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Echovirus 30 meningitis epidemic followed by an outbreak-specific RT-qPCR.
J Clin Virol. 2015 Aug; 69:7-11.JC

Abstract

BACKGROUND

An outbreak of enteroviral aseptic meningitis emerged in Southwestern Finland in August 2009. The same enterovirus reappeared with increasing incidence of meningitis in other parts of Finland in 2010.

OBJECTIVES

To identify the incidence and molecular epidemiology of enteroviral meningitis outbreak.

STUDY DESIGN

The causative agent was identified as echovirus 30 (E-30) by sequencing partial viral protein 1 capsid genome, and a virus type-specific RT-qPCR was set up for sensitive detection of the virus in cerebrospinal fluid specimens. Enterovirus positive CSF specimens were subjected to the E-30-specific assay to investigate this unusual occurrence of aseptic meningitis and facilitate case confirmation during the outbreaks between August 2009 and September 2010.

RESULTS

E-30 was detected in 106 (72%) enterovirus positive cerebrospinal fluid specimens. All the meningitis cases in 2009 and most of them in 2010 were among adolescents and several were members of sport teams.

CONCLUSIONS

Between August 2009 and September 2010, E-30 caused an extensive outbreak with two peaks in Finland. Type-specific RT-PCR allowed rapid diagnostic follow-up of the epidemic.

Authors+Show Affiliations

Department of Virology, University of Turku, Finland. Electronic address: riikka.osterback@utu.fi.Department of Pediatrics, Turku University Hospital, Turku, Finland; Department of Pediatrics, Seinäjoki Central Hospital, Seinäjoki, Finland.Department of Pediatric Neurology, Turku University Hospital, Turku, Finland.Department of Pediatrics, Turku University Hospital, Turku, Finland.Department of Pediatrics, Turku University Hospital, Turku, Finland.Department of Virology, University of Turku, Finland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26209368

Citation

Österback, Riikka, et al. "Echovirus 30 Meningitis Epidemic Followed By an Outbreak-specific RT-qPCR." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 69, 2015, pp. 7-11.
Österback R, Kalliokoski T, Lähdesmäki T, et al. Echovirus 30 meningitis epidemic followed by an outbreak-specific RT-qPCR. J Clin Virol. 2015;69:7-11.
Österback, R., Kalliokoski, T., Lähdesmäki, T., Peltola, V., Ruuskanen, O., & Waris, M. (2015). Echovirus 30 meningitis epidemic followed by an outbreak-specific RT-qPCR. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 69, 7-11. https://doi.org/10.1016/j.jcv.2015.05.012
Österback R, et al. Echovirus 30 Meningitis Epidemic Followed By an Outbreak-specific RT-qPCR. J Clin Virol. 2015;69:7-11. PubMed PMID: 26209368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Echovirus 30 meningitis epidemic followed by an outbreak-specific RT-qPCR. AU - Österback,Riikka, AU - Kalliokoski,Teemu, AU - Lähdesmäki,Tuire, AU - Peltola,Ville, AU - Ruuskanen,Olli, AU - Waris,Matti, Y1 - 2015/05/22/ PY - 2014/10/28/received PY - 2015/04/22/revised PY - 2015/05/13/accepted PY - 2015/7/26/entrez PY - 2015/7/26/pubmed PY - 2016/6/15/medline KW - Echovirus 30 KW - Enterovirus KW - Meningitis KW - RT-qPCR SP - 7 EP - 11 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 69 N2 - BACKGROUND: An outbreak of enteroviral aseptic meningitis emerged in Southwestern Finland in August 2009. The same enterovirus reappeared with increasing incidence of meningitis in other parts of Finland in 2010. OBJECTIVES: To identify the incidence and molecular epidemiology of enteroviral meningitis outbreak. STUDY DESIGN: The causative agent was identified as echovirus 30 (E-30) by sequencing partial viral protein 1 capsid genome, and a virus type-specific RT-qPCR was set up for sensitive detection of the virus in cerebrospinal fluid specimens. Enterovirus positive CSF specimens were subjected to the E-30-specific assay to investigate this unusual occurrence of aseptic meningitis and facilitate case confirmation during the outbreaks between August 2009 and September 2010. RESULTS: E-30 was detected in 106 (72%) enterovirus positive cerebrospinal fluid specimens. All the meningitis cases in 2009 and most of them in 2010 were among adolescents and several were members of sport teams. CONCLUSIONS: Between August 2009 and September 2010, E-30 caused an extensive outbreak with two peaks in Finland. Type-specific RT-PCR allowed rapid diagnostic follow-up of the epidemic. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/26209368/Echovirus_30_meningitis_epidemic_followed_by_an_outbreak_specific_RT_qPCR_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(15)00157-2 DB - PRIME DP - Unbound Medicine ER -