Tags

Type your tag names separated by a space and hit enter

Environmental Enrichment Ameliorates Neonatal Sevoflurane Exposure-Induced Cognitive and Synaptic Plasticity Impairments.
J Mol Neurosci. 2015 Nov; 57(3):358-65.JM

Abstract

Early exposure to sevoflurane, an inhalation anesthetic, induces neurodegeneration in the developing brain and subsequent long-term neurobehavioral abnormalities. Here, we investigated whether an enriched environment could mitigate neonatal sevoflurane exposure-induced long-term cognitive and synaptic plasticity impairments. Male C57BL/6 mice were exposed to 3 % sevoflurane 2 h daily for 3 days from postnatal day 6 (P6) to P8. The exposed mice were randomly allocated to an enriched environment for 2 h daily between P8 and P42 or to a standard environment. Their behavior and cognition were assessed using open field (P35) and fear conditioning tests (P41-P42). Hematoxylin-eosin staining was used to study morphological changes in pyramidal neurons of hippocampal CA1 and CA3 regions. Synaptic plasticity alternations were assessed using western blotting, Golgi staining, and electrophysiological recording. We found that sevoflurane-exposed mice housed in a standard environment exhibited a reduced freezing response in the contextual test, decreased number of dendritic spines on pyramidal neurons and synaptic plasticity-related proteins in the hippocampus, and impaired long-term potentiation. However, in an enriched environment, some of these abnormities induced by repeated sevoflurane exposure. In conclusion, neonatal sevoflurane exposure-induced cognitive and synaptic plasticity impairments are ameliorated by an enriched environment.

Authors+Show Affiliations

Department of Anesthesiology, Jinling Hospital, Nanjing Medical University, Nanjing, China.Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China.Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China.Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China.Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China.Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China.Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou, China.Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China.Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China.Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China. yjyangjj@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26227794

Citation

Ji, Mu-huo, et al. "Environmental Enrichment Ameliorates Neonatal Sevoflurane Exposure-Induced Cognitive and Synaptic Plasticity Impairments." Journal of Molecular Neuroscience : MN, vol. 57, no. 3, 2015, pp. 358-65.
Ji MH, Wang XM, Sun XR, et al. Environmental Enrichment Ameliorates Neonatal Sevoflurane Exposure-Induced Cognitive and Synaptic Plasticity Impairments. J Mol Neurosci. 2015;57(3):358-65.
Ji, M. H., Wang, X. M., Sun, X. R., Zhang, H., Ju, L. S., Qiu, L. L., Yang, J. J., Jia, M., Wu, J., & Yang, J. (2015). Environmental Enrichment Ameliorates Neonatal Sevoflurane Exposure-Induced Cognitive and Synaptic Plasticity Impairments. Journal of Molecular Neuroscience : MN, 57(3), 358-65. https://doi.org/10.1007/s12031-015-0627-1
Ji MH, et al. Environmental Enrichment Ameliorates Neonatal Sevoflurane Exposure-Induced Cognitive and Synaptic Plasticity Impairments. J Mol Neurosci. 2015;57(3):358-65. PubMed PMID: 26227794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Environmental Enrichment Ameliorates Neonatal Sevoflurane Exposure-Induced Cognitive and Synaptic Plasticity Impairments. AU - Ji,Mu-huo, AU - Wang,Xing-ming, AU - Sun,Xiao-ru, AU - Zhang,Hui, AU - Ju,Ling-sha, AU - Qiu,Li-li, AU - Yang,Jiao-jiao, AU - Jia,Min, AU - Wu,Jing, AU - Yang,Jianjun, Y1 - 2015/07/31/ PY - 2015/05/23/received PY - 2015/07/16/accepted PY - 2015/8/1/entrez PY - 2015/8/1/pubmed PY - 2016/8/11/medline KW - Long-term potentiation KW - Memory KW - Neonate KW - Sevoflurane KW - Synaptic plasticity SP - 358 EP - 65 JF - Journal of molecular neuroscience : MN JO - J. Mol. Neurosci. VL - 57 IS - 3 N2 - Early exposure to sevoflurane, an inhalation anesthetic, induces neurodegeneration in the developing brain and subsequent long-term neurobehavioral abnormalities. Here, we investigated whether an enriched environment could mitigate neonatal sevoflurane exposure-induced long-term cognitive and synaptic plasticity impairments. Male C57BL/6 mice were exposed to 3 % sevoflurane 2 h daily for 3 days from postnatal day 6 (P6) to P8. The exposed mice were randomly allocated to an enriched environment for 2 h daily between P8 and P42 or to a standard environment. Their behavior and cognition were assessed using open field (P35) and fear conditioning tests (P41-P42). Hematoxylin-eosin staining was used to study morphological changes in pyramidal neurons of hippocampal CA1 and CA3 regions. Synaptic plasticity alternations were assessed using western blotting, Golgi staining, and electrophysiological recording. We found that sevoflurane-exposed mice housed in a standard environment exhibited a reduced freezing response in the contextual test, decreased number of dendritic spines on pyramidal neurons and synaptic plasticity-related proteins in the hippocampus, and impaired long-term potentiation. However, in an enriched environment, some of these abnormities induced by repeated sevoflurane exposure. In conclusion, neonatal sevoflurane exposure-induced cognitive and synaptic plasticity impairments are ameliorated by an enriched environment. SN - 1559-1166 UR - https://www.unboundmedicine.com/medline/citation/26227794/Environmental_Enrichment_Ameliorates_Neonatal_Sevoflurane_Exposure_Induced_Cognitive_and_Synaptic_Plasticity_Impairments_ L2 - https://dx.doi.org/10.1007/s12031-015-0627-1 DB - PRIME DP - Unbound Medicine ER -