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Elevated salivary protein in Parkinson's disease and salivary DJ-1 as a potential marker of disease severity.
Parkinsonism Relat Disord. 2015 Oct; 21(10):1251-5.PR

Abstract

INTRODUCTION

There is an urgent need to identify robust biomarkers for Parkinson's disease (PD). Previous studies have shown changes in composition and secretion of saliva in patients with PD, including an increase in salivary DJ-1 concentration. Autonomic dysfunction is a known feature of PD and could contribute to abnormal salivary gland function.

METHODS

In this pilot cross-sectional study, characterisation of the saliva of 16 patients with PD and 22 age-matched controls was performed. Salivary DJ-1 concentration was measured with quantitative immunoblotting; total protein concentration with a BCA assay and spectrophotometry; amylase with an amylase activity assay; albumin with an ELISA and mucin concentration with periodic-acid Schiff staining of SDS-gels.

RESULTS

Patient saliva showed an increase in both total protein concentration (8.4 vs 5.0 mg/ml, p = 0.0002) and DJ-1 concentration (0.84 vs 0.42 μg/ml, p = 0.001), but there was no difference in salivary DJ-1 after adjusting for total protein concentration. In patients, adjusted DJ-1 levels correlated with disease severity measured with the MDS-Unified Parkinson's Disease Rating Scale (p = 0.019). Patient saliva had elevated concentrations of amylase (127 vs 64 units/ml, p = 0.0005) and albumin (110 vs 47μg/ml, p = 0.0003) but not mucins.

CONCLUSIONS

This study suggests that the saliva of patients with PD is different in composition to that of healthy age-matched controls, supporting the notion that saliva may be a good candidate for biomarker discovery in PD. The specific differences suggest that major salivary glands and gingival crevicular fluid may both be sources of additional DJ-1 and protein in patient saliva.

Authors+Show Affiliations

Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom; Mucosal & Salivary Biology Division, Floor 17 Tower Wing, King's College London Dental Institute, London, United Kingdom.Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom; Reta Lila Weston Institute of Neurological Studies, UCL Institute of Neurology, London, United Kingdom.Reta Lila Weston Institute of Neurological Studies, UCL Institute of Neurology, London, United Kingdom.Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom.Mucosal & Salivary Biology Division, Floor 17 Tower Wing, King's College London Dental Institute, London, United Kingdom. Electronic address: gordon.proctor@kcl.ac.uk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26231472

Citation

Masters, Joseph M., et al. "Elevated Salivary Protein in Parkinson's Disease and Salivary DJ-1 as a Potential Marker of Disease Severity." Parkinsonism & Related Disorders, vol. 21, no. 10, 2015, pp. 1251-5.
Masters JM, Noyce AJ, Warner TT, et al. Elevated salivary protein in Parkinson's disease and salivary DJ-1 as a potential marker of disease severity. Parkinsonism Relat Disord. 2015;21(10):1251-5.
Masters, J. M., Noyce, A. J., Warner, T. T., Giovannoni, G., & Proctor, G. B. (2015). Elevated salivary protein in Parkinson's disease and salivary DJ-1 as a potential marker of disease severity. Parkinsonism & Related Disorders, 21(10), 1251-5. https://doi.org/10.1016/j.parkreldis.2015.07.021
Masters JM, et al. Elevated Salivary Protein in Parkinson's Disease and Salivary DJ-1 as a Potential Marker of Disease Severity. Parkinsonism Relat Disord. 2015;21(10):1251-5. PubMed PMID: 26231472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elevated salivary protein in Parkinson's disease and salivary DJ-1 as a potential marker of disease severity. AU - Masters,Joseph M, AU - Noyce,Alastair J, AU - Warner,Thomas T, AU - Giovannoni,Gavin, AU - Proctor,Gordon B, Y1 - 2015/07/23/ PY - 2015/03/31/received PY - 2015/06/11/revised PY - 2015/07/22/accepted PY - 2015/8/2/entrez PY - 2015/8/2/pubmed PY - 2016/8/5/medline KW - Autonomic function KW - Biomarkers KW - DJ-1 KW - Diagnostics KW - Parkinson's disease SP - 1251 EP - 5 JF - Parkinsonism & related disorders JO - Parkinsonism Relat Disord VL - 21 IS - 10 N2 - INTRODUCTION: There is an urgent need to identify robust biomarkers for Parkinson's disease (PD). Previous studies have shown changes in composition and secretion of saliva in patients with PD, including an increase in salivary DJ-1 concentration. Autonomic dysfunction is a known feature of PD and could contribute to abnormal salivary gland function. METHODS: In this pilot cross-sectional study, characterisation of the saliva of 16 patients with PD and 22 age-matched controls was performed. Salivary DJ-1 concentration was measured with quantitative immunoblotting; total protein concentration with a BCA assay and spectrophotometry; amylase with an amylase activity assay; albumin with an ELISA and mucin concentration with periodic-acid Schiff staining of SDS-gels. RESULTS: Patient saliva showed an increase in both total protein concentration (8.4 vs 5.0 mg/ml, p = 0.0002) and DJ-1 concentration (0.84 vs 0.42 μg/ml, p = 0.001), but there was no difference in salivary DJ-1 after adjusting for total protein concentration. In patients, adjusted DJ-1 levels correlated with disease severity measured with the MDS-Unified Parkinson's Disease Rating Scale (p = 0.019). Patient saliva had elevated concentrations of amylase (127 vs 64 units/ml, p = 0.0005) and albumin (110 vs 47μg/ml, p = 0.0003) but not mucins. CONCLUSIONS: This study suggests that the saliva of patients with PD is different in composition to that of healthy age-matched controls, supporting the notion that saliva may be a good candidate for biomarker discovery in PD. The specific differences suggest that major salivary glands and gingival crevicular fluid may both be sources of additional DJ-1 and protein in patient saliva. SN - 1873-5126 UR - https://www.unboundmedicine.com/medline/citation/26231472/Elevated_salivary_protein_in_Parkinson's_disease_and_salivary_DJ_1_as_a_potential_marker_of_disease_severity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1353-8020(15)00312-0 DB - PRIME DP - Unbound Medicine ER -