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Mangiferin Inhibits Renal Urate Reabsorption by Modulating Urate Transporters in Experimental Hyperuricemia.
Biol Pharm Bull. 2015; 38(10):1591-8.BP

Abstract

Mangiferin, a natural glucosyl xanthone from the leaves of Mangifera indica L., was previously shown to exert potent hypouricemic effects associated with inhibition of the activity of xanthine dehydrogenase/oxidase. The present study aimed to evaluate its uricosuric effect and possible molecular mechanisms underlying the renal urate transporters responsible for urate reabsorption in vivo. Mangiferin (1.5-24.0 mg/kg) was administered intragastrically to hyperuricemic mice and rats induced by the intraperitoneal injection of uric acid and potassium oxonate, respectively. The uricosuric effect was evaluated by determining the serum and urinary urate levels as well as fractional excretion of uric acid (FEUA). The mRNA and protein levels of renal urate-anion transporter 1 (URAT1), organic anion transporter 10 (OAT10), glucose transporter 9 (GLUT9), and PDZ domain-containing protein (PDZK1) were analyzed. The administration of mangiferin significantly decreased the serum urate levels in hyperuricemic mice in a dose- and time-dependent manner. In hyperuricemic rats, mangiferin also reduced the serum urate levels and increased the urinary urate levels and FEUA. These results indicate that mangiferin has uricosuric effects. Further examination showed that mangiferin markedly inhibited the mRNA and protein expression of renal URAT1, OAT10, and GLUT9 in hyperuricemic rats, but did not interfere with PDZK1 expression. Taken together, these findings suggest that mangiferin promotes urate excretion by the kidney, which may be related to the inhibition of urate reabsorption via downregulation of renal urate transporters.

Authors+Show Affiliations

Biomedical Engineering Research Center, Kunming Medical University.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26228630

Citation

Yang, Hua, et al. "Mangiferin Inhibits Renal Urate Reabsorption By Modulating Urate Transporters in Experimental Hyperuricemia." Biological & Pharmaceutical Bulletin, vol. 38, no. 10, 2015, pp. 1591-8.
Yang H, Gao L, Niu Y, et al. Mangiferin Inhibits Renal Urate Reabsorption by Modulating Urate Transporters in Experimental Hyperuricemia. Biol Pharm Bull. 2015;38(10):1591-8.
Yang, H., Gao, L., Niu, Y., Zhou, Y., Lin, H., Jiang, J., Kong, X., Liu, X., & Li, L. (2015). Mangiferin Inhibits Renal Urate Reabsorption by Modulating Urate Transporters in Experimental Hyperuricemia. Biological & Pharmaceutical Bulletin, 38(10), 1591-8. https://doi.org/10.1248/bpb.b15-00402
Yang H, et al. Mangiferin Inhibits Renal Urate Reabsorption By Modulating Urate Transporters in Experimental Hyperuricemia. Biol Pharm Bull. 2015;38(10):1591-8. PubMed PMID: 26228630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mangiferin Inhibits Renal Urate Reabsorption by Modulating Urate Transporters in Experimental Hyperuricemia. AU - Yang,Hua, AU - Gao,Lihui, AU - Niu,Yanfen, AU - Zhou,Yuanfang, AU - Lin,Hua, AU - Jiang,Jing, AU - Kong,Xiangfu, AU - Liu,Xu, AU - Li,Ling, Y1 - 2015/07/29/ PY - 2015/8/1/entrez PY - 2015/8/1/pubmed PY - 2016/7/28/medline SP - 1591 EP - 8 JF - Biological & pharmaceutical bulletin JO - Biol Pharm Bull VL - 38 IS - 10 N2 - Mangiferin, a natural glucosyl xanthone from the leaves of Mangifera indica L., was previously shown to exert potent hypouricemic effects associated with inhibition of the activity of xanthine dehydrogenase/oxidase. The present study aimed to evaluate its uricosuric effect and possible molecular mechanisms underlying the renal urate transporters responsible for urate reabsorption in vivo. Mangiferin (1.5-24.0 mg/kg) was administered intragastrically to hyperuricemic mice and rats induced by the intraperitoneal injection of uric acid and potassium oxonate, respectively. The uricosuric effect was evaluated by determining the serum and urinary urate levels as well as fractional excretion of uric acid (FEUA). The mRNA and protein levels of renal urate-anion transporter 1 (URAT1), organic anion transporter 10 (OAT10), glucose transporter 9 (GLUT9), and PDZ domain-containing protein (PDZK1) were analyzed. The administration of mangiferin significantly decreased the serum urate levels in hyperuricemic mice in a dose- and time-dependent manner. In hyperuricemic rats, mangiferin also reduced the serum urate levels and increased the urinary urate levels and FEUA. These results indicate that mangiferin has uricosuric effects. Further examination showed that mangiferin markedly inhibited the mRNA and protein expression of renal URAT1, OAT10, and GLUT9 in hyperuricemic rats, but did not interfere with PDZK1 expression. Taken together, these findings suggest that mangiferin promotes urate excretion by the kidney, which may be related to the inhibition of urate reabsorption via downregulation of renal urate transporters. SN - 1347-5215 UR - https://www.unboundmedicine.com/medline/citation/26228630/Mangiferin_Inhibits_Renal_Urate_Reabsorption_by_Modulating_Urate_Transporters_in_Experimental_Hyperuricemia_ L2 - https://dx.doi.org/10.1248/bpb.b15-00402 DB - PRIME DP - Unbound Medicine ER -