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Iron Deficiency and IL1β Polymorphisms in Helicobacter pylori-infected Children.
Helicobacter. 2016 Apr; 21(2):124-30.H

Abstract

BACKGROUND

Helicobacter pylori infection has been associated with an imbalance of iron homeostasis. IL-1β has been related with iron absorption disturbances through a variety of mechanisms. The aim of this study was to evaluate the presence of polymorphic variants for IL-1β cluster and gastric IL1β mRNA expression in H. pylori-infected children and their relationship with hypochlorhydria and iron deficiency (ID).

PATIENTS AND METHODS

Prospective study of 123 symptomatic children. At endoscopy, antral biopsies were taken for urease test, pathology and culture and blood for analysis of ferritin, transferrin, serum iron, and total iron-binding capacity. Polymorphisms in the IL-1β cluster (positions -511, -31, +3954, ILRN) were determined by PCR-RFLP. Gastric mucosal expression of IL-1β mRNA was determined by RT-PCR.

RESULTS

After exclusions, of 105 patients, 33 (31.4%) were H. pylori positive. Nine (8.6%) children were classified as iron deficient (ID). Helicobacter pylori positivity was associated with ID (OR: 5.1; 95% CI: 1.2-21.9) (p = .04). No significant differences were found in allele frequency for IL1β gene cluster polymorphisms between infected and uninfected children. Helicobacter pylori-infected children with ID had significantly increased gastric IL1β mRNA in comparison with infected children without ID. In addition, a significant positive correlation was observed between mucosal IL-1β mRNA and fasting gastric juice pH. Gastric pH values were significantly increased in H. pylori-infected patients with ID compared to uninfected children.

CONCLUSIONS

The established association between H. pylori infection and ID in children may be mediated by increased gastric mucosal IL-1β.

Authors+Show Affiliations

Department of Pediatric Gastroenterology and Nutrition, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.Department of Pediatric Gastroenterology and Nutrition, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.Laboratory of Molecular Microbiology, Celular and Molecular Biology Program, Faculty of Medicine, Universidad de Chile, Santiago, Chile.Leeds Institute of Biomedical and Clinical Sciences, University of Leeds, Leeds, UK.Department of Pediatric Gastroenterology and Nutrition, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26211930

Citation

Serrano, Carolina A., et al. "Iron Deficiency and IL1β Polymorphisms in Helicobacter Pylori-infected Children." Helicobacter, vol. 21, no. 2, 2016, pp. 124-30.
Serrano CA, Villagrán A, Toledo H, et al. Iron Deficiency and IL1β Polymorphisms in Helicobacter pylori-infected Children. Helicobacter. 2016;21(2):124-30.
Serrano, C. A., Villagrán, A., Toledo, H., Crabtree, J. E., & Harris, P. R. (2016). Iron Deficiency and IL1β Polymorphisms in Helicobacter pylori-infected Children. Helicobacter, 21(2), 124-30. https://doi.org/10.1111/hel.12247
Serrano CA, et al. Iron Deficiency and IL1β Polymorphisms in Helicobacter Pylori-infected Children. Helicobacter. 2016;21(2):124-30. PubMed PMID: 26211930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron Deficiency and IL1β Polymorphisms in Helicobacter pylori-infected Children. AU - Serrano,Carolina A, AU - Villagrán,Andrea, AU - Toledo,Héctor, AU - Crabtree,Jean E, AU - Harris,Paul R, Y1 - 2015/07/24/ PY - 2015/7/28/entrez PY - 2015/7/28/pubmed PY - 2016/12/15/medline KW - Helicobacter pylori KW - Interleukin-1β polymorphisms KW - children KW - iron deficiency SP - 124 EP - 30 JF - Helicobacter JO - Helicobacter VL - 21 IS - 2 N2 - BACKGROUND: Helicobacter pylori infection has been associated with an imbalance of iron homeostasis. IL-1β has been related with iron absorption disturbances through a variety of mechanisms. The aim of this study was to evaluate the presence of polymorphic variants for IL-1β cluster and gastric IL1β mRNA expression in H. pylori-infected children and their relationship with hypochlorhydria and iron deficiency (ID). PATIENTS AND METHODS: Prospective study of 123 symptomatic children. At endoscopy, antral biopsies were taken for urease test, pathology and culture and blood for analysis of ferritin, transferrin, serum iron, and total iron-binding capacity. Polymorphisms in the IL-1β cluster (positions -511, -31, +3954, ILRN) were determined by PCR-RFLP. Gastric mucosal expression of IL-1β mRNA was determined by RT-PCR. RESULTS: After exclusions, of 105 patients, 33 (31.4%) were H. pylori positive. Nine (8.6%) children were classified as iron deficient (ID). Helicobacter pylori positivity was associated with ID (OR: 5.1; 95% CI: 1.2-21.9) (p = .04). No significant differences were found in allele frequency for IL1β gene cluster polymorphisms between infected and uninfected children. Helicobacter pylori-infected children with ID had significantly increased gastric IL1β mRNA in comparison with infected children without ID. In addition, a significant positive correlation was observed between mucosal IL-1β mRNA and fasting gastric juice pH. Gastric pH values were significantly increased in H. pylori-infected patients with ID compared to uninfected children. CONCLUSIONS: The established association between H. pylori infection and ID in children may be mediated by increased gastric mucosal IL-1β. SN - 1523-5378 UR - https://www.unboundmedicine.com/medline/citation/26211930/Iron_Deficiency_and_IL1β_Polymorphisms_in_Helicobacter_pylori_infected_Children_ L2 - https://doi.org/10.1111/hel.12247 DB - PRIME DP - Unbound Medicine ER -