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Buccal Cytome Biomarkers and Their Association with Plasma Folate, Vitamin B12 and Homocysteine in Alzheimer's Disease.
J Nutrigenet Nutrigenomics 2015; 8(2):57-69JN

Abstract

BACKGROUND/AIMS

Alzheimer's disease (AD) is an irreversible neurodegenerative disorder and is the commonest form of dementia. One aim of this study was to determine whether AD individuals have altered plasma folate, vitamin B12 and homocysteine (Hcy) levels compared to controls. The other aim was to investigate correlations between B vitamins and buccal biomarkers to test whether they are influenced by B vitamin status.

METHODS

Folate, vitamin B12 and Hcy were measured using ARCHITECT® and AxSYM® assays. Genomic stability was measured using the buccal micronucleus cytome assay.

RESULTS

The area under the receiver operating characteristic curve for AD basal cells was 0.96 (p < 0.0001), for karyorrhectic cells 0.88 (p < 0.0001) and for basal and karyorrhectic cells 0.91 (p < 0.0001). Hcy was significantly increased (p = 0.0003) compared to controls. Plasma vitamin B12 in controls showed a positive correlation with pyknosis (r = 0.5365, p = 0.004), karyolysis (r = 0.5447, p = 0.004) and condensed chromatin (r = 0.5238, p = 0.006). Plasma vitamin B12 in AD cases showed a positive correlation with micronuclei (r = 0.3552, p = 0.04) and basal cells (r = 0.3448, p = 0.04), whilst plasma Hcy showed a negative correlation with karyorrhectic cells (r = -0.4107, p = 0.01).

CONCLUSIONS

Hcy was significantly increased in AD cases relative to controls. The lower frequency of basal cells and karyorrhectic cells observed in AD cases may be explained by lower vitamin B12 and higher Hcy levels, respectively.

Authors+Show Affiliations

Commonwealth and Scientific Industrial Research Organisation, Genome Health and Personalised Nutrition Laboratory, Food and Nutrition Flagship, Adelaide, S.A., Australia.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26228832

Citation

Thomas, Philip, and Michael Fenech. "Buccal Cytome Biomarkers and Their Association With Plasma Folate, Vitamin B12 and Homocysteine in Alzheimer's Disease." Journal of Nutrigenetics and Nutrigenomics, vol. 8, no. 2, 2015, pp. 57-69.
Thomas P, Fenech M. Buccal Cytome Biomarkers and Their Association with Plasma Folate, Vitamin B12 and Homocysteine in Alzheimer's Disease. J Nutrigenet Nutrigenomics. 2015;8(2):57-69.
Thomas, P., & Fenech, M. (2015). Buccal Cytome Biomarkers and Their Association with Plasma Folate, Vitamin B12 and Homocysteine in Alzheimer's Disease. Journal of Nutrigenetics and Nutrigenomics, 8(2), pp. 57-69. doi:10.1159/000435784.
Thomas P, Fenech M. Buccal Cytome Biomarkers and Their Association With Plasma Folate, Vitamin B12 and Homocysteine in Alzheimer's Disease. J Nutrigenet Nutrigenomics. 2015;8(2):57-69. PubMed PMID: 26228832.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Buccal Cytome Biomarkers and Their Association with Plasma Folate, Vitamin B12 and Homocysteine in Alzheimer's Disease. AU - Thomas,Philip, AU - Fenech,Michael, Y1 - 2015/07/28/ PY - 2014/12/16/received PY - 2015/06/04/accepted PY - 2015/8/1/entrez PY - 2015/8/1/pubmed PY - 2016/6/14/medline SP - 57 EP - 69 JF - Journal of nutrigenetics and nutrigenomics JO - J Nutrigenet Nutrigenomics VL - 8 IS - 2 N2 - BACKGROUND/AIMS: Alzheimer's disease (AD) is an irreversible neurodegenerative disorder and is the commonest form of dementia. One aim of this study was to determine whether AD individuals have altered plasma folate, vitamin B12 and homocysteine (Hcy) levels compared to controls. The other aim was to investigate correlations between B vitamins and buccal biomarkers to test whether they are influenced by B vitamin status. METHODS: Folate, vitamin B12 and Hcy were measured using ARCHITECT® and AxSYM® assays. Genomic stability was measured using the buccal micronucleus cytome assay. RESULTS: The area under the receiver operating characteristic curve for AD basal cells was 0.96 (p < 0.0001), for karyorrhectic cells 0.88 (p < 0.0001) and for basal and karyorrhectic cells 0.91 (p < 0.0001). Hcy was significantly increased (p = 0.0003) compared to controls. Plasma vitamin B12 in controls showed a positive correlation with pyknosis (r = 0.5365, p = 0.004), karyolysis (r = 0.5447, p = 0.004) and condensed chromatin (r = 0.5238, p = 0.006). Plasma vitamin B12 in AD cases showed a positive correlation with micronuclei (r = 0.3552, p = 0.04) and basal cells (r = 0.3448, p = 0.04), whilst plasma Hcy showed a negative correlation with karyorrhectic cells (r = -0.4107, p = 0.01). CONCLUSIONS: Hcy was significantly increased in AD cases relative to controls. The lower frequency of basal cells and karyorrhectic cells observed in AD cases may be explained by lower vitamin B12 and higher Hcy levels, respectively. SN - 1661-6758 UR - https://www.unboundmedicine.com/medline/citation/26228832/Buccal_Cytome_Biomarkers_and_Their_Association_with_Plasma_Folate_Vitamin_B12_and_Homocysteine_in_Alzheimer's_Disease_ L2 - https://www.karger.com?DOI=10.1159/000435784 DB - PRIME DP - Unbound Medicine ER -