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Circulating microRNAs as biomarkers of adult Crohn's disease.
Eur J Gastroenterol Hepatol. 2015 Sep; 27(9):1038-44.EJ

Abstract

OBJECTIVE

Previous studies have found a differential expression of microRNAs (miRNAs) in the blood of patients with Crohn's disease (CD) compared with healthy controls. The aim of this study was to identify circulating miRNAs expressed in CD and assess their performance as biomarkers in patients with clinically suspected or known CD.

METHODS

The study consisted of two parts: (a) miRNA profiling: The miRNA expression pattern was examined in six patients with CD and six controls using OpenArray miRNA profiling, and the best miRNAs were selected according to their P-value. (b) Validation cohort: In a well-characterized cohort of 102 patients with suspected or known CD, miRNAs identified by miRNA profiling or selected from previously published studies (hsa-miR-16, hsa-miR-21, hsa-miR-106a, and hsa-miR-140-3p) were measured in plasma using reverse transcription PCR.

RESULTS

miRNA profiling: hsa-miR-369-3p, hsa-miR-376a, hsa-miR-376, hsa-miR-411#, hsa-miR-411, and mmu-miR-379 were downregulated in CD patients compared with the controls; hsa-miR-200c, hsa-miR-181-2#, and hsa-miR-125a-5p were upregulated (P<0.05). Validation cohort: Only hsa-miR-16 was significantly downregulated in patients with CD compared with patients without CD (fold change 0.83, P=0.02). Receiver operating characteristic analyses showed an area under the curve of 0.65. miRNAs could not discriminate inflammatory from stricturing CD or small bowel CD from CD involving the colon.

CONCLUSION

In a clinically relevant cohort of patients, miRNAs in plasma identified in the present and previous studies were inadequate biomarkers for the diagnosis of CD.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Jensen MD
aDepartment of Internal Medicine, Section of Gastroenterology bDepartment of Clinical Biochemistry, Lillebaelt Hospital, Vejle cDepartment of Medical Gastroenterology, Odense University Hospital, Odense, Denmark.
Andersen RF
No affiliation info available
Christensen H
No affiliation info available
Nathan T
No affiliation info available
Kjeldsen J
No affiliation info available
Madsen JS
No affiliation info available

MeSH

AdolescentAdultAgedArea Under CurveCase-Control StudiesCrohn DiseaseFemaleGene Expression ProfilingGenetic MarkersGenetic Predisposition to DiseaseGenetic TestingHumansMaleMicroRNAsMiddle AgedOligonucleotide Array Sequence AnalysisPhenotypePredictive Value of TestsPrognosisROC CurveReal-Time Polymerase Chain ReactionReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionYoung Adult

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

26230660

Clinical Trial Links

Clinical trial which this article describes

Citation

Jensen, Michael D., et al. "Circulating microRNAs as Biomarkers of Adult Crohn's Disease." European Journal of Gastroenterology & Hepatology, vol. 27, no. 9, 2015, pp. 1038-44.
Jensen MD, Andersen RF, Christensen H, et al. Circulating microRNAs as biomarkers of adult Crohn's disease. Eur J Gastroenterol Hepatol. 2015;27(9):1038-44.
Jensen, M. D., Andersen, R. F., Christensen, H., Nathan, T., Kjeldsen, J., & Madsen, J. S. (2015). Circulating microRNAs as biomarkers of adult Crohn's disease. European Journal of Gastroenterology & Hepatology, 27(9), 1038-44. https://doi.org/10.1097/MEG.0000000000000430
Jensen MD, et al. Circulating microRNAs as Biomarkers of Adult Crohn's Disease. Eur J Gastroenterol Hepatol. 2015;27(9):1038-44. PubMed PMID: 26230660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating microRNAs as biomarkers of adult Crohn's disease. AU - Jensen,Michael D, AU - Andersen,Rikke F, AU - Christensen,Henry, AU - Nathan,Torben, AU - Kjeldsen,Jens, AU - Madsen,Jonna S, PY - 2015/8/1/entrez PY - 2015/8/1/pubmed PY - 2016/5/5/medline SP - 1038 EP - 44 JF - European journal of gastroenterology & hepatology JO - Eur J Gastroenterol Hepatol VL - 27 IS - 9 N2 - OBJECTIVE: Previous studies have found a differential expression of microRNAs (miRNAs) in the blood of patients with Crohn's disease (CD) compared with healthy controls. The aim of this study was to identify circulating miRNAs expressed in CD and assess their performance as biomarkers in patients with clinically suspected or known CD. METHODS: The study consisted of two parts: (a) miRNA profiling: The miRNA expression pattern was examined in six patients with CD and six controls using OpenArray miRNA profiling, and the best miRNAs were selected according to their P-value. (b) Validation cohort: In a well-characterized cohort of 102 patients with suspected or known CD, miRNAs identified by miRNA profiling or selected from previously published studies (hsa-miR-16, hsa-miR-21, hsa-miR-106a, and hsa-miR-140-3p) were measured in plasma using reverse transcription PCR. RESULTS: miRNA profiling: hsa-miR-369-3p, hsa-miR-376a, hsa-miR-376, hsa-miR-411#, hsa-miR-411, and mmu-miR-379 were downregulated in CD patients compared with the controls; hsa-miR-200c, hsa-miR-181-2#, and hsa-miR-125a-5p were upregulated (P<0.05). Validation cohort: Only hsa-miR-16 was significantly downregulated in patients with CD compared with patients without CD (fold change 0.83, P=0.02). Receiver operating characteristic analyses showed an area under the curve of 0.65. miRNAs could not discriminate inflammatory from stricturing CD or small bowel CD from CD involving the colon. CONCLUSION: In a clinically relevant cohort of patients, miRNAs in plasma identified in the present and previous studies were inadequate biomarkers for the diagnosis of CD. SN - 1473-5687 UR - https://www.unboundmedicine.com/medline/citation/26230660/Circulating_microRNAs_as_biomarkers_of_adult_Crohn's_disease_ DB - PRIME DP - Unbound Medicine ER -