environ Pollut. 2015;205:385-93. PubMed PMID: 26204059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different routes, same pathways: Molecular mechanisms under silver ion and nanoparticle exposures in the soil sentinel Eisenia fetida. AU - Novo,Marta, AU - Lahive,Elma, AU - Díez-Ortiz,María, AU - Matzke,Marianne, AU - Morgan,Andrew J, AU - Spurgeon,David J, AU - Svendsen,Claus, AU - Kille,Peter, Y1 - 2015/07/20/ PY - 2015/04/18/received PY - 2015/07/03/revised PY - 2015/07/07/accepted PY - 2015/7/24/entrez PY - 2015/7/24/pubmed PY - 2016/3/29/medline KW - Cryptic lineage KW - Ions KW - Nanoparticles KW - Silver KW - Soil sentinel KW - Toxicokinetics SP - 385 EP - 93 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 205 N2 - Use of nanotechnology products is increasing; with silver (Ag) nanoparticles particularly widely used. A key uncertainty surrounding the risk assessment of AgNPs is whether their effects are driven through the same mechanism of action that underlies the toxic effects of Ag ions. We present the first full transcriptome study of the effects of Ag ions and NPs in an ecotoxicological model soil invertebrate, the earthworm Eisenia fetida. Gene expression analyses indicated similar mechanisms for both silver forms with toxicity being exerted through pathways related to ribosome function, sugar and protein metabolism, molecular stress, disruption of energy production and histones. The main difference seen between Ag ions and NPs was associated with potential toxicokinetic effects related to cellular internalisation and communication, with pathways related to endocytosis and cilia being significantly enriched. These results point to a common final toxicodynamic response, but initial internalisation driven by different exposure routes and toxicokinetic mechanisms. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/26204059/Different_routes_same_pathways:_Molecular_mechanisms_under_silver_ion_and_nanoparticle_exposures_in_the_soil_sentinel_Eisenia_fetida_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(15)00343-7 DB - PRIME DP - Unbound Medicine ER -