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miR-29c regulates NAV3 protein expression in a transgenic mouse model of Alzheimer's disease.
Brain Res. 2015 Oct 22; 1624:95-102.BR

Abstract

The microRNA-29 family (miRNA-29s) has three mature members, miR-29a, miR-29b and miR-29c, which have been implicated in the regulation of the pathogenesis of Alzheimer's disease (AD). The miR-29 family members exhibit differential regulation in various diseases and different subcellular distribution. In the present study, we initially investigated differential expression of miR-29c in the hippocampus and the frontal cortex of the young APPswe/PSΔE9 mouse brain, accompanied by inverse expression of neurone navigator 3 (NAV3), a regulator of axon guidance. We observed that miR-29c directly mediated downregulation of NAV3 protein expression in vitro. The mouse NAV3 mRNA has a functional miR-29c binding site in the 3' UTR, which localized in the position between 830-836 bp of 3'UTR region, slightly different from human NAV3 mRNA binding site. These observations suggest that miR-29c may be involved in neurodegenerative processes by regulating NAV3 expression in the young AD mouse.

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Authors+Show Affiliations

Zong Y
Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, PR China.
Yu P
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
Cheng H
Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, PR China.
Wang H
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
Wang X
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
Liang C
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
Zhu H
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
Qin Y
Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, PR China. Electronic address: qinyejun@163.com.
Qin C
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China. Electronic address: qinchuan@pumc.edu.cn.

MeSH

3' Untranslated RegionsAge FactorsAlzheimer DiseaseAmyloid beta-Protein PrecursorAnimalsBrainCell Line, TumorDisease Models, AnimalGene Expression RegulationGreen Fluorescent ProteinsHumansMembrane ProteinsMiceMice, TransgenicMicroRNAsMutationNerve Tissue ProteinsNeuroblastomaPresenilin-1Protein BindingRNA, Small Nuclear

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26212654

Citation

Zong, Yuanyuan, et al. "MiR-29c Regulates NAV3 Protein Expression in a Transgenic Mouse Model of Alzheimer's Disease." Brain Research, vol. 1624, 2015, pp. 95-102.
Zong Y, Yu P, Cheng H, et al. MiR-29c regulates NAV3 protein expression in a transgenic mouse model of Alzheimer's disease. Brain Res. 2015;1624:95-102.
Zong, Y., Yu, P., Cheng, H., Wang, H., Wang, X., Liang, C., Zhu, H., Qin, Y., & Qin, C. (2015). MiR-29c regulates NAV3 protein expression in a transgenic mouse model of Alzheimer's disease. Brain Research, 1624, 95-102. https://doi.org/10.1016/j.brainres.2015.07.022
Zong Y, et al. MiR-29c Regulates NAV3 Protein Expression in a Transgenic Mouse Model of Alzheimer's Disease. Brain Res. 2015 Oct 22;1624:95-102. PubMed PMID: 26212654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - miR-29c regulates NAV3 protein expression in a transgenic mouse model of Alzheimer's disease. AU - Zong,Yuanyuan, AU - Yu,Pin, AU - Cheng,Hongxia, AU - Wang,Hailin, AU - Wang,Xiaoying, AU - Liang,Chunlian, AU - Zhu,Hua, AU - Qin,Yejun, AU - Qin,Chuan, Y1 - 2015/07/23/ PY - 2014/09/09/received PY - 2015/07/15/revised PY - 2015/07/16/accepted PY - 2015/7/28/entrez PY - 2015/7/28/pubmed PY - 2016/8/5/medline KW - APPswe/PSΔE9 mice KW - Alzheimer׳s disease KW - NAV3 protein KW - miR-29c KW - microRNAs SP - 95 EP - 102 JF - Brain research JO - Brain Res VL - 1624 N2 - The microRNA-29 family (miRNA-29s) has three mature members, miR-29a, miR-29b and miR-29c, which have been implicated in the regulation of the pathogenesis of Alzheimer's disease (AD). The miR-29 family members exhibit differential regulation in various diseases and different subcellular distribution. In the present study, we initially investigated differential expression of miR-29c in the hippocampus and the frontal cortex of the young APPswe/PSΔE9 mouse brain, accompanied by inverse expression of neurone navigator 3 (NAV3), a regulator of axon guidance. We observed that miR-29c directly mediated downregulation of NAV3 protein expression in vitro. The mouse NAV3 mRNA has a functional miR-29c binding site in the 3' UTR, which localized in the position between 830-836 bp of 3'UTR region, slightly different from human NAV3 mRNA binding site. These observations suggest that miR-29c may be involved in neurodegenerative processes by regulating NAV3 expression in the young AD mouse. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/26212654/miR_29c_regulates_NAV3_protein_expression_in_a_transgenic_mouse_model_of_Alzheimer's_disease_ DB - PRIME DP - Unbound Medicine ER -