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Model-based clustering of Escherichia coli O157:H7 genotypes and their potential association with clinical outcome in human infections.
Diagn Microbiol Infect Dis. 2015 Oct; 83(2):198-202.DM

Abstract

This study addresses the potential association of Escherichia coli O157:H7 genetic clusters with severe clinical manifestations in humans. The genotypes used in this model-based clustering had been delineated on the basis of lineage-specific polymorphism assay, Shiga toxin-encoding bacteriophage insertion site assay, clade typing, tir (A255T) polymorphism, variant analysis of Shiga toxin 2 gene, and antiterminator Q genes. Based on this model, the distribution of genotypes among tested strains suggested the presence of 6 main genetic clusters of E. coli O157:H7 strains. Clusters 1 and 3 were observed to be more frequent among E. coli O157:H7 strains isolated from bloody diarrhea and hemolytic uremic syndrome, respectively. Consequently, our findings supported the growing evidence of the existence of distinct genotypes of E. coli O157:H7 that differ in their virulence levels to human.

Authors+Show Affiliations

Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. Electronic address: mm_elhadidy@mans.edu.eg.Department of Microbiology & Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St. Abbassia, Cairo 11566, Egypt; Department of Pharmacy Practice, School of Pharmacy, Hampton University, Kittrell Hall Hampton, VA 23668, USA.Department of Microbiology, Belgian STEC/VTEC National Reference Centre for Human Microbiology, University Hospital Brussels (UZ Brussel), Laarbeeklaan, Belgium.Technology and Food Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Brusselsesteenweg 370, Melle 9090, Belgium.Technology and Food Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Brusselsesteenweg 370, Melle 9090, Belgium; Department of Pathology, Bacteriology and Poultry Diseases, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26219492

Citation

Elhadidy, Mohamed, et al. "Model-based Clustering of Escherichia Coli O157:H7 Genotypes and Their Potential Association With Clinical Outcome in Human Infections." Diagnostic Microbiology and Infectious Disease, vol. 83, no. 2, 2015, pp. 198-202.
Elhadidy M, Elkhatib WF, Piérard D, et al. Model-based clustering of Escherichia coli O157:H7 genotypes and their potential association with clinical outcome in human infections. Diagn Microbiol Infect Dis. 2015;83(2):198-202.
Elhadidy, M., Elkhatib, W. F., Piérard, D., De Reu, K., & Heyndrickx, M. (2015). Model-based clustering of Escherichia coli O157:H7 genotypes and their potential association with clinical outcome in human infections. Diagnostic Microbiology and Infectious Disease, 83(2), 198-202. https://doi.org/10.1016/j.diagmicrobio.2015.06.016
Elhadidy M, et al. Model-based Clustering of Escherichia Coli O157:H7 Genotypes and Their Potential Association With Clinical Outcome in Human Infections. Diagn Microbiol Infect Dis. 2015;83(2):198-202. PubMed PMID: 26219492.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Model-based clustering of Escherichia coli O157:H7 genotypes and their potential association with clinical outcome in human infections. AU - Elhadidy,Mohamed, AU - Elkhatib,Walid F, AU - Piérard,Denis, AU - De Reu,Koen, AU - Heyndrickx,Marc, Y1 - 2015/06/29/ PY - 2015/05/04/received PY - 2015/06/25/accepted PY - 2015/7/30/entrez PY - 2015/7/30/pubmed PY - 2016/6/9/medline KW - E. coli O157:H7 KW - Genetic clusters KW - Severe symptoms KW - Virulence SP - 198 EP - 202 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 83 IS - 2 N2 - This study addresses the potential association of Escherichia coli O157:H7 genetic clusters with severe clinical manifestations in humans. The genotypes used in this model-based clustering had been delineated on the basis of lineage-specific polymorphism assay, Shiga toxin-encoding bacteriophage insertion site assay, clade typing, tir (A255T) polymorphism, variant analysis of Shiga toxin 2 gene, and antiterminator Q genes. Based on this model, the distribution of genotypes among tested strains suggested the presence of 6 main genetic clusters of E. coli O157:H7 strains. Clusters 1 and 3 were observed to be more frequent among E. coli O157:H7 strains isolated from bloody diarrhea and hemolytic uremic syndrome, respectively. Consequently, our findings supported the growing evidence of the existence of distinct genotypes of E. coli O157:H7 that differ in their virulence levels to human. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/26219492/Model_based_clustering_of_Escherichia_coli_O157:H7_genotypes_and_their_potential_association_with_clinical_outcome_in_human_infections_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(15)00219-9 DB - PRIME DP - Unbound Medicine ER -