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Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum.
Genet Mol Res. 2015 Jul 14; 14(3):7811-20.GM

Abstract

Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 chromosomes; protein lengths ranged from 267-794 amino acids. Intron numbers ranged from 2-19 in the genes. The phylogenetic tree revealed that Hsp90 genes in tomato (Solanum lycopersicum L.), rice (Oryza sativa L.), and Arabidopsis (Arabidopsis thaliana L.) could be divided into 5 groups, which included 3 pairs of orthologous genes and 4 pairs of paralogous genes. Expression analysis of RNA-sequence data showed that the Hsp90-1 gene was specifically expressed in mature fruits, while Hsp90-5 and Hsp90-6 showed opposite expression patterns in various tissues of cultivated and wild tomatoes. The expression levels of the Hsp90-1, Hsp90-2, and Hsp90- 3 genes in various tissues of cultivated tomatoes were high, while both the expression levels of genes Hsp90-3 and Hsp90-4 were low. Additionally, quantitative real-time polymerase chain reaction showed that these genes were involved in the responses to yellow leaf curl virus in tomato plant leaves. Our results provide a foundation for identifying the function of the Hsp90 gene in tomato.

Authors+Show Affiliations

Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China zaiwenshan@sina.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26214462

Citation

Zai, W S., et al. "Comprehensive Identification and Expression Analysis of Hsp90s Gene Family in Solanum Lycopersicum." Genetics and Molecular Research : GMR, vol. 14, no. 3, 2015, pp. 7811-20.
Zai WS, Miao LX, Xiong ZL, et al. Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum. Genet Mol Res. 2015;14(3):7811-20.
Zai, W. S., Miao, L. X., Xiong, Z. L., Zhang, H. L., Ma, Y. R., Li, Y. L., Chen, Y. B., & Ye, S. G. (2015). Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum. Genetics and Molecular Research : GMR, 14(3), 7811-20. https://doi.org/10.4238/2015.July.14.7
Zai WS, et al. Comprehensive Identification and Expression Analysis of Hsp90s Gene Family in Solanum Lycopersicum. Genet Mol Res. 2015 Jul 14;14(3):7811-20. PubMed PMID: 26214462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum. AU - Zai,W S, AU - Miao,L X, AU - Xiong,Z L, AU - Zhang,H L, AU - Ma,Y R, AU - Li,Y L, AU - Chen,Y B, AU - Ye,S G, Y1 - 2015/07/14/ PY - 2015/7/28/entrez PY - 2015/7/28/pubmed PY - 2016/5/18/medline SP - 7811 EP - 20 JF - Genetics and molecular research : GMR JO - Genet Mol Res VL - 14 IS - 3 N2 - Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 chromosomes; protein lengths ranged from 267-794 amino acids. Intron numbers ranged from 2-19 in the genes. The phylogenetic tree revealed that Hsp90 genes in tomato (Solanum lycopersicum L.), rice (Oryza sativa L.), and Arabidopsis (Arabidopsis thaliana L.) could be divided into 5 groups, which included 3 pairs of orthologous genes and 4 pairs of paralogous genes. Expression analysis of RNA-sequence data showed that the Hsp90-1 gene was specifically expressed in mature fruits, while Hsp90-5 and Hsp90-6 showed opposite expression patterns in various tissues of cultivated and wild tomatoes. The expression levels of the Hsp90-1, Hsp90-2, and Hsp90- 3 genes in various tissues of cultivated tomatoes were high, while both the expression levels of genes Hsp90-3 and Hsp90-4 were low. Additionally, quantitative real-time polymerase chain reaction showed that these genes were involved in the responses to yellow leaf curl virus in tomato plant leaves. Our results provide a foundation for identifying the function of the Hsp90 gene in tomato. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/26214462/Comprehensive_identification_and_expression_analysis_of_Hsp90s_gene_family_in_Solanum_lycopersicum_ L2 - http://www.geneticsmr.com/articles/4715 DB - PRIME DP - Unbound Medicine ER -