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Retino-protective effect of Bucida buceras against oxidative stress induced by H2O2 in human retinal pigment epithelial cells line.
BMC Complement Altern Med. 2015 Jul 29; 15:254.BC

Abstract

BACKGROUND

Reactive Oxygen Species (ROS) impair the physiological functions of Retinal Pigment Epithelial (RPE) cells, which are known as one major cause of age-related macular degeneration and retinopathy diseases. The purpose of this study is to explore the cytoprotective effects of the antioxidant Bucida buceras extract in co-treatment with hydrogen peroxide (H2O2) delivery as a single addition or with continuous generation using glucose oxidase (GOx) in ARPE-19 cell cultures. The mechanism of Bucida buceras extract is believed to be associated with their antioxidant capacity to protect cells against oxidative stress.

METHODS

A comparative oxidative stress H2O2-induced was performed by addition and enzymatic generation using glucose oxidase on human retinal pigment epithelial cells line. H2O2-induced injury was measured by toxic effects (cell death and apoptotic pathway) and intracellular redox status: glutathione (GSH), antioxidant enzymes (catalase and glutathione peroxidase) and reducing power (FRAP). The retino-protective effect of co-treatment with Bucida buceras extract on H2O2-induced human RPE cell injury was investigated by cell death (MTT assay) and oxidative stress biomarkers (H2O2, GSH, CAT, GPx and FRAP).

RESULTS

Bucida buceras L. extract is believed to be associated with the ability to prevent cellular oxidative stress. When added as a pulse, H2O2 is rapidly depleted and the cytotoxicity analyses show that cells can tolerate short exposure to high peroxide doses delivered as a pulse but are susceptible to lower chronic doses. Co-treatment with Bucida buceras was able to protect the cells against H2O2-induced injury. In addition to preventing cell death treatment with antioxidant plant could also reverse the significant decrease in GSH level, catalase activity and reducing power caused by H2O2.

CONCLUSION

These findings suggest that Bucida buceras could protect RPE against ocular pathogenesis associated with oxidative stress induced by H2O2-delivered by addition and enzymatic generation.

Links

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Authors+Show Affiliations

Iloki-Assanga SB
Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. ilokiasb@yahoo.com.
Lewis-Luján LM
Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. lidianyslewis@rubiopharma.com.
Fernández-Angulo D
Universidad de Sonora (UNISON) Hermosillo, Blvd. Luis Encinas y Rosales S/N Col. Centro, Hermosillo, Sonora, C.P. 83210, Mexico. dfernandez@rubiopharma.com.
Gil-Salido AA
Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. andreagil@libaf.com.
Lara-Espinoza CL
Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. claudialara@libaf.com.
Rubio-Pino JL
Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. medicinabiologica@rubiopharma.com.

MeSH

AntioxidantsCell LineCombretaceaeHumansHydrogen PeroxideOxidative StressOxidoreductasesPlant ExtractsRetinal Pigment Epithelium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26219933

Citation

Iloki-Assanga, Simon Bernard, et al. "Retino-protective Effect of Bucida Buceras Against Oxidative Stress Induced By H2O2 in Human Retinal Pigment Epithelial Cells Line." BMC Complementary and Alternative Medicine, vol. 15, 2015, p. 254.
Iloki-Assanga SB, Lewis-Luján LM, Fernández-Angulo D, et al. Retino-protective effect of Bucida buceras against oxidative stress induced by H2O2 in human retinal pigment epithelial cells line. BMC Complement Altern Med. 2015;15:254.
Iloki-Assanga, S. B., Lewis-Luján, L. M., Fernández-Angulo, D., Gil-Salido, A. A., Lara-Espinoza, C. L., & Rubio-Pino, J. L. (2015). Retino-protective effect of Bucida buceras against oxidative stress induced by H2O2 in human retinal pigment epithelial cells line. BMC Complementary and Alternative Medicine, 15, 254. https://doi.org/10.1186/s12906-015-0765-6
Iloki-Assanga SB, et al. Retino-protective Effect of Bucida Buceras Against Oxidative Stress Induced By H2O2 in Human Retinal Pigment Epithelial Cells Line. BMC Complement Altern Med. 2015 Jul 29;15:254. PubMed PMID: 26219933.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Retino-protective effect of Bucida buceras against oxidative stress induced by H2O2 in human retinal pigment epithelial cells line. AU - Iloki-Assanga,Simon Bernard, AU - Lewis-Luján,Lidianys María, AU - Fernández-Angulo,Daniela, AU - Gil-Salido,Armida Andrea, AU - Lara-Espinoza,Claudia Lizeth, AU - Rubio-Pino,José Luis, Y1 - 2015/07/29/ PY - 2014/12/18/received PY - 2015/07/06/accepted PY - 2015/7/30/entrez PY - 2015/7/30/pubmed PY - 2016/2/24/medline SP - 254 EP - 254 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 15 N2 - BACKGROUND: Reactive Oxygen Species (ROS) impair the physiological functions of Retinal Pigment Epithelial (RPE) cells, which are known as one major cause of age-related macular degeneration and retinopathy diseases. The purpose of this study is to explore the cytoprotective effects of the antioxidant Bucida buceras extract in co-treatment with hydrogen peroxide (H2O2) delivery as a single addition or with continuous generation using glucose oxidase (GOx) in ARPE-19 cell cultures. The mechanism of Bucida buceras extract is believed to be associated with their antioxidant capacity to protect cells against oxidative stress. METHODS: A comparative oxidative stress H2O2-induced was performed by addition and enzymatic generation using glucose oxidase on human retinal pigment epithelial cells line. H2O2-induced injury was measured by toxic effects (cell death and apoptotic pathway) and intracellular redox status: glutathione (GSH), antioxidant enzymes (catalase and glutathione peroxidase) and reducing power (FRAP). The retino-protective effect of co-treatment with Bucida buceras extract on H2O2-induced human RPE cell injury was investigated by cell death (MTT assay) and oxidative stress biomarkers (H2O2, GSH, CAT, GPx and FRAP). RESULTS: Bucida buceras L. extract is believed to be associated with the ability to prevent cellular oxidative stress. When added as a pulse, H2O2 is rapidly depleted and the cytotoxicity analyses show that cells can tolerate short exposure to high peroxide doses delivered as a pulse but are susceptible to lower chronic doses. Co-treatment with Bucida buceras was able to protect the cells against H2O2-induced injury. In addition to preventing cell death treatment with antioxidant plant could also reverse the significant decrease in GSH level, catalase activity and reducing power caused by H2O2. CONCLUSION: These findings suggest that Bucida buceras could protect RPE against ocular pathogenesis associated with oxidative stress induced by H2O2-delivered by addition and enzymatic generation. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/26219933/Retino_protective_effect_of_Bucida_buceras_against_oxidative_stress_induced_by_H2O2_in_human_retinal_pigment_epithelial_cells_line_ DB - PRIME DP - Unbound Medicine ER -