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Cloning and characterization of microsomal triglyceride transfer protein gene and its potential connection with peroxisome proliferator-activated receptor (PPAR) in blunt snout bream (Megalobrama amblycephala).

Abstract

Microsomal triglyceride transfer protein (MTTP), a major intracellular protein capable of transferring neutral lipids, plays a pivotal role in the assembly and secretion of apolipoprotein B-containing lipoproteins. In this study, MTTP cDNA was firstly cloned from the liver of blunt snout bream (Megalobrama amblycephala), the full-length cDNA covered 3457-bp with an open reading frame of 2661-bp, which encodes 886 amino acids, including a putative signal peptide of 24 amino acids long. After the feeding trial, a graded tissue-specific expression pattern of MTTP was observed and high expression abundance in the liver and intestine indicated its major function in lipid transport in this fish species. In addition, expression of genes encoding MTTP as well as peroxisome proliferator-activated receptor (PPAR), which are transcription factors and serve as key regulators in lipid homoeostasis, was all affected by dietary lipid and choline supplementations. Elevated dietary lipid levels significantly increased the liver, intestinal and muscle MTTP mRNA abundance. Additionally, the down-regulation of MTTP expression in the liver and muscle was observed when fish were fed with inadequate choline supplementation in high-fat diet, yet up-regulated as supplementing extra choline in diet. Expressions of PPARα and PPARβ in the liver and muscle showed similar trend of MTTP expression. The results suggested the potential connection of MTTP and PPAR in response to different dietary nutritional factors. Furthermore, extra choline supplementations could promote lipid transfer and enhance fatty acid oxidation, which indicated a molecular mechanism of choline on diminishing fat accumulation in blunt snout bream.

Authors+Show Affiliations

Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China.Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China.Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China.Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China.Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China.Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China.Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China. Electronic address: wbliu@njau.edu.cn.Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China. Electronic address: xuweina@njau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26210738

Citation

Li, Jun-Yi, et al. "Cloning and Characterization of Microsomal Triglyceride Transfer Protein Gene and Its Potential Connection With Peroxisome Proliferator-activated Receptor (PPAR) in Blunt Snout Bream (Megalobrama Amblycephala)." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 189, 2015, pp. 23-33.
Li JY, Zhang DD, Jiang GZ, et al. Cloning and characterization of microsomal triglyceride transfer protein gene and its potential connection with peroxisome proliferator-activated receptor (PPAR) in blunt snout bream (Megalobrama amblycephala). Comp Biochem Physiol B, Biochem Mol Biol. 2015;189:23-33.
Li, J. Y., Zhang, D. D., Jiang, G. Z., Li, X. F., Zhang, C. N., Zhou, M., ... Xu, W. N. (2015). Cloning and characterization of microsomal triglyceride transfer protein gene and its potential connection with peroxisome proliferator-activated receptor (PPAR) in blunt snout bream (Megalobrama amblycephala). Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 189, pp. 23-33. doi:10.1016/j.cbpb.2015.07.004.
Li JY, et al. Cloning and Characterization of Microsomal Triglyceride Transfer Protein Gene and Its Potential Connection With Peroxisome Proliferator-activated Receptor (PPAR) in Blunt Snout Bream (Megalobrama Amblycephala). Comp Biochem Physiol B, Biochem Mol Biol. 2015;189:23-33. PubMed PMID: 26210738.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and characterization of microsomal triglyceride transfer protein gene and its potential connection with peroxisome proliferator-activated receptor (PPAR) in blunt snout bream (Megalobrama amblycephala). AU - Li,Jun-Yi, AU - Zhang,Ding-Dong, AU - Jiang,Guang-Zhen, AU - Li,Xiang-Fei, AU - Zhang,Chun-Nuan, AU - Zhou,Man, AU - Liu,Wen-Bin, AU - Xu,Wei-Na, Y1 - 2015/07/22/ PY - 2014/11/10/received PY - 2015/02/24/revised PY - 2015/07/14/accepted PY - 2015/7/27/entrez PY - 2015/7/27/pubmed PY - 2016/6/29/medline KW - Blunt snout bream KW - Choline supplementations KW - High-fat diet KW - Microsomal triglyceride transfer protein KW - Peroxisome proliferator-activated receptor SP - 23 EP - 33 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp. Biochem. Physiol. B, Biochem. Mol. Biol. VL - 189 N2 - Microsomal triglyceride transfer protein (MTTP), a major intracellular protein capable of transferring neutral lipids, plays a pivotal role in the assembly and secretion of apolipoprotein B-containing lipoproteins. In this study, MTTP cDNA was firstly cloned from the liver of blunt snout bream (Megalobrama amblycephala), the full-length cDNA covered 3457-bp with an open reading frame of 2661-bp, which encodes 886 amino acids, including a putative signal peptide of 24 amino acids long. After the feeding trial, a graded tissue-specific expression pattern of MTTP was observed and high expression abundance in the liver and intestine indicated its major function in lipid transport in this fish species. In addition, expression of genes encoding MTTP as well as peroxisome proliferator-activated receptor (PPAR), which are transcription factors and serve as key regulators in lipid homoeostasis, was all affected by dietary lipid and choline supplementations. Elevated dietary lipid levels significantly increased the liver, intestinal and muscle MTTP mRNA abundance. Additionally, the down-regulation of MTTP expression in the liver and muscle was observed when fish were fed with inadequate choline supplementation in high-fat diet, yet up-regulated as supplementing extra choline in diet. Expressions of PPARα and PPARβ in the liver and muscle showed similar trend of MTTP expression. The results suggested the potential connection of MTTP and PPAR in response to different dietary nutritional factors. Furthermore, extra choline supplementations could promote lipid transfer and enhance fatty acid oxidation, which indicated a molecular mechanism of choline on diminishing fat accumulation in blunt snout bream. SN - 1879-1107 UR - https://www.unboundmedicine.com/medline/citation/26210738/Cloning_and_characterization_of_microsomal_triglyceride_transfer_protein_gene_and_its_potential_connection_with_peroxisome_proliferator_activated_receptor__PPAR__in_blunt_snout_bream__Megalobrama_amblycephala__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-4959(15)00124-4 DB - PRIME DP - Unbound Medicine ER -